A Ubiquitously Expressed UDP-Glucosyltransferase, <i>UGT74J1</i>, Controls Basal Salicylic Acid Levels in Rice
Salicylic acid (SA) is a phytohormone that regulates a variety of physiological and developmental processes, including disease resistance. SA is a key signaling component in the immune response of many plant species. However, the mechanism underlying SA-mediated immunity is obscure in rice (<i>...
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MDPI AG
2021-09-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/10/9/1875 |
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| author | Daisuke Tezuka Hideyuki Matsuura Wataru Saburi Haruhide Mori Ryozo Imai |
| author_facet | Daisuke Tezuka Hideyuki Matsuura Wataru Saburi Haruhide Mori Ryozo Imai |
| author_sort | Daisuke Tezuka |
| collection | DOAJ |
| description | Salicylic acid (SA) is a phytohormone that regulates a variety of physiological and developmental processes, including disease resistance. SA is a key signaling component in the immune response of many plant species. However, the mechanism underlying SA-mediated immunity is obscure in rice (<i>Oryza sativa</i>). Prior analysis revealed a correlation between basal SA level and blast resistance in a range of rice varieties. This suggested that resistance might be improved by increasing basal SA level. Here, we identified a novel UDP-glucosyltransferase gene, <i>UGT74J1</i>, which is expressed ubiquitously throughout plant development. Mutants of <i>UGT74J1</i> generated by genome editing accumulated high levels of SA under non-stressed conditions, indicating that <i>UGT74J1</i> is a key enzyme for SA homeostasis in rice. Microarray analysis revealed that the <i>ugt74j1</i> mutants constitutively overexpressed a set of pathogenesis-related (PR) genes. An inoculation assay demonstrated that these mutants had increased resistance against rice blast, but they also exhibited stunted growth phenotypes. To our knowledge, this is the first report of a rice mutant displaying SA overaccumulation. |
| first_indexed | 2024-03-10T07:17:24Z |
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| id | doaj.art-74e3bfc89b334a34ac4f6b07d350ec82 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T07:17:24Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-74e3bfc89b334a34ac4f6b07d350ec822023-11-22T14:53:05ZengMDPI AGPlants2223-77472021-09-01109187510.3390/plants10091875A Ubiquitously Expressed UDP-Glucosyltransferase, <i>UGT74J1</i>, Controls Basal Salicylic Acid Levels in RiceDaisuke Tezuka0Hideyuki Matsuura1Wataru Saburi2Haruhide Mori3Ryozo Imai4Institute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Kannondai, Tsukuba 305-8604, JapanResearch Faculty of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, JapanResearch Faculty of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, JapanResearch Faculty of Agriculture, Hokkaido University, Kita-ku, Sapporo 060-8589, JapanInstitute of Agrobiological Sciences, National Agriculture and Food Research Organization (NARO), Kannondai, Tsukuba 305-8604, JapanSalicylic acid (SA) is a phytohormone that regulates a variety of physiological and developmental processes, including disease resistance. SA is a key signaling component in the immune response of many plant species. However, the mechanism underlying SA-mediated immunity is obscure in rice (<i>Oryza sativa</i>). Prior analysis revealed a correlation between basal SA level and blast resistance in a range of rice varieties. This suggested that resistance might be improved by increasing basal SA level. Here, we identified a novel UDP-glucosyltransferase gene, <i>UGT74J1</i>, which is expressed ubiquitously throughout plant development. Mutants of <i>UGT74J1</i> generated by genome editing accumulated high levels of SA under non-stressed conditions, indicating that <i>UGT74J1</i> is a key enzyme for SA homeostasis in rice. Microarray analysis revealed that the <i>ugt74j1</i> mutants constitutively overexpressed a set of pathogenesis-related (PR) genes. An inoculation assay demonstrated that these mutants had increased resistance against rice blast, but they also exhibited stunted growth phenotypes. To our knowledge, this is the first report of a rice mutant displaying SA overaccumulation.https://www.mdpi.com/2223-7747/10/9/1875salicylic acidglycosylationUDP-glucosyltransferasedisease resistance<i>Pyricularia oryzae</i> |
| spellingShingle | Daisuke Tezuka Hideyuki Matsuura Wataru Saburi Haruhide Mori Ryozo Imai A Ubiquitously Expressed UDP-Glucosyltransferase, <i>UGT74J1</i>, Controls Basal Salicylic Acid Levels in Rice Plants salicylic acid glycosylation UDP-glucosyltransferase disease resistance <i>Pyricularia oryzae</i> |
| title | A Ubiquitously Expressed UDP-Glucosyltransferase, <i>UGT74J1</i>, Controls Basal Salicylic Acid Levels in Rice |
| title_full | A Ubiquitously Expressed UDP-Glucosyltransferase, <i>UGT74J1</i>, Controls Basal Salicylic Acid Levels in Rice |
| title_fullStr | A Ubiquitously Expressed UDP-Glucosyltransferase, <i>UGT74J1</i>, Controls Basal Salicylic Acid Levels in Rice |
| title_full_unstemmed | A Ubiquitously Expressed UDP-Glucosyltransferase, <i>UGT74J1</i>, Controls Basal Salicylic Acid Levels in Rice |
| title_short | A Ubiquitously Expressed UDP-Glucosyltransferase, <i>UGT74J1</i>, Controls Basal Salicylic Acid Levels in Rice |
| title_sort | ubiquitously expressed udp glucosyltransferase i ugt74j1 i controls basal salicylic acid levels in rice |
| topic | salicylic acid glycosylation UDP-glucosyltransferase disease resistance <i>Pyricularia oryzae</i> |
| url | https://www.mdpi.com/2223-7747/10/9/1875 |
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