Online Biomass Monitoring Enables Characterization of the Growth Pattern of <i>Aspergillus fumigatus</i> in Liquid Shake Conditions
Numerous filamentous fungal species are extensively studied due to their role as model organisms, workhorses in biotechnology, or as pathogens for plants, animals, and humans. Growth studies are mainly carried out on solid media. However, studies concerning gene expression, biochemistry, or metaboli...
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| Format: | Article |
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MDPI AG
2022-09-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/10/1013 |
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| author | Ingo Bauer Beate Abt Annie Yap Bernd Leuchtle Hubertus Haas |
| author_facet | Ingo Bauer Beate Abt Annie Yap Bernd Leuchtle Hubertus Haas |
| author_sort | Ingo Bauer |
| collection | DOAJ |
| description | Numerous filamentous fungal species are extensively studied due to their role as model organisms, workhorses in biotechnology, or as pathogens for plants, animals, and humans. Growth studies are mainly carried out on solid media. However, studies concerning gene expression, biochemistry, or metabolism are carried out usually in liquid shake conditions, which do not correspond to the growth pattern on solid media. The reason for this practice is the problem of on-line growth monitoring of filamentous fungal species, which usually form pellets in liquid shake cultures. Here, we compared the time-consuming and tedious process of dry-weight determination of the mold <i>Aspergillus fumigatus</i> with online monitoring of biomass in liquid shake culture by the parallelizable CGQ (“cell growth quantifier”), which implements dynamic biomass determination by backscattered light measurement. The results revealed a strong correlation of CGQ-mediated growth monitoring and classical biomass measurement of <i>A. fumigatus</i> grown over a time course. Moreover, CGQ-mediated growth monitoring displayed the difference in growth of <i>A. fumigatus</i> in response to the limitation of iron or nitrogen as well as the growth defects of previously reported mutant strains (Δ<i>hapX,</i> Δ<i>srbA</i>). Furthermore, the frequently used wild-type strain Af293 showed largely decreased and delayed growth in liquid shake cultures compared to other strains (AfS77, A1160p+, AfS35). Taken together, the CGQ allows for robust, automated biomass monitoring of <i>A. fumigatus</i> during liquid shake conditions, which largely facilitates the characterization of the growth pattern of filamentous fungal species. |
| first_indexed | 2024-03-09T20:00:04Z |
| format | Article |
| id | doaj.art-750308df5c2d4b28a5788c63d570527f |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T20:00:04Z |
| publishDate | 2022-09-01 |
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| series | Journal of Fungi |
| spelling | doaj.art-750308df5c2d4b28a5788c63d570527f2023-11-24T00:46:40ZengMDPI AGJournal of Fungi2309-608X2022-09-01810101310.3390/jof8101013Online Biomass Monitoring Enables Characterization of the Growth Pattern of <i>Aspergillus fumigatus</i> in Liquid Shake ConditionsIngo Bauer0Beate Abt1Annie Yap2Bernd Leuchtle3Hubertus Haas4Institute of Molecular Biology, Biocenter, Medical University of Innsbruck, Innrain 80-82, 6020 Innsbruck, AustriaInstitute of Molecular Biology, Biocenter, Medical University of Innsbruck, Innrain 80-82, 6020 Innsbruck, AustriaInstitute of Molecular Biology, Biocenter, Medical University of Innsbruck, Innrain 80-82, 6020 Innsbruck, AustriaSBI, Scientific Bioprocessing, 520 William Pitt Way, Pittsburgh, PA 15238, USAInstitute of Molecular Biology, Biocenter, Medical University of Innsbruck, Innrain 80-82, 6020 Innsbruck, AustriaNumerous filamentous fungal species are extensively studied due to their role as model organisms, workhorses in biotechnology, or as pathogens for plants, animals, and humans. Growth studies are mainly carried out on solid media. However, studies concerning gene expression, biochemistry, or metabolism are carried out usually in liquid shake conditions, which do not correspond to the growth pattern on solid media. The reason for this practice is the problem of on-line growth monitoring of filamentous fungal species, which usually form pellets in liquid shake cultures. Here, we compared the time-consuming and tedious process of dry-weight determination of the mold <i>Aspergillus fumigatus</i> with online monitoring of biomass in liquid shake culture by the parallelizable CGQ (“cell growth quantifier”), which implements dynamic biomass determination by backscattered light measurement. The results revealed a strong correlation of CGQ-mediated growth monitoring and classical biomass measurement of <i>A. fumigatus</i> grown over a time course. Moreover, CGQ-mediated growth monitoring displayed the difference in growth of <i>A. fumigatus</i> in response to the limitation of iron or nitrogen as well as the growth defects of previously reported mutant strains (Δ<i>hapX,</i> Δ<i>srbA</i>). Furthermore, the frequently used wild-type strain Af293 showed largely decreased and delayed growth in liquid shake cultures compared to other strains (AfS77, A1160p+, AfS35). Taken together, the CGQ allows for robust, automated biomass monitoring of <i>A. fumigatus</i> during liquid shake conditions, which largely facilitates the characterization of the growth pattern of filamentous fungal species.https://www.mdpi.com/2309-608X/8/10/1013fungimolds<i>Aspergillus fumigatus</i>liquid shake cultureflask cultureonline monitoring |
| spellingShingle | Ingo Bauer Beate Abt Annie Yap Bernd Leuchtle Hubertus Haas Online Biomass Monitoring Enables Characterization of the Growth Pattern of <i>Aspergillus fumigatus</i> in Liquid Shake Conditions Journal of Fungi fungi molds <i>Aspergillus fumigatus</i> liquid shake culture flask culture online monitoring |
| title | Online Biomass Monitoring Enables Characterization of the Growth Pattern of <i>Aspergillus fumigatus</i> in Liquid Shake Conditions |
| title_full | Online Biomass Monitoring Enables Characterization of the Growth Pattern of <i>Aspergillus fumigatus</i> in Liquid Shake Conditions |
| title_fullStr | Online Biomass Monitoring Enables Characterization of the Growth Pattern of <i>Aspergillus fumigatus</i> in Liquid Shake Conditions |
| title_full_unstemmed | Online Biomass Monitoring Enables Characterization of the Growth Pattern of <i>Aspergillus fumigatus</i> in Liquid Shake Conditions |
| title_short | Online Biomass Monitoring Enables Characterization of the Growth Pattern of <i>Aspergillus fumigatus</i> in Liquid Shake Conditions |
| title_sort | online biomass monitoring enables characterization of the growth pattern of i aspergillus fumigatus i in liquid shake conditions |
| topic | fungi molds <i>Aspergillus fumigatus</i> liquid shake culture flask culture online monitoring |
| url | https://www.mdpi.com/2309-608X/8/10/1013 |
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