Drought-induced proline is mainly synthesized in leaves and transported to roots in watermelon under water deficit
Proline accumulation has been shown to occur in plants in response to various environmental stresses. Although proline metabolism-related genes have been functionally characterized, the inter-organ transport of proline in stressed plants remains unclear. In this study, free proline was detected with...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2022-09-01
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| Series: | Horticultural Plant Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2468014122000620 |
| _version_ | 1798002222937669632 |
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| author | Zhongyuan Wang Yongchao Yang Vivek Yadav Wei Zhao Yaping He Xian Zhang Chunhua Wei |
| author_facet | Zhongyuan Wang Yongchao Yang Vivek Yadav Wei Zhao Yaping He Xian Zhang Chunhua Wei |
| author_sort | Zhongyuan Wang |
| collection | DOAJ |
| description | Proline accumulation has been shown to occur in plants in response to various environmental stresses. Although proline metabolism-related genes have been functionally characterized, the inter-organ transport of proline in stressed plants remains unclear. In this study, free proline was detected with significant accumulations in the roots, stems, and leaves of watermelon drought-tolerant germplasm M08 and drought-susceptible line Y34 under drought stress. Expression profiling and enzyme activity measurements revealed that ClP5CS1 gene, rather than its paralog ClP5CS2, mainly contributes to the proline synthesis in leaves via the Glu pathway. Additionally, over-expression of the ClP5CS genes significantly enhanced the drought tolerance of transgenic Arabidopsis lines. Furthermore, we confirmed that proline is mainly synthesized in leaves and transported to roots in watermelon under drought stress. Transcriptome and expression analyses revealed that the genes involved in proline metabolism exhibited different expression levels. Specifically, ClP5CS1 was upregulated in leaves and roots, while ClP5CS2 was downregulated under drought stress. Also, 415 and 362 differently expressed TFs were identified in roots and leaves, respectively, with the majority upregulated in the former. Ultimately, a model for proline metabolism was proposed. The findings of this study provided new insights into the biosynthesis, transport, and regulatory mechanism of drought-induced proline in plants. |
| first_indexed | 2024-04-11T11:48:43Z |
| format | Article |
| id | doaj.art-74f93d044fee48e1bd624e6084d82893 |
| institution | Directory Open Access Journal |
| issn | 2468-0141 |
| language | English |
| last_indexed | 2024-04-11T11:48:43Z |
| publishDate | 2022-09-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Horticultural Plant Journal |
| spelling | doaj.art-74f93d044fee48e1bd624e6084d828932022-12-22T04:25:27ZengKeAi Communications Co., Ltd.Horticultural Plant Journal2468-01412022-09-0185615626Drought-induced proline is mainly synthesized in leaves and transported to roots in watermelon under water deficitZhongyuan Wang0Yongchao Yang1Vivek Yadav2Wei Zhao3Yaping He4Xian Zhang5Chunhua Wei6College of Horticulture, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling 712100, Shaanxi, ChinaCollege of Horticulture, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling 712100, Shaanxi, China; College of Biological and Agricultural Sciences, Honghe University, Mengzi 661100, Yunnan, ChinaCollege of Horticulture, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling 712100, Shaanxi, ChinaCollege of Horticulture, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling 712100, Shaanxi, ChinaCollege of Horticulture, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling 712100, Shaanxi, ChinaCollege of Horticulture, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling 712100, Shaanxi, China; State Key Laboratory of Vegetable Germplasm Innovation, Tianjin Kernel Agricultural Corporation Ltd, Tianjin 300384, ChinaCollege of Horticulture, State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A & F University, Yangling 712100, Shaanxi, China; Corresponding author.Proline accumulation has been shown to occur in plants in response to various environmental stresses. Although proline metabolism-related genes have been functionally characterized, the inter-organ transport of proline in stressed plants remains unclear. In this study, free proline was detected with significant accumulations in the roots, stems, and leaves of watermelon drought-tolerant germplasm M08 and drought-susceptible line Y34 under drought stress. Expression profiling and enzyme activity measurements revealed that ClP5CS1 gene, rather than its paralog ClP5CS2, mainly contributes to the proline synthesis in leaves via the Glu pathway. Additionally, over-expression of the ClP5CS genes significantly enhanced the drought tolerance of transgenic Arabidopsis lines. Furthermore, we confirmed that proline is mainly synthesized in leaves and transported to roots in watermelon under drought stress. Transcriptome and expression analyses revealed that the genes involved in proline metabolism exhibited different expression levels. Specifically, ClP5CS1 was upregulated in leaves and roots, while ClP5CS2 was downregulated under drought stress. Also, 415 and 362 differently expressed TFs were identified in roots and leaves, respectively, with the majority upregulated in the former. Ultimately, a model for proline metabolism was proposed. The findings of this study provided new insights into the biosynthesis, transport, and regulatory mechanism of drought-induced proline in plants.http://www.sciencedirect.com/science/article/pii/S2468014122000620WatermelonDrought stressProlineInter-organ transport |
| spellingShingle | Zhongyuan Wang Yongchao Yang Vivek Yadav Wei Zhao Yaping He Xian Zhang Chunhua Wei Drought-induced proline is mainly synthesized in leaves and transported to roots in watermelon under water deficit Horticultural Plant Journal Watermelon Drought stress Proline Inter-organ transport |
| title | Drought-induced proline is mainly synthesized in leaves and transported to roots in watermelon under water deficit |
| title_full | Drought-induced proline is mainly synthesized in leaves and transported to roots in watermelon under water deficit |
| title_fullStr | Drought-induced proline is mainly synthesized in leaves and transported to roots in watermelon under water deficit |
| title_full_unstemmed | Drought-induced proline is mainly synthesized in leaves and transported to roots in watermelon under water deficit |
| title_short | Drought-induced proline is mainly synthesized in leaves and transported to roots in watermelon under water deficit |
| title_sort | drought induced proline is mainly synthesized in leaves and transported to roots in watermelon under water deficit |
| topic | Watermelon Drought stress Proline Inter-organ transport |
| url | http://www.sciencedirect.com/science/article/pii/S2468014122000620 |
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