EFFECT OF LOCAL RIFAMYCIN APPLICATION ON EXPRESSION OF BMP-2 AND BONE REGENERATION
Objectives: The aim of this study was to evaluate effect of local rifamycin application on BMP-2 expression and bone healing.Materials and Methods: A standardized 5.0-mm- diameter critical size bone defect was created mandible angulus region. In the control group (8 rats) defects were left empty. In...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Cumhuriyet University
2017-08-01
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| Series: | Cumhuriyet Dental Journal |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/download/article-file/353570 |
| Summary: | Objectives:
The aim of this study was to evaluate effect of local rifamycin application on
BMP-2 expression and bone healing.Materials and Methods:
A standardized 5.0-mm- diameter critical size bone defect was created mandible
angulus region. In the control group (8 rats) defects were left empty. In the
Group 1 (n=8 rats) defect was irrigated with rifamycin solution and 25 mg
rifamycin solution injected defect area at 1, 3, 7 days after surgery. In the
group 2 (n=8 rats) defects were grafted with a gelatin sponge mixed 25 mg
rifamycin solution. Rats were sacrificed at 21 days after surgery. Histological
slides were prepared from defect site for both immunohistochemical analysis
(bone morphogenetic protein-2 (BMP-2) antibody) and histomorphometric analysis.
Data were analyzed using Mann Whitney U and Kruskall Wallis test.Results:
The average new bone formation, number of osteoblast and new vessel formation
count were increased more in both of experimental groups in comparison with
control group. Anti-BMP-2 labelling (Cell count) was increased more in both of
experimental groups in comparison with control group.
Conclusion:
Local rifamycin application has positive effects on BMP-2 expression and bone regeneration
at critical sized bone defects. |
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| ISSN: | 1302-5805 2146-2852 |