Role of Phage Capsid in the Resistance to UV-C Radiations
The conformational variation of the viral capsid structure plays an essential role both for the environmental resistance and acid nuclear release during cellular infection. The aim of this study was to evaluate how capsid rearrangement in engineered phages of M13 protects viral DNA and peptide bonds...
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MDPI AG
2021-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/7/3408 |
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| author | Laura Maria De Plano Domenico Franco Maria Giovanna Rizzo Vincenzo Zammuto Concetta Gugliandolo Letteria Silipigni Lorenzo Torrisi Salvatore P. P. Guglielmino |
| author_facet | Laura Maria De Plano Domenico Franco Maria Giovanna Rizzo Vincenzo Zammuto Concetta Gugliandolo Letteria Silipigni Lorenzo Torrisi Salvatore P. P. Guglielmino |
| author_sort | Laura Maria De Plano |
| collection | DOAJ |
| description | The conformational variation of the viral capsid structure plays an essential role both for the environmental resistance and acid nuclear release during cellular infection. The aim of this study was to evaluate how capsid rearrangement in engineered phages of M13 protects viral DNA and peptide bonds from damage induced by UV-C radiation. From in silico 3D modelling analysis, two M13 engineered phage clones, namely P9b and 12III1, were chosen for (i) chemical features of amino acids sequences, (ii) rearrangements in the secondary structure of their pVIII proteins and (iii) in turn the interactions involved in phage capsid. Then, their resistance to UV-C radiation and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) was compared to M13 wild-type vector (pC89) without peptide insert. Results showed that both the phage clones acquired an advantage against direct radiation damage, due to a reorganization of interactions in the capsid for an increase of H-bond and steric interactions. However, only P9b had an increase in resistance against H<sub>2</sub>O<sub>2</sub>. These results could help to understand the molecular mechanisms involved in the stability of new virus variants, also providing quick and necessary information to develop effective protocols in the virus inactivation for human activities, such as safety foods and animal-derived materials. |
| first_indexed | 2024-03-10T12:53:18Z |
| format | Article |
| id | doaj.art-74f72adc81624cccb5582b232bef2441 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T12:53:18Z |
| publishDate | 2021-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-74f72adc81624cccb5582b232bef24412023-11-21T12:06:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-03-01227340810.3390/ijms22073408Role of Phage Capsid in the Resistance to UV-C RadiationsLaura Maria De Plano0Domenico Franco1Maria Giovanna Rizzo2Vincenzo Zammuto3Concetta Gugliandolo4Letteria Silipigni5Lorenzo Torrisi6Salvatore P. P. Guglielmino7Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, ItalyDepartment of Mathematical and Computational Sciences, Physical Sciences and Earth Sciences, University of Messina, 98166 Messina, ItalyDepartment of Mathematical and Computational Sciences, Physical Sciences and Earth Sciences, University of Messina, 98166 Messina, ItalyDepartment of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98166 Messina, ItalyThe conformational variation of the viral capsid structure plays an essential role both for the environmental resistance and acid nuclear release during cellular infection. The aim of this study was to evaluate how capsid rearrangement in engineered phages of M13 protects viral DNA and peptide bonds from damage induced by UV-C radiation. From in silico 3D modelling analysis, two M13 engineered phage clones, namely P9b and 12III1, were chosen for (i) chemical features of amino acids sequences, (ii) rearrangements in the secondary structure of their pVIII proteins and (iii) in turn the interactions involved in phage capsid. Then, their resistance to UV-C radiation and hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) was compared to M13 wild-type vector (pC89) without peptide insert. Results showed that both the phage clones acquired an advantage against direct radiation damage, due to a reorganization of interactions in the capsid for an increase of H-bond and steric interactions. However, only P9b had an increase in resistance against H<sub>2</sub>O<sub>2</sub>. These results could help to understand the molecular mechanisms involved in the stability of new virus variants, also providing quick and necessary information to develop effective protocols in the virus inactivation for human activities, such as safety foods and animal-derived materials.https://www.mdpi.com/1422-0067/22/7/3408M13 engineered phageUV-Cionizing direct and indirect damagevirus stability |
| spellingShingle | Laura Maria De Plano Domenico Franco Maria Giovanna Rizzo Vincenzo Zammuto Concetta Gugliandolo Letteria Silipigni Lorenzo Torrisi Salvatore P. P. Guglielmino Role of Phage Capsid in the Resistance to UV-C Radiations International Journal of Molecular Sciences M13 engineered phage UV-C ionizing direct and indirect damage virus stability |
| title | Role of Phage Capsid in the Resistance to UV-C Radiations |
| title_full | Role of Phage Capsid in the Resistance to UV-C Radiations |
| title_fullStr | Role of Phage Capsid in the Resistance to UV-C Radiations |
| title_full_unstemmed | Role of Phage Capsid in the Resistance to UV-C Radiations |
| title_short | Role of Phage Capsid in the Resistance to UV-C Radiations |
| title_sort | role of phage capsid in the resistance to uv c radiations |
| topic | M13 engineered phage UV-C ionizing direct and indirect damage virus stability |
| url | https://www.mdpi.com/1422-0067/22/7/3408 |
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