Effect of Epidermal Growth Factor (EGF) on Cryopreserved Piedmontese Bull Semen Characteristics
The purpose of this study was to determine the effect on fresh and post-thaw beef bull semen quality of the supplementation of epidermal growth factor (EGF) to the semen extender at various concentrations (0-control, 50, 100, 200, and 400 ng/mL). For 8 weeks, sperm was collected from four fertile bu...
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MDPI AG
2022-11-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/12/22/3179 |
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| author | Ahmed R. Alkhawagah Alessandro Ricci Penelope Banchi Nicola A. Martino Mariagrazia Lucia Poletto Gian Guido Donato Tiziana Nervo Leila Vincenti |
| author_facet | Ahmed R. Alkhawagah Alessandro Ricci Penelope Banchi Nicola A. Martino Mariagrazia Lucia Poletto Gian Guido Donato Tiziana Nervo Leila Vincenti |
| author_sort | Ahmed R. Alkhawagah |
| collection | DOAJ |
| description | The purpose of this study was to determine the effect on fresh and post-thaw beef bull semen quality of the supplementation of epidermal growth factor (EGF) to the semen extender at various concentrations (0-control, 50, 100, 200, and 400 ng/mL). For 8 weeks, sperm was collected from four fertile bulls, yielding a total of 32 ejaculates. Semen samples were pooled, diluted with Bullxcell<sup>®</sup> extender, and then cooled, equilibrated, and frozen. After thawing, semen was tested for motility and velocity parameters. Furthermore, semen was evaluated for vitality, integrity, mitochondrial and antioxidant (SOD) activities, mucus penetration distance, and in vitro fertilizing capability. The supplementation with EGF prior to cryopreservation improved the total sperm motility at various concentrations over long incubation periods (from 1 to 4 h). Interestingly, EGF addition improved both progressive and rapid motility, particularly at 50, 200, and 400 ng/mL. In addition, EGF, primarily at 200 and 400 ng/mL, significantly increased several velocity parameters after different incubation periods. We can conclude that adding EGF to bull sperm extender before cryopreservation has a positive stimulatory effect on sperm motility without affecting vitality, integrity, or in vitro fertilizing capability. |
| first_indexed | 2024-03-09T18:31:17Z |
| format | Article |
| id | doaj.art-74ed51f1d1974bd0bd435792b6b8188d |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T18:31:17Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-74ed51f1d1974bd0bd435792b6b8188d2023-11-24T07:29:31ZengMDPI AGAnimals2076-26152022-11-011222317910.3390/ani12223179Effect of Epidermal Growth Factor (EGF) on Cryopreserved Piedmontese Bull Semen CharacteristicsAhmed R. Alkhawagah0Alessandro Ricci1Penelope Banchi2Nicola A. Martino3Mariagrazia Lucia Poletto4Gian Guido Donato5Tiziana Nervo6Leila Vincenti7Theriogenology Department, Faculty of Veterinary Medicine, Benha University, Banha 11629, EgyptDepartment of Veterinary Science, University of Torino, Largo Braccini 2, 10095 Grugliasco, ItalyDepartment of Veterinary Science, University of Torino, Largo Braccini 2, 10095 Grugliasco, ItalyDepartment of Biosciences, Biotechnologies & Biopharmaceutics, University of Bari Aldo Moro, Via Edoardo Orabona, 70125 Bari, ItalyDepartment of Veterinary Science, University of Torino, Largo Braccini 2, 10095 Grugliasco, ItalyDepartment of Veterinary Science, University of Torino, Largo Braccini 2, 10095 Grugliasco, ItalyDepartment of Veterinary Science, University of Torino, Largo Braccini 2, 10095 Grugliasco, ItalyDepartment of Veterinary Science, University of Torino, Largo Braccini 2, 10095 Grugliasco, ItalyThe purpose of this study was to determine the effect on fresh and post-thaw beef bull semen quality of the supplementation of epidermal growth factor (EGF) to the semen extender at various concentrations (0-control, 50, 100, 200, and 400 ng/mL). For 8 weeks, sperm was collected from four fertile bulls, yielding a total of 32 ejaculates. Semen samples were pooled, diluted with Bullxcell<sup>®</sup> extender, and then cooled, equilibrated, and frozen. After thawing, semen was tested for motility and velocity parameters. Furthermore, semen was evaluated for vitality, integrity, mitochondrial and antioxidant (SOD) activities, mucus penetration distance, and in vitro fertilizing capability. The supplementation with EGF prior to cryopreservation improved the total sperm motility at various concentrations over long incubation periods (from 1 to 4 h). Interestingly, EGF addition improved both progressive and rapid motility, particularly at 50, 200, and 400 ng/mL. In addition, EGF, primarily at 200 and 400 ng/mL, significantly increased several velocity parameters after different incubation periods. We can conclude that adding EGF to bull sperm extender before cryopreservation has a positive stimulatory effect on sperm motility without affecting vitality, integrity, or in vitro fertilizing capability.https://www.mdpi.com/2076-2615/12/22/3179bull spermatozoacryopreservationepidermal growth factorsperm qualityDNAmitochondrial activity |
| spellingShingle | Ahmed R. Alkhawagah Alessandro Ricci Penelope Banchi Nicola A. Martino Mariagrazia Lucia Poletto Gian Guido Donato Tiziana Nervo Leila Vincenti Effect of Epidermal Growth Factor (EGF) on Cryopreserved Piedmontese Bull Semen Characteristics Animals bull spermatozoa cryopreservation epidermal growth factor sperm quality DNA mitochondrial activity |
| title | Effect of Epidermal Growth Factor (EGF) on Cryopreserved Piedmontese Bull Semen Characteristics |
| title_full | Effect of Epidermal Growth Factor (EGF) on Cryopreserved Piedmontese Bull Semen Characteristics |
| title_fullStr | Effect of Epidermal Growth Factor (EGF) on Cryopreserved Piedmontese Bull Semen Characteristics |
| title_full_unstemmed | Effect of Epidermal Growth Factor (EGF) on Cryopreserved Piedmontese Bull Semen Characteristics |
| title_short | Effect of Epidermal Growth Factor (EGF) on Cryopreserved Piedmontese Bull Semen Characteristics |
| title_sort | effect of epidermal growth factor egf on cryopreserved piedmontese bull semen characteristics |
| topic | bull spermatozoa cryopreservation epidermal growth factor sperm quality DNA mitochondrial activity |
| url | https://www.mdpi.com/2076-2615/12/22/3179 |
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