Polymer-Induced Swelling of Solid-Supported Lipid Membranes
In this paper, we study the interaction of charged polymers with solid-supported 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes by in-situ neutron reflectivity. We observe an enormous swelling of the oligolamellar lipid bilayer stacks after incubation in solutions of poly(allylamine hy...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-12-01
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| Series: | Membranes |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2077-0375/6/1/2 |
| _version_ | 1797717537641725952 |
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| author | Martin Kreuzer Marcus Trapp Reiner Dahint Roland Steitz |
| author_facet | Martin Kreuzer Marcus Trapp Reiner Dahint Roland Steitz |
| author_sort | Martin Kreuzer |
| collection | DOAJ |
| description | In this paper, we study the interaction of charged polymers with solid-supported 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes by in-situ neutron reflectivity. We observe an enormous swelling of the oligolamellar lipid bilayer stacks after incubation in solutions of poly(allylamine hydrochloride) (PAH) in D2O. The positively charged polyelectrolyte molecules interact with the lipid bilayers and induce a drastic increase in their d-spacing by a factor of ~4. Temperature, time, and pH influence the swollen interfacial lipid linings. From our study, we conclude that electrostatic interactions introduced by the adsorbed PAH are the main cause for the drastic swelling of the lipid coatings. The DMPC membrane stacks do not detach from their solid support at T > Tm. Steric interactions, also introduced by the PAH molecules, are held responsible for the stabilizing effect. We believe that this novel system offers great potential for fundamental studies of biomembrane properties, keeping the membrane’s natural fluidity and freedom, decoupled from a solid support at physiological conditions. |
| first_indexed | 2024-03-12T08:38:48Z |
| format | Article |
| id | doaj.art-74efc540a0c14874b44cdb1b8bfd96b6 |
| institution | Directory Open Access Journal |
| issn | 2077-0375 |
| language | English |
| last_indexed | 2024-03-12T08:38:48Z |
| publishDate | 2015-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Membranes |
| spelling | doaj.art-74efc540a0c14874b44cdb1b8bfd96b62023-09-02T17:04:09ZengMDPI AGMembranes2077-03752015-12-0161210.3390/membranes6010002membranes6010002Polymer-Induced Swelling of Solid-Supported Lipid MembranesMartin Kreuzer0Marcus Trapp1Reiner Dahint2Roland Steitz3Helmholtz-Zentrum Berlin für Materialien und Energie GmbH, Hahn-Meitner-Platz 1, Berlin 14109, GermanyHelmholtz-Zentrum Berlin für Materialien und Energie GmbH, Hahn-Meitner-Platz 1, Berlin 14109, GermanyAngewandte Physikalische Chemie, Ruprecht-Karls-Universität Heidelberg, Im Neuenheimer Feld 253, Heidelberg 69120, GermanyHelmholtz-Zentrum Berlin für Materialien und Energie GmbH, Hahn-Meitner-Platz 1, Berlin 14109, GermanyIn this paper, we study the interaction of charged polymers with solid-supported 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) membranes by in-situ neutron reflectivity. We observe an enormous swelling of the oligolamellar lipid bilayer stacks after incubation in solutions of poly(allylamine hydrochloride) (PAH) in D2O. The positively charged polyelectrolyte molecules interact with the lipid bilayers and induce a drastic increase in their d-spacing by a factor of ~4. Temperature, time, and pH influence the swollen interfacial lipid linings. From our study, we conclude that electrostatic interactions introduced by the adsorbed PAH are the main cause for the drastic swelling of the lipid coatings. The DMPC membrane stacks do not detach from their solid support at T > Tm. Steric interactions, also introduced by the PAH molecules, are held responsible for the stabilizing effect. We believe that this novel system offers great potential for fundamental studies of biomembrane properties, keeping the membrane’s natural fluidity and freedom, decoupled from a solid support at physiological conditions.http://www.mdpi.com/2077-0375/6/1/2lipidmembranespolymerneutron reflectivity |
| spellingShingle | Martin Kreuzer Marcus Trapp Reiner Dahint Roland Steitz Polymer-Induced Swelling of Solid-Supported Lipid Membranes Membranes lipid membranes polymer neutron reflectivity |
| title | Polymer-Induced Swelling of Solid-Supported Lipid Membranes |
| title_full | Polymer-Induced Swelling of Solid-Supported Lipid Membranes |
| title_fullStr | Polymer-Induced Swelling of Solid-Supported Lipid Membranes |
| title_full_unstemmed | Polymer-Induced Swelling of Solid-Supported Lipid Membranes |
| title_short | Polymer-Induced Swelling of Solid-Supported Lipid Membranes |
| title_sort | polymer induced swelling of solid supported lipid membranes |
| topic | lipid membranes polymer neutron reflectivity |
| url | http://www.mdpi.com/2077-0375/6/1/2 |
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