Pericellular collagen I coating for enhanced homing and chondrogenic differentiation of mesenchymal stem cells in direct intra-articular injection
Abstract Background Direct intra-articular injection (DIAI) of mesenchymal stem cells (MSCs) is a promising technique for cartilage repair. However, the repair process was hindered by the absence of scaffold and poor cell–matrix interactions. Methods In this study, we developed a pericellular collag...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2018-06-01
|
| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13287-018-0916-z |
| _version_ | 1818591567080849408 |
|---|---|
| author | Hansong Xia Chi Liang Pan Luo Junjie Huang Jinshen He Zili Wang Xu Cao Cheng Peng Song Wu |
| author_facet | Hansong Xia Chi Liang Pan Luo Junjie Huang Jinshen He Zili Wang Xu Cao Cheng Peng Song Wu |
| author_sort | Hansong Xia |
| collection | DOAJ |
| description | Abstract Background Direct intra-articular injection (DIAI) of mesenchymal stem cells (MSCs) is a promising technique for cartilage repair. However, the repair process was hindered by the absence of scaffold and poor cell–matrix interactions. Methods In this study, we developed a pericellular collagen I coating (PCC) on MSCs. The overall performances of MSC-PCC homing, chondrogenic differentiation, and cartilage regeneration have been comprehensively evaluated in a New Zealand rabbit model. Firstly, we examined the morphology and physical characteristics of PCC. Secondly, MSC ex-vivo cartilage slice adhesion and in-vivo cartilage defect homing were observed using multiscale methods. Thirdly, the precartilage condensation of cell pellets formed by aggregation of MSCs was examined to evaluate the cartilage-inducing potential of PCC. Finally, the cartilage regeneration by DIAI of PCC-coated MSCs was observed and scored macroscopically and histologically. Results In general, the cell adhesion and homing assay revealed that PCC facilitated MSC adhesion on cartilage slices, enhancing MSC homing and retention to cartilage defect. This increased homing ratio was accompanied by an increasing cell–cell contact. Compared with naked MSCs, the cell pellets formed by PCC-coated MSCs exhibited more evident appearance of condensation. In pellets, cell–cell interaction has been significantly stimulated, inducing the expression of condensation marker N-cadherin, and subsequent chondrogenic marker collagen II and aggrecan. By 12 weeks after DIAI, cartilage defects have been repaired by MSCs to varying degrees. Overall, PCC significantly enhances the quality of cartilage regeneration judging from macroscopic observation, ICRS score, histological examination, and collagen type I, II, and X immunohistochemical staining. Conclusions The capacity and viability of MSCs can be enhanced by collagen I coating, which provides cues for enhancing cell homing and differentiation. Our method provides a novel strategy for stem cell therapy. |
| first_indexed | 2024-12-16T10:14:29Z |
| format | Article |
| id | doaj.art-750636431a7a4a88a85585866eec9bac |
| institution | Directory Open Access Journal |
| issn | 1757-6512 |
| language | English |
| last_indexed | 2024-12-16T10:14:29Z |
| publishDate | 2018-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj.art-750636431a7a4a88a85585866eec9bac2022-12-21T22:35:29ZengBMCStem Cell Research & Therapy1757-65122018-06-019111210.1186/s13287-018-0916-zPericellular collagen I coating for enhanced homing and chondrogenic differentiation of mesenchymal stem cells in direct intra-articular injectionHansong Xia0Chi Liang1Pan Luo2Junjie Huang3Jinshen He4Zili Wang5Xu Cao6Cheng Peng7Song Wu8Department of Orthopaedics, 3rd Xiangya Hospital, Central South UniversityDepartment of Orthopaedics, 3rd Xiangya Hospital, Central South UniversityDepartment of Orthopaedics, 3rd Xiangya Hospital, Central South UniversityDepartment of Orthopaedics, 3rd Xiangya Hospital, Central South UniversityDepartment of Orthopaedics, 3rd Xiangya Hospital, Central South UniversityDepartment of Orthopaedics, 3rd Xiangya Hospital, Central South UniversityDepartment of Orthopaedics, 3rd Xiangya Hospital, Central South UniversityDepartment of Burns and Plastic Surgery, 3rd Xiangya Hospital, Central South UniversityDepartment of Orthopaedics, 3rd Xiangya Hospital, Central South UniversityAbstract Background Direct intra-articular injection (DIAI) of mesenchymal stem cells (MSCs) is a promising technique for cartilage repair. However, the repair process was hindered by the absence of scaffold and poor cell–matrix interactions. Methods In this study, we developed a pericellular collagen I coating (PCC) on MSCs. The overall performances of MSC-PCC homing, chondrogenic differentiation, and cartilage regeneration have been comprehensively evaluated in a New Zealand rabbit model. Firstly, we examined the morphology and physical characteristics of PCC. Secondly, MSC ex-vivo cartilage slice adhesion and in-vivo cartilage defect homing were observed using multiscale methods. Thirdly, the precartilage condensation of cell pellets formed by aggregation of MSCs was examined to evaluate the cartilage-inducing potential of PCC. Finally, the cartilage regeneration by DIAI of PCC-coated MSCs was observed and scored macroscopically and histologically. Results In general, the cell adhesion and homing assay revealed that PCC facilitated MSC adhesion on cartilage slices, enhancing MSC homing and retention to cartilage defect. This increased homing ratio was accompanied by an increasing cell–cell contact. Compared with naked MSCs, the cell pellets formed by PCC-coated MSCs exhibited more evident appearance of condensation. In pellets, cell–cell interaction has been significantly stimulated, inducing the expression of condensation marker N-cadherin, and subsequent chondrogenic marker collagen II and aggrecan. By 12 weeks after DIAI, cartilage defects have been repaired by MSCs to varying degrees. Overall, PCC significantly enhances the quality of cartilage regeneration judging from macroscopic observation, ICRS score, histological examination, and collagen type I, II, and X immunohistochemical staining. Conclusions The capacity and viability of MSCs can be enhanced by collagen I coating, which provides cues for enhancing cell homing and differentiation. Our method provides a novel strategy for stem cell therapy.http://link.springer.com/article/10.1186/s13287-018-0916-zCartilage injuryCartilage repairChondrogenic differentiationBone marrow mesenchymal stem cellsHoming |
| spellingShingle | Hansong Xia Chi Liang Pan Luo Junjie Huang Jinshen He Zili Wang Xu Cao Cheng Peng Song Wu Pericellular collagen I coating for enhanced homing and chondrogenic differentiation of mesenchymal stem cells in direct intra-articular injection Stem Cell Research & Therapy Cartilage injury Cartilage repair Chondrogenic differentiation Bone marrow mesenchymal stem cells Homing |
| title | Pericellular collagen I coating for enhanced homing and chondrogenic differentiation of mesenchymal stem cells in direct intra-articular injection |
| title_full | Pericellular collagen I coating for enhanced homing and chondrogenic differentiation of mesenchymal stem cells in direct intra-articular injection |
| title_fullStr | Pericellular collagen I coating for enhanced homing and chondrogenic differentiation of mesenchymal stem cells in direct intra-articular injection |
| title_full_unstemmed | Pericellular collagen I coating for enhanced homing and chondrogenic differentiation of mesenchymal stem cells in direct intra-articular injection |
| title_short | Pericellular collagen I coating for enhanced homing and chondrogenic differentiation of mesenchymal stem cells in direct intra-articular injection |
| title_sort | pericellular collagen i coating for enhanced homing and chondrogenic differentiation of mesenchymal stem cells in direct intra articular injection |
| topic | Cartilage injury Cartilage repair Chondrogenic differentiation Bone marrow mesenchymal stem cells Homing |
| url | http://link.springer.com/article/10.1186/s13287-018-0916-z |
| work_keys_str_mv | AT hansongxia pericellularcollagenicoatingforenhancedhomingandchondrogenicdifferentiationofmesenchymalstemcellsindirectintraarticularinjection AT chiliang pericellularcollagenicoatingforenhancedhomingandchondrogenicdifferentiationofmesenchymalstemcellsindirectintraarticularinjection AT panluo pericellularcollagenicoatingforenhancedhomingandchondrogenicdifferentiationofmesenchymalstemcellsindirectintraarticularinjection AT junjiehuang pericellularcollagenicoatingforenhancedhomingandchondrogenicdifferentiationofmesenchymalstemcellsindirectintraarticularinjection AT jinshenhe pericellularcollagenicoatingforenhancedhomingandchondrogenicdifferentiationofmesenchymalstemcellsindirectintraarticularinjection AT ziliwang pericellularcollagenicoatingforenhancedhomingandchondrogenicdifferentiationofmesenchymalstemcellsindirectintraarticularinjection AT xucao pericellularcollagenicoatingforenhancedhomingandchondrogenicdifferentiationofmesenchymalstemcellsindirectintraarticularinjection AT chengpeng pericellularcollagenicoatingforenhancedhomingandchondrogenicdifferentiationofmesenchymalstemcellsindirectintraarticularinjection AT songwu pericellularcollagenicoatingforenhancedhomingandchondrogenicdifferentiationofmesenchymalstemcellsindirectintraarticularinjection |