Identification and characterization of microRNAs in normal equine tissues by Next Generation Sequencing.
The role of microRNAs (miRNAs) as a post-transcriptional gene regulator has been elucidated in a broad range of organisms including domestic animals. Characterization of miRNAs in normal tissues is an important step to investigate the functions of miRNAs in various physiological and pathological con...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3973549?pdf=render |
| _version_ | 1811232907596922880 |
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| author | Myung-Chul Kim Seung-Woo Lee Doug-Young Ryu Feng-Ji Cui Jong Bhak Yongbaek Kim |
| author_facet | Myung-Chul Kim Seung-Woo Lee Doug-Young Ryu Feng-Ji Cui Jong Bhak Yongbaek Kim |
| author_sort | Myung-Chul Kim |
| collection | DOAJ |
| description | The role of microRNAs (miRNAs) as a post-transcriptional gene regulator has been elucidated in a broad range of organisms including domestic animals. Characterization of miRNAs in normal tissues is an important step to investigate the functions of miRNAs in various physiological and pathological conditions. Using Illumina Next Generation Sequencing (NGS) technology, we identified a total of 292 known and 329 novel miRNAs in normal horse tissues including skeletal muscle, colon and liver. Distinct sets of miRNAs were differentially expressed in a tissue-specific manner. The miRNA genes were distributed across all the chromosomes except chromosomes 29 and 31 in the horse reference genome. In some chromosomes, multiple miRNAs were clustered and considered to be polycistronic transcript. A base composition analysis showed that equine miRNAs had a higher frequency of A+U than G+C. Furthermore, U tended to be more frequent at the 5' end of miRNA sequences. This is the first experimental study that identifies and characterizes the global miRNA expression profile in normal horse tissues. The present study enriches the horse miRNA database and provides useful information for further research dissecting biological functions of miRNAs in horse. |
| first_indexed | 2024-04-12T11:11:21Z |
| format | Article |
| id | doaj.art-74fa8b42bd704db08ef0bb384d2e009d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T11:11:21Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-74fa8b42bd704db08ef0bb384d2e009d2022-12-22T03:35:36ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0194e9366210.1371/journal.pone.0093662Identification and characterization of microRNAs in normal equine tissues by Next Generation Sequencing.Myung-Chul KimSeung-Woo LeeDoug-Young RyuFeng-Ji CuiJong BhakYongbaek KimThe role of microRNAs (miRNAs) as a post-transcriptional gene regulator has been elucidated in a broad range of organisms including domestic animals. Characterization of miRNAs in normal tissues is an important step to investigate the functions of miRNAs in various physiological and pathological conditions. Using Illumina Next Generation Sequencing (NGS) technology, we identified a total of 292 known and 329 novel miRNAs in normal horse tissues including skeletal muscle, colon and liver. Distinct sets of miRNAs were differentially expressed in a tissue-specific manner. The miRNA genes were distributed across all the chromosomes except chromosomes 29 and 31 in the horse reference genome. In some chromosomes, multiple miRNAs were clustered and considered to be polycistronic transcript. A base composition analysis showed that equine miRNAs had a higher frequency of A+U than G+C. Furthermore, U tended to be more frequent at the 5' end of miRNA sequences. This is the first experimental study that identifies and characterizes the global miRNA expression profile in normal horse tissues. The present study enriches the horse miRNA database and provides useful information for further research dissecting biological functions of miRNAs in horse.http://europepmc.org/articles/PMC3973549?pdf=render |
| spellingShingle | Myung-Chul Kim Seung-Woo Lee Doug-Young Ryu Feng-Ji Cui Jong Bhak Yongbaek Kim Identification and characterization of microRNAs in normal equine tissues by Next Generation Sequencing. PLoS ONE |
| title | Identification and characterization of microRNAs in normal equine tissues by Next Generation Sequencing. |
| title_full | Identification and characterization of microRNAs in normal equine tissues by Next Generation Sequencing. |
| title_fullStr | Identification and characterization of microRNAs in normal equine tissues by Next Generation Sequencing. |
| title_full_unstemmed | Identification and characterization of microRNAs in normal equine tissues by Next Generation Sequencing. |
| title_short | Identification and characterization of microRNAs in normal equine tissues by Next Generation Sequencing. |
| title_sort | identification and characterization of micrornas in normal equine tissues by next generation sequencing |
| url | http://europepmc.org/articles/PMC3973549?pdf=render |
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