Isolation and Characterization of Facultative-Anaerobic Antimonate-Reducing Bacteria
Microbial antimonate (Sb(V)) reduction is a promising approach to remove Sb(V) from wastewater. However, current knowledge regarding microbial Sb(V) reduction is limited to strictly anaerobic conditions. This study was the first to isolate three facultative-anaerobic Sb(V)-reducing bacterial strains...
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MDPI AG
2020-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/9/1435 |
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| author | Ziran Yang Hisaaki Hosokawa Takuya Sadakane Masashi Kuroda Daisuke Inoue Hiroshi Nishikawa Michihiko Ike |
| author_facet | Ziran Yang Hisaaki Hosokawa Takuya Sadakane Masashi Kuroda Daisuke Inoue Hiroshi Nishikawa Michihiko Ike |
| author_sort | Ziran Yang |
| collection | DOAJ |
| description | Microbial antimonate (Sb(V)) reduction is a promising approach to remove Sb(V) from wastewater. However, current knowledge regarding microbial Sb(V) reduction is limited to strictly anaerobic conditions. This study was the first to isolate three facultative-anaerobic Sb(V)-reducing bacterial strains from the sludge collected from a wastewater treatment facility in an antimony products plant. Two of the isolated strains, designated <i>Dechloromonas</i> sp. AR-2 and <i>Propionivibrio</i> sp. AR-3, were characterized based on their Sb(V)-reducing abilities. When cultivated under anaerobic conditions with Sb(V) and acetate as the electron acceptor and donor, respectively, both strains could efficiently reduce 5.0 mM Sb(V), removing most of it from the water phase within 7 d. Along with Sb(V) reduction by the strains, white precipitates, which were likely amorphous Sb(OH)<sub>3</sub> solids, were formed with a minor generation of soluble antimonite. Additionally, respiratory Sb(V) reduction by both strains occurred not only under anaerobic but also microaerobic conditions. It was suggested that Sb(V) reduction and the growth abilities of the strains under microaerobic conditions presented a substantial advantage of the use of strains AR-2 and AR-3 for practical applications to Sb(V)-containing wastewater treatment. |
| first_indexed | 2024-03-10T16:12:38Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T16:12:38Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-750b56031e974068b44f882e66ae20bb2023-11-20T14:18:23ZengMDPI AGMicroorganisms2076-26072020-09-0189143510.3390/microorganisms8091435Isolation and Characterization of Facultative-Anaerobic Antimonate-Reducing BacteriaZiran Yang0Hisaaki Hosokawa1Takuya Sadakane2Masashi Kuroda3Daisuke Inoue4Hiroshi Nishikawa5Michihiko Ike6Division of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanDivision of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanDivision of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanDivision of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanDivision of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanJoining and Welding Research Institute, Osaka University, 11-1 Mihogaoka, Ibaraki, Osaka 567-0047, JapanDivision of Sustainable Energy and Environmental Engineering, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, JapanMicrobial antimonate (Sb(V)) reduction is a promising approach to remove Sb(V) from wastewater. However, current knowledge regarding microbial Sb(V) reduction is limited to strictly anaerobic conditions. This study was the first to isolate three facultative-anaerobic Sb(V)-reducing bacterial strains from the sludge collected from a wastewater treatment facility in an antimony products plant. Two of the isolated strains, designated <i>Dechloromonas</i> sp. AR-2 and <i>Propionivibrio</i> sp. AR-3, were characterized based on their Sb(V)-reducing abilities. When cultivated under anaerobic conditions with Sb(V) and acetate as the electron acceptor and donor, respectively, both strains could efficiently reduce 5.0 mM Sb(V), removing most of it from the water phase within 7 d. Along with Sb(V) reduction by the strains, white precipitates, which were likely amorphous Sb(OH)<sub>3</sub> solids, were formed with a minor generation of soluble antimonite. Additionally, respiratory Sb(V) reduction by both strains occurred not only under anaerobic but also microaerobic conditions. It was suggested that Sb(V) reduction and the growth abilities of the strains under microaerobic conditions presented a substantial advantage of the use of strains AR-2 and AR-3 for practical applications to Sb(V)-containing wastewater treatment.https://www.mdpi.com/2076-2607/8/9/1435antimonate-reducing bacteriaantimony<i>Dechloromonas</i> sp.facultative anaerobe<i>Propionivibrio</i> sp. |
| spellingShingle | Ziran Yang Hisaaki Hosokawa Takuya Sadakane Masashi Kuroda Daisuke Inoue Hiroshi Nishikawa Michihiko Ike Isolation and Characterization of Facultative-Anaerobic Antimonate-Reducing Bacteria Microorganisms antimonate-reducing bacteria antimony <i>Dechloromonas</i> sp. facultative anaerobe <i>Propionivibrio</i> sp. |
| title | Isolation and Characterization of Facultative-Anaerobic Antimonate-Reducing Bacteria |
| title_full | Isolation and Characterization of Facultative-Anaerobic Antimonate-Reducing Bacteria |
| title_fullStr | Isolation and Characterization of Facultative-Anaerobic Antimonate-Reducing Bacteria |
| title_full_unstemmed | Isolation and Characterization of Facultative-Anaerobic Antimonate-Reducing Bacteria |
| title_short | Isolation and Characterization of Facultative-Anaerobic Antimonate-Reducing Bacteria |
| title_sort | isolation and characterization of facultative anaerobic antimonate reducing bacteria |
| topic | antimonate-reducing bacteria antimony <i>Dechloromonas</i> sp. facultative anaerobe <i>Propionivibrio</i> sp. |
| url | https://www.mdpi.com/2076-2607/8/9/1435 |
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