| Summary: | Time domain nuclear magnetic resonance (TD–NMR) has been widely applied in plant science in the last four decades. Several TD–NMR instruments and methods have been developed for laboratory, green-house, and field studies. This mini-review focuses on the recent TD–NMR pulse sequences applied in plant science. One of the sequences measures the transverse relaxation time (T<sub>2</sub>) with minimal sample heating, using a lower refocusing flip angle and consequently lower specific absorption rate than that of conventional CPMG. Other sequences are based on a continuous wave free precession (CWFP) regime used to enhance the signal-to-noise ratio, to measure longitudinal (T<sub>1</sub>) and transverse relaxation time in a single shot experiment, and as alternative 2D pulse sequences to obtain T<sub>1</sub>–T<sub>2</sub> and diffusion-T<sub>1</sub> correlation maps. This review also presents some applications of these sequences in plant science.
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