Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae.
Microsporidium Nosema ceranae is well known for exerting a negative impact on honey bee health, including down-regulation of immunoregulatory genes. Protein nutrition has been proven to have beneficial effects on bee immunity and other aspects of bee health. Bearing this in mind, the aim of our stud...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5678887?pdf=render |
| _version_ | 1819083378092146688 |
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| author | Uros Glavinic Biljana Stankovic Vladimir Draskovic Jevrosima Stevanovic Tamas Petrovic Nada Lakic Zoran Stanimirovic |
| author_facet | Uros Glavinic Biljana Stankovic Vladimir Draskovic Jevrosima Stevanovic Tamas Petrovic Nada Lakic Zoran Stanimirovic |
| author_sort | Uros Glavinic |
| collection | DOAJ |
| description | Microsporidium Nosema ceranae is well known for exerting a negative impact on honey bee health, including down-regulation of immunoregulatory genes. Protein nutrition has been proven to have beneficial effects on bee immunity and other aspects of bee health. Bearing this in mind, the aim of our study was to evaluate the potential of a dietary amino acid and vitamin complex "BEEWELL AminoPlus" to protect honey bees from immunosuppression induced by N. ceranae. In a laboratory experiment bees were infected with N. ceranae and treated with supplement on first, third, sixth and ninth day after emergence. The expression of genes for immune-related peptides (abaecin, apidaecin, hymenoptaecin, defensin and vitellogenin) was compared between groups. The results revealed significantly lower (p<0.01 or p<0.001) numbers of Nosema spores in supplemented groups than in the control especially on day 12 post infection. With the exception of abacein, the expression levels of immune-related peptides were significantly suppressed (p<0.01 or p<0.001) in control group on the 12th day post infection, compared to bees that received the supplement. It was supposed that N. ceranae had a negative impact on bee immunity and that the tested amino acid and vitamin complex modified the expression of immune-related genes in honey bees compromised by infection, suggesting immune-stimulation that reflects in the increase in resistance to diseases and reduced bee mortality. The supplement exerted best efficacy when applied simultaneously with Nosema infection, which can help us to assume the most suitable period for its application in the hive. |
| first_indexed | 2024-12-21T20:31:37Z |
| format | Article |
| id | doaj.art-74f1333a6b4549e1bc3bdda533b9cb76 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T20:31:37Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-74f1333a6b4549e1bc3bdda533b9cb762022-12-21T18:51:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011211e018772610.1371/journal.pone.0187726Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae.Uros GlavinicBiljana StankovicVladimir DraskovicJevrosima StevanovicTamas PetrovicNada LakicZoran StanimirovicMicrosporidium Nosema ceranae is well known for exerting a negative impact on honey bee health, including down-regulation of immunoregulatory genes. Protein nutrition has been proven to have beneficial effects on bee immunity and other aspects of bee health. Bearing this in mind, the aim of our study was to evaluate the potential of a dietary amino acid and vitamin complex "BEEWELL AminoPlus" to protect honey bees from immunosuppression induced by N. ceranae. In a laboratory experiment bees were infected with N. ceranae and treated with supplement on first, third, sixth and ninth day after emergence. The expression of genes for immune-related peptides (abaecin, apidaecin, hymenoptaecin, defensin and vitellogenin) was compared between groups. The results revealed significantly lower (p<0.01 or p<0.001) numbers of Nosema spores in supplemented groups than in the control especially on day 12 post infection. With the exception of abacein, the expression levels of immune-related peptides were significantly suppressed (p<0.01 or p<0.001) in control group on the 12th day post infection, compared to bees that received the supplement. It was supposed that N. ceranae had a negative impact on bee immunity and that the tested amino acid and vitamin complex modified the expression of immune-related genes in honey bees compromised by infection, suggesting immune-stimulation that reflects in the increase in resistance to diseases and reduced bee mortality. The supplement exerted best efficacy when applied simultaneously with Nosema infection, which can help us to assume the most suitable period for its application in the hive.http://europepmc.org/articles/PMC5678887?pdf=render |
| spellingShingle | Uros Glavinic Biljana Stankovic Vladimir Draskovic Jevrosima Stevanovic Tamas Petrovic Nada Lakic Zoran Stanimirovic Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae. PLoS ONE |
| title | Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae. |
| title_full | Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae. |
| title_fullStr | Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae. |
| title_full_unstemmed | Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae. |
| title_short | Dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by Nosema ceranae. |
| title_sort | dietary amino acid and vitamin complex protects honey bee from immunosuppression caused by nosema ceranae |
| url | http://europepmc.org/articles/PMC5678887?pdf=render |
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