Key circular RNAs identified in male osteoporosis patients by whole transcriptome sequencing
Background Osteoporosis (OP) is a systemic disease with bone loss and microstructural deterioration. Numerous noncoding RNAs (ncRNAs) have been proved to participate in various diseases, especially circular RNAs (circRNAs). However, the expression profile and mechanisms underlying circRNAs in male o...
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PeerJ Inc.
2021-05-01
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| Online Access: | https://peerj.com/articles/11420.pdf |
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| author | Haijin Zhang Xue Song Zongyan Teng Sujun Cheng Weigang Yu Xiaoyi Yao Zhiqiang Song Yina Zhang |
| author_facet | Haijin Zhang Xue Song Zongyan Teng Sujun Cheng Weigang Yu Xiaoyi Yao Zhiqiang Song Yina Zhang |
| author_sort | Haijin Zhang |
| collection | DOAJ |
| description | Background Osteoporosis (OP) is a systemic disease with bone loss and microstructural deterioration. Numerous noncoding RNAs (ncRNAs) have been proved to participate in various diseases, especially circular RNAs (circRNAs). However, the expression profile and mechanisms underlying circRNAs in male osteoporosis have not yet been explored. Methods The whole transcriptome expression profile and differences in mRNAs, circRNAs, and microRNAs (miRNAs) were investigated in peripheral blood samples of patients with osteoporosis and healthy controls consisting of males ≥ 60-years-old. Results A total of 398 circRNAs, 51 miRNAs, and 642 mRNAs were significantly and differentially expressed in osteoporosis compared to healthy controls. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the host genes of significantly differentially expressed circRNAs were mainly enriched in the regulation of cell cycle process: biological process (BP), organelle part cellular components (CC), protein binding molecular function (MF), Toll-like receptor signaling pathway, tumor necrosis factor (TNF) signaling pathway, and thyroid hormone signaling pathway. circRNA-miRNA-mRNA regulatory network was constructed using the differentially expressed RNAs. Moreover, key circRNAs (hsa_circ_0042409) in osteoporosis were discovered and validated by qPCR. Conclusions The key cicrRNAs plays a major role in the pathogenesis of osteoporosis and could be used as potential biomarkers or targets in the diagnosis and treatment of osteoporosis. |
| first_indexed | 2024-03-09T07:53:38Z |
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| language | English |
| last_indexed | 2024-03-09T07:53:38Z |
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| spelling | doaj.art-750d7fea2330481290569cf1505c20662023-12-03T01:21:59ZengPeerJ Inc.PeerJ2167-83592021-05-019e1142010.7717/peerj.11420Key circular RNAs identified in male osteoporosis patients by whole transcriptome sequencingHaijin Zhang0Xue Song1Zongyan Teng2Sujun Cheng3Weigang Yu4Xiaoyi Yao5Zhiqiang Song6Yina Zhang7Department of Geriatrics, The Second Affiliated Hospital of Harbin Medical University, Harbin, ChinaHarbin North people’s Hospital, Harbin, ChinaDepartment of Geriatrics, The Second Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Geriatrics, The Second Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Geriatrics, The Second Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Geriatrics, The Second Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Geriatrics, The Second Affiliated Hospital of Harbin Medical University, Harbin, ChinaDepartment of Geriatrics, The Second Affiliated Hospital of Harbin Medical University, Harbin, ChinaBackground Osteoporosis (OP) is a systemic disease with bone loss and microstructural deterioration. Numerous noncoding RNAs (ncRNAs) have been proved to participate in various diseases, especially circular RNAs (circRNAs). However, the expression profile and mechanisms underlying circRNAs in male osteoporosis have not yet been explored. Methods The whole transcriptome expression profile and differences in mRNAs, circRNAs, and microRNAs (miRNAs) were investigated in peripheral blood samples of patients with osteoporosis and healthy controls consisting of males ≥ 60-years-old. Results A total of 398 circRNAs, 51 miRNAs, and 642 mRNAs were significantly and differentially expressed in osteoporosis compared to healthy controls. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis showed that the host genes of significantly differentially expressed circRNAs were mainly enriched in the regulation of cell cycle process: biological process (BP), organelle part cellular components (CC), protein binding molecular function (MF), Toll-like receptor signaling pathway, tumor necrosis factor (TNF) signaling pathway, and thyroid hormone signaling pathway. circRNA-miRNA-mRNA regulatory network was constructed using the differentially expressed RNAs. Moreover, key circRNAs (hsa_circ_0042409) in osteoporosis were discovered and validated by qPCR. Conclusions The key cicrRNAs plays a major role in the pathogenesis of osteoporosis and could be used as potential biomarkers or targets in the diagnosis and treatment of osteoporosis.https://peerj.com/articles/11420.pdfWhole transcriptome sequencingMale osteoporosisNon-coding RNAscircRNAs |
| spellingShingle | Haijin Zhang Xue Song Zongyan Teng Sujun Cheng Weigang Yu Xiaoyi Yao Zhiqiang Song Yina Zhang Key circular RNAs identified in male osteoporosis patients by whole transcriptome sequencing PeerJ Whole transcriptome sequencing Male osteoporosis Non-coding RNAs circRNAs |
| title | Key circular RNAs identified in male osteoporosis patients by whole transcriptome sequencing |
| title_full | Key circular RNAs identified in male osteoporosis patients by whole transcriptome sequencing |
| title_fullStr | Key circular RNAs identified in male osteoporosis patients by whole transcriptome sequencing |
| title_full_unstemmed | Key circular RNAs identified in male osteoporosis patients by whole transcriptome sequencing |
| title_short | Key circular RNAs identified in male osteoporosis patients by whole transcriptome sequencing |
| title_sort | key circular rnas identified in male osteoporosis patients by whole transcriptome sequencing |
| topic | Whole transcriptome sequencing Male osteoporosis Non-coding RNAs circRNAs |
| url | https://peerj.com/articles/11420.pdf |
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