Stem Bark-Mediated Green Synthesis of Silver Nanoparticles from <i>Pyrus pashia</i>: Characterization, Antioxidant, and Antibacterial Properties
The investigation of using medicinal plants for the production and application of silver nanoparticles (AgNPs) has attracted growing research interest. In this study, AgNPs are synthesized from the stem barks of the <i>Pyrus pashia</i> medicinal plant using a biosynthetic strategy. The r...
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MDPI AG
2023-06-01
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| Series: | Inorganics |
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| Online Access: | https://www.mdpi.com/2304-6740/11/6/263 |
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| author | Lekha Nath Khanal Purna Prasad Dhakal Mani Ram Kandel Debendra Acharya Ek Raj Baral Kisan Chhetri Surya Kant Kalauni |
| author_facet | Lekha Nath Khanal Purna Prasad Dhakal Mani Ram Kandel Debendra Acharya Ek Raj Baral Kisan Chhetri Surya Kant Kalauni |
| author_sort | Lekha Nath Khanal |
| collection | DOAJ |
| description | The investigation of using medicinal plants for the production and application of silver nanoparticles (AgNPs) has attracted growing research interest. In this study, AgNPs are synthesized from the stem barks of the <i>Pyrus pashia</i> medicinal plant using a biosynthetic strategy. The reaction conditions were optimized under ambient conditions, including concentration, temperature, time, and pH, and various techniques were employed, such as UV-visible, FTIR, XRD, FESEM, and TEM, to characterize the synthesized AgNPs. The AgNPs produced through this biosynthesis method were found to be spherical and polydispersed, with an average size of 23.92 ± 7.04 nm. The synthesized AgNPs demonstrated an enhanced DPPH free radical scavenging capacity compared to the aqueous extract, with IC<sub>50</sub> values of 10.67 ± 0.05 µg/mL and 13.66 ± 0.35 µg/mL, respectively. In the agar well diffusion method, the synthesized AgNPs showed higher antibacterial activity than that of the extract against <i>Escherichia coli</i> (ATCC 25922), <i>Staphylococcus aureus</i> (ATCC 25923), <i>Enterococcus faecalis</i> (ATCC 29212), <i>Salmonella typhi</i> (ATCC 14028), and <i>Shigella sonnei</i> (ATCC 25931). Based on these findings, the study suggests that green synthesized AgNPs from <i>P. pashia</i> could be used for biomedical applications. |
| first_indexed | 2024-03-11T02:19:27Z |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-03-11T02:19:27Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Inorganics |
| spelling | doaj.art-74ffe5693dd74d7198ea042478311c9a2023-11-18T10:55:31ZengMDPI AGInorganics2304-67402023-06-0111626310.3390/inorganics11060263Stem Bark-Mediated Green Synthesis of Silver Nanoparticles from <i>Pyrus pashia</i>: Characterization, Antioxidant, and Antibacterial PropertiesLekha Nath Khanal0Purna Prasad Dhakal1Mani Ram Kandel2Debendra Acharya3Ek Raj Baral4Kisan Chhetri5Surya Kant Kalauni6Department of Chemistry, Prithvi Narayan Campus, Tribhuvan University, Pokhara 33700, NepalCentral Department of Chemistry, Tribhuvan University, Kathmandu 44613, NepalDepartment of Chemistry, Amrit Campus, Tribhuvan University, Kathmandu 44613, NepalDepartment of Nano Convergence Engineering, Jeonbuk National University, Jeonju 54896, Republic of KoreaDepartment of Chemistry, Research Institute of Physics and Chemistry, Jeonbuk National University, Jeonju 54896, Republic of KoreaDepartment of Nano Convergence Engineering, Jeonbuk National University, Jeonju 54896, Republic of KoreaCentral Department of Chemistry, Tribhuvan University, Kathmandu 44613, NepalThe investigation of using medicinal plants for the production and application of silver nanoparticles (AgNPs) has attracted growing research interest. In this study, AgNPs are synthesized from the stem barks of the <i>Pyrus pashia</i> medicinal plant using a biosynthetic strategy. The reaction conditions were optimized under ambient conditions, including concentration, temperature, time, and pH, and various techniques were employed, such as UV-visible, FTIR, XRD, FESEM, and TEM, to characterize the synthesized AgNPs. The AgNPs produced through this biosynthesis method were found to be spherical and polydispersed, with an average size of 23.92 ± 7.04 nm. The synthesized AgNPs demonstrated an enhanced DPPH free radical scavenging capacity compared to the aqueous extract, with IC<sub>50</sub> values of 10.67 ± 0.05 µg/mL and 13.66 ± 0.35 µg/mL, respectively. In the agar well diffusion method, the synthesized AgNPs showed higher antibacterial activity than that of the extract against <i>Escherichia coli</i> (ATCC 25922), <i>Staphylococcus aureus</i> (ATCC 25923), <i>Enterococcus faecalis</i> (ATCC 29212), <i>Salmonella typhi</i> (ATCC 14028), and <i>Shigella sonnei</i> (ATCC 25931). Based on these findings, the study suggests that green synthesized AgNPs from <i>P. pashia</i> could be used for biomedical applications.https://www.mdpi.com/2304-6740/11/6/263green synthesis<i>Pyrus pashia</i>silver nanoparticlesantioxidant activityantibacterial activity |
| spellingShingle | Lekha Nath Khanal Purna Prasad Dhakal Mani Ram Kandel Debendra Acharya Ek Raj Baral Kisan Chhetri Surya Kant Kalauni Stem Bark-Mediated Green Synthesis of Silver Nanoparticles from <i>Pyrus pashia</i>: Characterization, Antioxidant, and Antibacterial Properties Inorganics green synthesis <i>Pyrus pashia</i> silver nanoparticles antioxidant activity antibacterial activity |
| title | Stem Bark-Mediated Green Synthesis of Silver Nanoparticles from <i>Pyrus pashia</i>: Characterization, Antioxidant, and Antibacterial Properties |
| title_full | Stem Bark-Mediated Green Synthesis of Silver Nanoparticles from <i>Pyrus pashia</i>: Characterization, Antioxidant, and Antibacterial Properties |
| title_fullStr | Stem Bark-Mediated Green Synthesis of Silver Nanoparticles from <i>Pyrus pashia</i>: Characterization, Antioxidant, and Antibacterial Properties |
| title_full_unstemmed | Stem Bark-Mediated Green Synthesis of Silver Nanoparticles from <i>Pyrus pashia</i>: Characterization, Antioxidant, and Antibacterial Properties |
| title_short | Stem Bark-Mediated Green Synthesis of Silver Nanoparticles from <i>Pyrus pashia</i>: Characterization, Antioxidant, and Antibacterial Properties |
| title_sort | stem bark mediated green synthesis of silver nanoparticles from i pyrus pashia i characterization antioxidant and antibacterial properties |
| topic | green synthesis <i>Pyrus pashia</i> silver nanoparticles antioxidant activity antibacterial activity |
| url | https://www.mdpi.com/2304-6740/11/6/263 |
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