Molecular Detection of <i>Toxoplasma gondii</i>, <i>Neospora caninum</i> and <i>Encephalitozoon</i> spp. in Vespertilionid Bats from Central Europe
Bats may carry various viruses and bacteria which can be harmful to humans, but little is known about their role as a parasitic source with zoonotic potential. The aim of this study was to test wild bats for the presence of selected parasites: <i>Toxoplasma gondii</i>, <i>Neospora...
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/12/9887 |
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| author | Eva Bártová Jiřina Marková Jana Sedláčková Hana Banďouchová Karol Račka |
| author_facet | Eva Bártová Jiřina Marková Jana Sedláčková Hana Banďouchová Karol Račka |
| author_sort | Eva Bártová |
| collection | DOAJ |
| description | Bats may carry various viruses and bacteria which can be harmful to humans, but little is known about their role as a parasitic source with zoonotic potential. The aim of this study was to test wild bats for the presence of selected parasites: <i>Toxoplasma gondii</i>, <i>Neospora caninum</i> and microsporidia <i>Encephalitozoon</i> spp. In total, brain and small intestine tissues of 100 bats (52 <i>Myotis myotis</i>, 43 <i>Nyctalus noctula</i> and 5 <i>Vespertilio murinus</i>) were used for the DNA isolation and PCR detection of the abovementioned agents. <i>Toxoplasma gondii</i> DNA was detected by real-time PCR in 1% of bats (in one male of <i>M. myotis</i>), while all bats were negative for <i>N. caninum</i> DNA. <i>Encephalitozoon</i> spp. DNA was detected by nested PCR in 25% of bats, including three species (twenty-two <i>M. myotis</i>, two <i>N. noctula</i> and one <i>V. murinus</i>). Positive samples were sequenced and showed homology with the genotypes <i>Encephalitozoon cuniculi</i> II and <i>Encephalitozoon hellem</i> 2C. This is the first study on wild vespertilionid bats from Central Europe and worldwide, with a relatively high positivity of <i>Encephalitozoon</i> spp. detected in bats. |
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| language | English |
| last_indexed | 2024-03-11T02:22:33Z |
| publishDate | 2023-06-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-750e69607f89439ebb8c65b4c925ebdd2023-11-18T10:45:30ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-012412988710.3390/ijms24129887Molecular Detection of <i>Toxoplasma gondii</i>, <i>Neospora caninum</i> and <i>Encephalitozoon</i> spp. in Vespertilionid Bats from Central EuropeEva Bártová0Jiřina Marková1Jana Sedláčková2Hana Banďouchová3Karol Račka4Department of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Palackého tř. 1946/1, 61242 Brno, Czech RepublicDepartment of Biology and Wildlife Diseases, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Palackého tř. 1946/1, 61242 Brno, Czech RepublicDepartment of Ecology and Diseases of Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Palackého tř. 1946/1, 61242 Brno, Czech RepublicDepartment of Ecology and Diseases of Game, Fish and Bees, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Palackého tř. 1946/1, 61242 Brno, Czech RepublicDepartment of Epizootology, Parasitology and Protection of One Health, University of Veterinary Medicine and Pharmacy in Košice, Komenského 73, 04181 Košice, SlovakiaBats may carry various viruses and bacteria which can be harmful to humans, but little is known about their role as a parasitic source with zoonotic potential. The aim of this study was to test wild bats for the presence of selected parasites: <i>Toxoplasma gondii</i>, <i>Neospora caninum</i> and microsporidia <i>Encephalitozoon</i> spp. In total, brain and small intestine tissues of 100 bats (52 <i>Myotis myotis</i>, 43 <i>Nyctalus noctula</i> and 5 <i>Vespertilio murinus</i>) were used for the DNA isolation and PCR detection of the abovementioned agents. <i>Toxoplasma gondii</i> DNA was detected by real-time PCR in 1% of bats (in one male of <i>M. myotis</i>), while all bats were negative for <i>N. caninum</i> DNA. <i>Encephalitozoon</i> spp. DNA was detected by nested PCR in 25% of bats, including three species (twenty-two <i>M. myotis</i>, two <i>N. noctula</i> and one <i>V. murinus</i>). Positive samples were sequenced and showed homology with the genotypes <i>Encephalitozoon cuniculi</i> II and <i>Encephalitozoon hellem</i> 2C. This is the first study on wild vespertilionid bats from Central Europe and worldwide, with a relatively high positivity of <i>Encephalitozoon</i> spp. detected in bats.https://www.mdpi.com/1422-0067/24/12/9887insectivoremicrosporidiosismolecular methodsneosporosistoxoplasmosis |
| spellingShingle | Eva Bártová Jiřina Marková Jana Sedláčková Hana Banďouchová Karol Račka Molecular Detection of <i>Toxoplasma gondii</i>, <i>Neospora caninum</i> and <i>Encephalitozoon</i> spp. in Vespertilionid Bats from Central Europe International Journal of Molecular Sciences insectivore microsporidiosis molecular methods neosporosis toxoplasmosis |
| title | Molecular Detection of <i>Toxoplasma gondii</i>, <i>Neospora caninum</i> and <i>Encephalitozoon</i> spp. in Vespertilionid Bats from Central Europe |
| title_full | Molecular Detection of <i>Toxoplasma gondii</i>, <i>Neospora caninum</i> and <i>Encephalitozoon</i> spp. in Vespertilionid Bats from Central Europe |
| title_fullStr | Molecular Detection of <i>Toxoplasma gondii</i>, <i>Neospora caninum</i> and <i>Encephalitozoon</i> spp. in Vespertilionid Bats from Central Europe |
| title_full_unstemmed | Molecular Detection of <i>Toxoplasma gondii</i>, <i>Neospora caninum</i> and <i>Encephalitozoon</i> spp. in Vespertilionid Bats from Central Europe |
| title_short | Molecular Detection of <i>Toxoplasma gondii</i>, <i>Neospora caninum</i> and <i>Encephalitozoon</i> spp. in Vespertilionid Bats from Central Europe |
| title_sort | molecular detection of i toxoplasma gondii i i neospora caninum i and i encephalitozoon i spp in vespertilionid bats from central europe |
| topic | insectivore microsporidiosis molecular methods neosporosis toxoplasmosis |
| url | https://www.mdpi.com/1422-0067/24/12/9887 |
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