OsbZIP01 Affects Plant Growth and Development by Regulating OsSD1 in Rice
Abstracts: As the ‘Green Revolution’ gene, SD1 (encoding GA20ox2), has been widely applied to improve yield in rice breeding. However, research on its transcriptional regulation is limited. Here, we identified a transcription factor OsbZIP01, which can suppress the expression of SD1 and regulate gib...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Rice Science |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1672630823001178 |
| _version_ | 1827356729807994880 |
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| author | Dong Xinli Zhou Yang Zhang Yaqi Rong Fuxi Du Jiahong Hong Zheyuan H.U. Peisong Lü Yusong |
| author_facet | Dong Xinli Zhou Yang Zhang Yaqi Rong Fuxi Du Jiahong Hong Zheyuan H.U. Peisong Lü Yusong |
| author_sort | Dong Xinli |
| collection | DOAJ |
| description | Abstracts: As the ‘Green Revolution’ gene, SD1 (encoding GA20ox2), has been widely applied to improve yield in rice breeding. However, research on its transcriptional regulation is limited. Here, we identified a transcription factor OsbZIP01, which can suppress the expression of SD1 and regulate gibberellin (GA) biosynthesis in rice. Knockout mutants of OsbZIP01 exhibited increased plant height, while the over- expression lines showed a semi-dwarf phenotype and diminished germination rate. Furthermore, the semi-dwarf phenotype of OE-bZIP01, was caused by the reduced internode length, which was accompanied by a thin stem width. The predominant expression of OsbZIP01 was observed in leaves and sheaths. OsbZIP01 protein was localized in the nucleus and showed transcriptional repression activity. In addition, OsbZIP01 could directly bind to the promoter of the OsSD1 gene, and inhibit its transcription. The semi-dwarf phenotype of OE-bZIP01 could be rescued by exogenous GA3. Meanwhile, the bzip01 sd1 double mutant showed a shorter shoot length compared with the wild type, indicating that OsbZIP01 regulated plant growth mainly through the GA biosynthesis pathway. Collectively, OsbZIP01 negatively regulates GA biosynthesis by restraining SD1 transcription, thereby affecting plant growth and development. |
| first_indexed | 2024-03-08T05:14:11Z |
| format | Article |
| id | doaj.art-74ea772228b84bf994340f1284fd2326 |
| institution | Directory Open Access Journal |
| issn | 1672-6308 |
| language | English |
| last_indexed | 2024-03-08T05:14:11Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Rice Science |
| spelling | doaj.art-74ea772228b84bf994340f1284fd23262024-02-07T04:44:14ZengElsevierRice Science1672-63082024-01-013117786OsbZIP01 Affects Plant Growth and Development by Regulating OsSD1 in RiceDong Xinli0Zhou Yang1Zhang Yaqi2Rong Fuxi3Du Jiahong4Hong Zheyuan5H.U. Peisong6Lü Yusong7College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China;; State Key Laboratory of Rice Biology and Breeding / China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China;College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China;College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China;College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China;College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China;College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China;College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China;; State Key Laboratory of Rice Biology and Breeding / China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China;College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, China;; State Key Laboratory of Rice Biology and Breeding / China National Center for Rice Improvement, China National Rice Research Institute, Hangzhou 310006, China;; Corresponding author.Abstracts: As the ‘Green Revolution’ gene, SD1 (encoding GA20ox2), has been widely applied to improve yield in rice breeding. However, research on its transcriptional regulation is limited. Here, we identified a transcription factor OsbZIP01, which can suppress the expression of SD1 and regulate gibberellin (GA) biosynthesis in rice. Knockout mutants of OsbZIP01 exhibited increased plant height, while the over- expression lines showed a semi-dwarf phenotype and diminished germination rate. Furthermore, the semi-dwarf phenotype of OE-bZIP01, was caused by the reduced internode length, which was accompanied by a thin stem width. The predominant expression of OsbZIP01 was observed in leaves and sheaths. OsbZIP01 protein was localized in the nucleus and showed transcriptional repression activity. In addition, OsbZIP01 could directly bind to the promoter of the OsSD1 gene, and inhibit its transcription. The semi-dwarf phenotype of OE-bZIP01 could be rescued by exogenous GA3. Meanwhile, the bzip01 sd1 double mutant showed a shorter shoot length compared with the wild type, indicating that OsbZIP01 regulated plant growth mainly through the GA biosynthesis pathway. Collectively, OsbZIP01 negatively regulates GA biosynthesis by restraining SD1 transcription, thereby affecting plant growth and development.http://www.sciencedirect.com/science/article/pii/S1672630823001178OsbZIP01SD1gibberellin biosynthesisdwarf and germination |
| spellingShingle | Dong Xinli Zhou Yang Zhang Yaqi Rong Fuxi Du Jiahong Hong Zheyuan H.U. Peisong Lü Yusong OsbZIP01 Affects Plant Growth and Development by Regulating OsSD1 in Rice Rice Science OsbZIP01 SD1 gibberellin biosynthesis dwarf and germination |
| title | OsbZIP01 Affects Plant Growth and Development by Regulating OsSD1 in Rice |
| title_full | OsbZIP01 Affects Plant Growth and Development by Regulating OsSD1 in Rice |
| title_fullStr | OsbZIP01 Affects Plant Growth and Development by Regulating OsSD1 in Rice |
| title_full_unstemmed | OsbZIP01 Affects Plant Growth and Development by Regulating OsSD1 in Rice |
| title_short | OsbZIP01 Affects Plant Growth and Development by Regulating OsSD1 in Rice |
| title_sort | osbzip01 affects plant growth and development by regulating ossd1 in rice |
| topic | OsbZIP01 SD1 gibberellin biosynthesis dwarf and germination |
| url | http://www.sciencedirect.com/science/article/pii/S1672630823001178 |
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