Functional Analysis of <i>StPHT1;7</i>, a <i>Solanum tuberosum</i> L. Phosphate Transporter Gene, in Growth and Drought Tolerance
<i>PHT1</i> (phosphate transporter 1) family genes play important roles in regulating plant growth and responding to stress. However, there has been little research on the role of the <i>PHT1</i> family in potatoes. In this study, using molecular and bioinformatic approaches,...
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MDPI AG
2020-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/10/1384 |
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| author | Minxuan Cao Hengzhi Liu Chao Zhang Dongdong Wang Xiaofang Liu Qin Chen |
| author_facet | Minxuan Cao Hengzhi Liu Chao Zhang Dongdong Wang Xiaofang Liu Qin Chen |
| author_sort | Minxuan Cao |
| collection | DOAJ |
| description | <i>PHT1</i> (phosphate transporter 1) family genes play important roles in regulating plant growth and responding to stress. However, there has been little research on the role of the <i>PHT1</i> family in potatoes. In this study, using molecular and bioinformatic approaches, 8 <i>PHT1</i> family genes were identified from the potato genome. <i>StPHT1;7</i> was highly expressed in the whole potato plants. The overexpression and silence vectors of <i>StPHT1;7</i> were constructed and transformed into the potato cultivar Desiree. Consequently, <i>StPHT1;7</i> overexpression (with a relative expression 2–7-fold that in the control) and silence lines (with a relative expression of 0.3%–1% that in the control) were obtained. Their growth vigor was ranked in the order overexpression line > wild type > silence line. In the absence of phosphorus, the root length of the overexpression line was approximately 2.6 times that of the wild type, while the root length of the silence line was approximately 0.6 times that of the wild type. Furthermore, their tolerance to drought stress was ranked as wild type > overexpression line > silence line. These results suggest that <i>StPHT1;7</i> affects growth and stress tolerance in potato plants. |
| first_indexed | 2024-03-10T15:32:30Z |
| format | Article |
| id | doaj.art-74fcabc5ab1846679bcacd1533c6de27 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T15:32:30Z |
| publishDate | 2020-10-01 |
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| spelling | doaj.art-74fcabc5ab1846679bcacd1533c6de272023-11-20T17:30:58ZengMDPI AGPlants2223-77472020-10-01910138410.3390/plants9101384Functional Analysis of <i>StPHT1;7</i>, a <i>Solanum tuberosum</i> L. Phosphate Transporter Gene, in Growth and Drought ToleranceMinxuan Cao0Hengzhi Liu1Chao Zhang2Dongdong Wang3Xiaofang Liu4Qin Chen5State Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianning 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianning 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianning 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Agronomy, Northwest A&F University, Xianning 712100, ChinaState Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Xianning 712100, ChinaCollege of Food Science and Engineering, Northwest A&F University, Xianning 712100, China<i>PHT1</i> (phosphate transporter 1) family genes play important roles in regulating plant growth and responding to stress. However, there has been little research on the role of the <i>PHT1</i> family in potatoes. In this study, using molecular and bioinformatic approaches, 8 <i>PHT1</i> family genes were identified from the potato genome. <i>StPHT1;7</i> was highly expressed in the whole potato plants. The overexpression and silence vectors of <i>StPHT1;7</i> were constructed and transformed into the potato cultivar Desiree. Consequently, <i>StPHT1;7</i> overexpression (with a relative expression 2–7-fold that in the control) and silence lines (with a relative expression of 0.3%–1% that in the control) were obtained. Their growth vigor was ranked in the order overexpression line > wild type > silence line. In the absence of phosphorus, the root length of the overexpression line was approximately 2.6 times that of the wild type, while the root length of the silence line was approximately 0.6 times that of the wild type. Furthermore, their tolerance to drought stress was ranked as wild type > overexpression line > silence line. These results suggest that <i>StPHT1;7</i> affects growth and stress tolerance in potato plants.https://www.mdpi.com/2223-7747/9/10/1384potato<i>StPHT17</i>genetic transformationphosphorus stressdrought stress |
| spellingShingle | Minxuan Cao Hengzhi Liu Chao Zhang Dongdong Wang Xiaofang Liu Qin Chen Functional Analysis of <i>StPHT1;7</i>, a <i>Solanum tuberosum</i> L. Phosphate Transporter Gene, in Growth and Drought Tolerance Plants potato <i>StPHT1 7</i> genetic transformation phosphorus stress drought stress |
| title | Functional Analysis of <i>StPHT1;7</i>, a <i>Solanum tuberosum</i> L. Phosphate Transporter Gene, in Growth and Drought Tolerance |
| title_full | Functional Analysis of <i>StPHT1;7</i>, a <i>Solanum tuberosum</i> L. Phosphate Transporter Gene, in Growth and Drought Tolerance |
| title_fullStr | Functional Analysis of <i>StPHT1;7</i>, a <i>Solanum tuberosum</i> L. Phosphate Transporter Gene, in Growth and Drought Tolerance |
| title_full_unstemmed | Functional Analysis of <i>StPHT1;7</i>, a <i>Solanum tuberosum</i> L. Phosphate Transporter Gene, in Growth and Drought Tolerance |
| title_short | Functional Analysis of <i>StPHT1;7</i>, a <i>Solanum tuberosum</i> L. Phosphate Transporter Gene, in Growth and Drought Tolerance |
| title_sort | functional analysis of i stpht1 7 i a i solanum tuberosum i l phosphate transporter gene in growth and drought tolerance |
| topic | potato <i>StPHT1 7</i> genetic transformation phosphorus stress drought stress |
| url | https://www.mdpi.com/2223-7747/9/10/1384 |
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