<i>Mahonia aquifolium</i> Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro
<i>Mahonia aquifolium</i> and its secondary metabolites have been shown to have anticancer potential. We performed MTT, scratch, and colony formation assays; analyzed cell cycle phase distribution and doxorubicin uptake and retention with flow cytometry; and detected alterations in the e...
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MDPI AG
2020-11-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/22/5233 |
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| author | Ana Damjanović Branka Kolundžija Ivana Z. Matić Ana Krivokuća Gordana Zdunić Katarina Šavikin Radmila Janković Jelena Antić Stanković Tatjana P. Stanojković |
| author_facet | Ana Damjanović Branka Kolundžija Ivana Z. Matić Ana Krivokuća Gordana Zdunić Katarina Šavikin Radmila Janković Jelena Antić Stanković Tatjana P. Stanojković |
| author_sort | Ana Damjanović |
| collection | DOAJ |
| description | <i>Mahonia aquifolium</i> and its secondary metabolites have been shown to have anticancer potential. We performed MTT, scratch, and colony formation assays; analyzed cell cycle phase distribution and doxorubicin uptake and retention with flow cytometry; and detected alterations in the expression of genes involved in the formation of cell–cell interactions and migration using quantitative real-time PCR following treatment of lung adenocarcinoma cells with doxorubicin, <i>M. aquifolium</i> extracts, or their combination. MTT assay results suggested strong synergistic effects of the combined treatments, and their application led to an increase in cell numbers in the subG1 phase of the cell cycle. Both extracts were shown to prolong doxorubicin retention time in cancer cells, while the application of doxorubicin/extract combination led to a decrease in <i>MMP9</i> expression. Furthermore, cells treated with doxorubicin/extract combinations were shown to have lower migratory and colony formation potentials than untreated cells or cells treated with doxorubicin alone. The obtained results suggest that nontoxic <i>M. aquifolium</i> extracts can enhance the activity of doxorubicin, thus potentially allowing the application of lower doxorubicin doses in vivo, which may decrease its toxic effects in normal tissues. |
| first_indexed | 2024-03-10T14:57:54Z |
| format | Article |
| id | doaj.art-74e320d701c045038ed2e38440401595 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T14:57:54Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-74e320d701c045038ed2e384404015952023-11-20T20:25:13ZengMDPI AGMolecules1420-30492020-11-012522523310.3390/molecules25225233<i>Mahonia aquifolium</i> Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In VitroAna Damjanović0Branka Kolundžija1Ivana Z. Matić2Ana Krivokuća3Gordana Zdunić4Katarina Šavikin5Radmila Janković6Jelena Antić Stanković7Tatjana P. Stanojković8Department for Experimental Oncology, Institute of Oncology and Radiology of Serbia, 11 000 Belgrade, SerbiaDepartment for Experimental Oncology, Institute of Oncology and Radiology of Serbia, 11 000 Belgrade, SerbiaDepartment for Experimental Oncology, Institute of Oncology and Radiology of Serbia, 11 000 Belgrade, SerbiaDepartment for Experimental Oncology, Institute of Oncology and Radiology of Serbia, 11 000 Belgrade, SerbiaDepartment for Pharmaceutical Investigations and Development, Institute for Medicinal Plant Research, Dr. Josif Pančić, 11 070 Belgrade, SerbiaDepartment for Pharmaceutical Investigations and Development, Institute for Medicinal Plant Research, Dr. Josif Pančić, 11 070 Belgrade, SerbiaDepartment for Experimental Oncology, Institute of Oncology and Radiology of Serbia, 11 000 Belgrade, SerbiaDepartment for Microbiology and Immunology, Faculty of Pharmacy, University of Belgrade, 11 221 Belgrade, SerbiaDepartment for Experimental Oncology, Institute of Oncology and Radiology of Serbia, 11 000 Belgrade, Serbia<i>Mahonia aquifolium</i> and its secondary metabolites have been shown to have anticancer potential. We performed MTT, scratch, and colony formation assays; analyzed cell cycle phase distribution and doxorubicin uptake and retention with flow cytometry; and detected alterations in the expression of genes involved in the formation of cell–cell interactions and migration using quantitative real-time PCR following treatment of lung adenocarcinoma cells with doxorubicin, <i>M. aquifolium</i> extracts, or their combination. MTT assay results suggested strong synergistic effects of the combined treatments, and their application led to an increase in cell numbers in the subG1 phase of the cell cycle. Both extracts were shown to prolong doxorubicin retention time in cancer cells, while the application of doxorubicin/extract combination led to a decrease in <i>MMP9</i> expression. Furthermore, cells treated with doxorubicin/extract combinations were shown to have lower migratory and colony formation potentials than untreated cells or cells treated with doxorubicin alone. The obtained results suggest that nontoxic <i>M. aquifolium</i> extracts can enhance the activity of doxorubicin, thus potentially allowing the application of lower doxorubicin doses in vivo, which may decrease its toxic effects in normal tissues.https://www.mdpi.com/1420-3049/25/22/5233doxorubicin<i>Mahonia aquifolium</i>matrix metalloproteinasescytotoxicityhuman lung adenocarcinoma |
| spellingShingle | Ana Damjanović Branka Kolundžija Ivana Z. Matić Ana Krivokuća Gordana Zdunić Katarina Šavikin Radmila Janković Jelena Antić Stanković Tatjana P. Stanojković <i>Mahonia aquifolium</i> Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro Molecules doxorubicin <i>Mahonia aquifolium</i> matrix metalloproteinases cytotoxicity human lung adenocarcinoma |
| title | <i>Mahonia aquifolium</i> Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro |
| title_full | <i>Mahonia aquifolium</i> Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro |
| title_fullStr | <i>Mahonia aquifolium</i> Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro |
| title_full_unstemmed | <i>Mahonia aquifolium</i> Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro |
| title_short | <i>Mahonia aquifolium</i> Extracts Promote Doxorubicin Effects against Lung Adenocarcinoma Cells In Vitro |
| title_sort | i mahonia aquifolium i extracts promote doxorubicin effects against lung adenocarcinoma cells in vitro |
| topic | doxorubicin <i>Mahonia aquifolium</i> matrix metalloproteinases cytotoxicity human lung adenocarcinoma |
| url | https://www.mdpi.com/1420-3049/25/22/5233 |
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