ELISA and Chemiluminescent Enzyme Immunoassay for Sensitive and Specific Determination of Lead (II) in Water, Food and Feed Samples
Lead is a heavy metal with increasing public health concerns on its accumulation in the food chain and environment. Immunoassays for the quantitative measurement of environmental heavy metals offer numerous advantages over other traditional methods. ELISA and chemiluminescent enzyme immunoassay (CLE...
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MDPI AG
2020-03-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/9/3/305 |
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| author | Long Xu Xiao-yi Suo Qi Zhang Xin-ping Li Chen Chen Xiao-ying Zhang |
| author_facet | Long Xu Xiao-yi Suo Qi Zhang Xin-ping Li Chen Chen Xiao-ying Zhang |
| author_sort | Long Xu |
| collection | DOAJ |
| description | Lead is a heavy metal with increasing public health concerns on its accumulation in the food chain and environment. Immunoassays for the quantitative measurement of environmental heavy metals offer numerous advantages over other traditional methods. ELISA and chemiluminescent enzyme immunoassay (CLEIA), based on the mAb we generated, were developed for the detection of lead (II). In total, 50% inhibitory concentrations (IC<sub>50</sub>) of lead (II) were 9.4 ng/mL (ELISA) and 1.4 ng/mL (CLEIA); the limits of detection (LOD) were 0.7 ng/mL (ic-ELISA) and 0.1 ng/mL (ic-CLEIA), respectively. Cross-reactivities of the mAb toward other metal ions were less than 0.943%, indicating that the obtained mAb has high sensitivity and specificity. The recovery rates were 82.1%−108.3% (ic-ELISA) and 80.1%−98.8% (ic-CLEIA), respectively. The developed methods are feasible for the determination of trace lead (II) in various samples with high sensitivity, specificity, fastness, simplicity and accuracy. |
| first_indexed | 2024-12-19T04:03:37Z |
| format | Article |
| id | doaj.art-74f238336d3340b89ef6794eb2daf0f3 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-12-19T04:03:37Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-74f238336d3340b89ef6794eb2daf0f32022-12-21T20:36:36ZengMDPI AGFoods2304-81582020-03-019330510.3390/foods9030305foods9030305ELISA and Chemiluminescent Enzyme Immunoassay for Sensitive and Specific Determination of Lead (II) in Water, Food and Feed SamplesLong Xu0Xiao-yi Suo1Qi Zhang2Xin-ping Li3Chen Chen4Xiao-ying Zhang5College of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaCollege of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaCollege of Veterinary Medicine, Northwest A&F University, Yangling 712100, ChinaCollege of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaCollege of Biological Science and Engineering, Shaanxi University of Technology, Hanzhong 723000, ChinaLead is a heavy metal with increasing public health concerns on its accumulation in the food chain and environment. Immunoassays for the quantitative measurement of environmental heavy metals offer numerous advantages over other traditional methods. ELISA and chemiluminescent enzyme immunoassay (CLEIA), based on the mAb we generated, were developed for the detection of lead (II). In total, 50% inhibitory concentrations (IC<sub>50</sub>) of lead (II) were 9.4 ng/mL (ELISA) and 1.4 ng/mL (CLEIA); the limits of detection (LOD) were 0.7 ng/mL (ic-ELISA) and 0.1 ng/mL (ic-CLEIA), respectively. Cross-reactivities of the mAb toward other metal ions were less than 0.943%, indicating that the obtained mAb has high sensitivity and specificity. The recovery rates were 82.1%−108.3% (ic-ELISA) and 80.1%−98.8% (ic-CLEIA), respectively. The developed methods are feasible for the determination of trace lead (II) in various samples with high sensitivity, specificity, fastness, simplicity and accuracy.https://www.mdpi.com/2304-8158/9/3/305lead (ii)elisamonoclonal antibody (mab)isothiocyanobenzyl-edta (itcbe)chemiluminescent enzyme immunoassay (cleia) |
| spellingShingle | Long Xu Xiao-yi Suo Qi Zhang Xin-ping Li Chen Chen Xiao-ying Zhang ELISA and Chemiluminescent Enzyme Immunoassay for Sensitive and Specific Determination of Lead (II) in Water, Food and Feed Samples Foods lead (ii) elisa monoclonal antibody (mab) isothiocyanobenzyl-edta (itcbe) chemiluminescent enzyme immunoassay (cleia) |
| title | ELISA and Chemiluminescent Enzyme Immunoassay for Sensitive and Specific Determination of Lead (II) in Water, Food and Feed Samples |
| title_full | ELISA and Chemiluminescent Enzyme Immunoassay for Sensitive and Specific Determination of Lead (II) in Water, Food and Feed Samples |
| title_fullStr | ELISA and Chemiluminescent Enzyme Immunoassay for Sensitive and Specific Determination of Lead (II) in Water, Food and Feed Samples |
| title_full_unstemmed | ELISA and Chemiluminescent Enzyme Immunoassay for Sensitive and Specific Determination of Lead (II) in Water, Food and Feed Samples |
| title_short | ELISA and Chemiluminescent Enzyme Immunoassay for Sensitive and Specific Determination of Lead (II) in Water, Food and Feed Samples |
| title_sort | elisa and chemiluminescent enzyme immunoassay for sensitive and specific determination of lead ii in water food and feed samples |
| topic | lead (ii) elisa monoclonal antibody (mab) isothiocyanobenzyl-edta (itcbe) chemiluminescent enzyme immunoassay (cleia) |
| url | https://www.mdpi.com/2304-8158/9/3/305 |
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