Use of Synchrotron Phase-Sensitive Imaging for the Investigation of Magnetopriming and Solar UV-Exclusion Impact on Soybean (<i>Glycine max</i>) Leaves
The combined response of exclusion of solar ultraviolet radiation (UV-A+B and UV-B) and static magnetic field (SMF) pre-treatment of 200 mT for 1 h were studied on soybean (<i>Glycine max</i>) leaves using synchrotron imaging. The seeds of soybean with and without SMF pre-treatment were...
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MDPI AG
2021-07-01
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| author | Anis Fatima Sunita Kataria Ashish Kumar Agrawal Balwant Singh Yogesh Kashyap Meeta Jain Marian Brestic Suleyman I. Allakhverdiev Anshu Rastogi |
| author_facet | Anis Fatima Sunita Kataria Ashish Kumar Agrawal Balwant Singh Yogesh Kashyap Meeta Jain Marian Brestic Suleyman I. Allakhverdiev Anshu Rastogi |
| author_sort | Anis Fatima |
| collection | DOAJ |
| description | The combined response of exclusion of solar ultraviolet radiation (UV-A+B and UV-B) and static magnetic field (SMF) pre-treatment of 200 mT for 1 h were studied on soybean (<i>Glycine max</i>) leaves using synchrotron imaging. The seeds of soybean with and without SMF pre-treatment were sown in nursery bags kept in iron meshes where UV-A+B (280–400 nm) and UV-B (280–315 nm) from solar radiation were filtered through a polyester filters. Two controls were planned, one with polythene filter controls (FC)- which allows all the UV (280–400 nm); the other control had no filter used (open control-OC). Midrib regions of the intact third trifoliate leaves were imaged using the phase-contrast imaging technique at BL-4, Indus-2 synchrotron radiation source. The solar UV exclusion results suggest that ambient UV caused a reduction in leaf growth which ultimately reduced the photosynthesis in soybean seedlings, while SMF treatment caused enhancement of leaf growth along with photosynthesis even under the presence of ambient UV-B stress. The width of midrib and second-order veins, length of the second-order veins, leaf vein density, and the density of third-order veins obtained from the quantitative image analysis showed an enhancement in the leaves of plants that emerged from SMF pre-treated seeds as compared to untreated ones grown in open control and filter control conditions (in the presence of ambient UV stress). SMF pre-treated seeds along with UV-A+B and UV-B exclusion also showed significant enhancements in leaf parameters as compared to the UV excluded untreated leaves. Our results suggested that SMF-pretreatment of seeds diminishes the ambient UV-induced adverse effects on soybean. |
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| last_indexed | 2024-03-10T09:43:11Z |
| publishDate | 2021-07-01 |
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| series | Cells |
| spelling | doaj.art-75066b13b51d41c6b106c099e50a58612023-11-22T03:29:02ZengMDPI AGCells2073-44092021-07-01107172510.3390/cells10071725Use of Synchrotron Phase-Sensitive Imaging for the Investigation of Magnetopriming and Solar UV-Exclusion Impact on Soybean (<i>Glycine max</i>) LeavesAnis Fatima0Sunita Kataria1Ashish Kumar Agrawal2Balwant Singh3Yogesh Kashyap4Meeta Jain5Marian Brestic6Suleyman I. Allakhverdiev7Anshu Rastogi8Technical Physics Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, IndiaSchool of Biochemistry, Devi Ahilya Vishwavidyalaya, Khandwa Road, Indore 452001, IndiaTechnical Physics Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, IndiaTechnical Physics Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, IndiaTechnical Physics Division, Bhabha Atomic Research Centre, Trombay, Mumbai 400085, IndiaSchool of Biochemistry, Devi Ahilya Vishwavidyalaya, Khandwa Road, Indore 452001, IndiaDepartment of Plant Physiology, Slovak University of Agriculture, A. Hlinku 2, 94976 Nitra, SlovakiaK.A. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya St. 35, 127276 Moscow, RussiaLaboratory of Bioclimatology, Department of Ecology and Environmental Protection, Poznan University of Life Sciences, Piątkowska 94, 60-649 Poznan, PolandThe combined response of exclusion of solar ultraviolet radiation (UV-A+B and UV-B) and static magnetic field (SMF) pre-treatment of 200 mT for 1 h were studied on soybean (<i>Glycine max</i>) leaves using synchrotron imaging. The seeds of soybean with and without SMF pre-treatment were sown in nursery bags kept in iron meshes where UV-A+B (280–400 nm) and UV-B (280–315 nm) from solar radiation were filtered through a polyester filters. Two controls were planned, one with polythene filter controls (FC)- which allows all the UV (280–400 nm); the other control had no filter used (open control-OC). Midrib regions of the intact third trifoliate leaves were imaged using the phase-contrast imaging technique at BL-4, Indus-2 synchrotron radiation source. The solar UV exclusion results suggest that ambient UV caused a reduction in leaf growth which ultimately reduced the photosynthesis in soybean seedlings, while SMF treatment caused enhancement of leaf growth along with photosynthesis even under the presence of ambient UV-B stress. The width of midrib and second-order veins, length of the second-order veins, leaf vein density, and the density of third-order veins obtained from the quantitative image analysis showed an enhancement in the leaves of plants that emerged from SMF pre-treated seeds as compared to untreated ones grown in open control and filter control conditions (in the presence of ambient UV stress). SMF pre-treated seeds along with UV-A+B and UV-B exclusion also showed significant enhancements in leaf parameters as compared to the UV excluded untreated leaves. Our results suggested that SMF-pretreatment of seeds diminishes the ambient UV-induced adverse effects on soybean.https://www.mdpi.com/2073-4409/10/7/1725phase-sensitive imagingmagnetoprimingUV exclusionleaf venationleaf hydraulics |
| spellingShingle | Anis Fatima Sunita Kataria Ashish Kumar Agrawal Balwant Singh Yogesh Kashyap Meeta Jain Marian Brestic Suleyman I. Allakhverdiev Anshu Rastogi Use of Synchrotron Phase-Sensitive Imaging for the Investigation of Magnetopriming and Solar UV-Exclusion Impact on Soybean (<i>Glycine max</i>) Leaves Cells phase-sensitive imaging magnetopriming UV exclusion leaf venation leaf hydraulics |
| title | Use of Synchrotron Phase-Sensitive Imaging for the Investigation of Magnetopriming and Solar UV-Exclusion Impact on Soybean (<i>Glycine max</i>) Leaves |
| title_full | Use of Synchrotron Phase-Sensitive Imaging for the Investigation of Magnetopriming and Solar UV-Exclusion Impact on Soybean (<i>Glycine max</i>) Leaves |
| title_fullStr | Use of Synchrotron Phase-Sensitive Imaging for the Investigation of Magnetopriming and Solar UV-Exclusion Impact on Soybean (<i>Glycine max</i>) Leaves |
| title_full_unstemmed | Use of Synchrotron Phase-Sensitive Imaging for the Investigation of Magnetopriming and Solar UV-Exclusion Impact on Soybean (<i>Glycine max</i>) Leaves |
| title_short | Use of Synchrotron Phase-Sensitive Imaging for the Investigation of Magnetopriming and Solar UV-Exclusion Impact on Soybean (<i>Glycine max</i>) Leaves |
| title_sort | use of synchrotron phase sensitive imaging for the investigation of magnetopriming and solar uv exclusion impact on soybean i glycine max i leaves |
| topic | phase-sensitive imaging magnetopriming UV exclusion leaf venation leaf hydraulics |
| url | https://www.mdpi.com/2073-4409/10/7/1725 |
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