Core Collection Formation in Guatemalan Wild Avocado Germplasm with Phenotypic and SSR Data
Guatemala’s wild avocado germplasm holds vital genetic value, but lacking conservation strategies imperils it. Studying its diversity is pivotal for conservation and breeding. The study aimed to comprehensively assess the wild avocado germplasm in Guatemala by combining phenotypic and genotypic data...
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MDPI AG
2023-09-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/9/2385 |
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| author | José Alejandro Ruiz-Chután Marie Kalousová Anna Maňourová Hewan Demissie Degu Julio Ernesto Berdúo-Sandoval Carlos Enrique Villanueva-González Bohdan Lojka |
| author_facet | José Alejandro Ruiz-Chután Marie Kalousová Anna Maňourová Hewan Demissie Degu Julio Ernesto Berdúo-Sandoval Carlos Enrique Villanueva-González Bohdan Lojka |
| author_sort | José Alejandro Ruiz-Chután |
| collection | DOAJ |
| description | Guatemala’s wild avocado germplasm holds vital genetic value, but lacking conservation strategies imperils it. Studying its diversity is pivotal for conservation and breeding. The study aimed to comprehensively assess the wild avocado germplasm in Guatemala by combining phenotypic and genotypic data and to create a core collection for conservation and future breeding programs. A total of 189 mature avocado trees were sampled across Guatemala’s northern, southern, and western regions. Morphological characteristics were documented, and genetic diversity was assessed using 12 SSR loci. The investigated germplasm revealed three distinct genetic clusters, exhibiting an average gene diversity of 0.796 and a 7.74% molecular variation among them. The samples showed various morphological characteristics that indicate the presence of three avocado races in Guatemala. The weak correlation between phenotypic and genotypic distances highlighted their independence and complementary nature. The joint matrix effectively integrated and captured genotypic and phenotypic data for comprehensive genetic diversity analysis. A core collection comprising 20% of total accessions that captured maximum genetic diversity was formed. This study exposed wild Guatemalan avocados’ genetic diversity, morphological traits, and conservation significance. Integrated data capture via clustering validates holistic genetic insight for conservation and breeding strategies. |
| first_indexed | 2024-03-10T23:07:32Z |
| format | Article |
| id | doaj.art-74fa3fde05aa429cb68e691ef680d42f |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T23:07:32Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-74fa3fde05aa429cb68e691ef680d42f2023-11-19T09:11:32ZengMDPI AGAgronomy2073-43952023-09-01139238510.3390/agronomy13092385Core Collection Formation in Guatemalan Wild Avocado Germplasm with Phenotypic and SSR DataJosé Alejandro Ruiz-Chután0Marie Kalousová1Anna Maňourová2Hewan Demissie Degu3Julio Ernesto Berdúo-Sandoval4Carlos Enrique Villanueva-González5Bohdan Lojka6Department of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech RepublicDepartment of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech RepublicDepartment of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech RepublicSchool of Plant and Horticulture Science, College of Agriculture, Hawassa University, Hawassa P.O. Box 05, EthiopiaFacultad de Agronomía, Universidad de San Carlos de Guatemala, Guatemala City 010012, GuatemalaDepartment of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech RepublicDepartment of Crop Sciences and Agroforestry, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Kamýcká 129, 165 00 Prague, Czech RepublicGuatemala’s wild avocado germplasm holds vital genetic value, but lacking conservation strategies imperils it. Studying its diversity is pivotal for conservation and breeding. The study aimed to comprehensively assess the wild avocado germplasm in Guatemala by combining phenotypic and genotypic data and to create a core collection for conservation and future breeding programs. A total of 189 mature avocado trees were sampled across Guatemala’s northern, southern, and western regions. Morphological characteristics were documented, and genetic diversity was assessed using 12 SSR loci. The investigated germplasm revealed three distinct genetic clusters, exhibiting an average gene diversity of 0.796 and a 7.74% molecular variation among them. The samples showed various morphological characteristics that indicate the presence of three avocado races in Guatemala. The weak correlation between phenotypic and genotypic distances highlighted their independence and complementary nature. The joint matrix effectively integrated and captured genotypic and phenotypic data for comprehensive genetic diversity analysis. A core collection comprising 20% of total accessions that captured maximum genetic diversity was formed. This study exposed wild Guatemalan avocados’ genetic diversity, morphological traits, and conservation significance. Integrated data capture via clustering validates holistic genetic insight for conservation and breeding strategies.https://www.mdpi.com/2073-4395/13/9/2385<i>Persea americana</i>clustering analysisallelic richnessgenetic resources conservationgenetic-phenotypic joint analysis |
| spellingShingle | José Alejandro Ruiz-Chután Marie Kalousová Anna Maňourová Hewan Demissie Degu Julio Ernesto Berdúo-Sandoval Carlos Enrique Villanueva-González Bohdan Lojka Core Collection Formation in Guatemalan Wild Avocado Germplasm with Phenotypic and SSR Data Agronomy <i>Persea americana</i> clustering analysis allelic richness genetic resources conservation genetic-phenotypic joint analysis |
| title | Core Collection Formation in Guatemalan Wild Avocado Germplasm with Phenotypic and SSR Data |
| title_full | Core Collection Formation in Guatemalan Wild Avocado Germplasm with Phenotypic and SSR Data |
| title_fullStr | Core Collection Formation in Guatemalan Wild Avocado Germplasm with Phenotypic and SSR Data |
| title_full_unstemmed | Core Collection Formation in Guatemalan Wild Avocado Germplasm with Phenotypic and SSR Data |
| title_short | Core Collection Formation in Guatemalan Wild Avocado Germplasm with Phenotypic and SSR Data |
| title_sort | core collection formation in guatemalan wild avocado germplasm with phenotypic and ssr data |
| topic | <i>Persea americana</i> clustering analysis allelic richness genetic resources conservation genetic-phenotypic joint analysis |
| url | https://www.mdpi.com/2073-4395/13/9/2385 |
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