Functionalization of Photosensitized Silica Nanoparticles for Advanced Photodynamic Therapy of Cancer
BODIPY dyes have recently attracted attention as potential photosensitizers. In this work, commercial and novel photosensitizers (PSs) based on BODIPY chromophores (haloBODIPYs and orthogonal dimers strategically designed with intense bands in the blue, green or red region of the visible spectra and...
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/12/6618 |
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| author | Ruth Prieto-Montero Alejandro Prieto-Castañeda Alberto Katsumiti Miren P. Cajaraville Antonia R. Agarrabeitia María J. Ortiz Virginia Martínez-Martínez |
| author_facet | Ruth Prieto-Montero Alejandro Prieto-Castañeda Alberto Katsumiti Miren P. Cajaraville Antonia R. Agarrabeitia María J. Ortiz Virginia Martínez-Martínez |
| author_sort | Ruth Prieto-Montero |
| collection | DOAJ |
| description | BODIPY dyes have recently attracted attention as potential photosensitizers. In this work, commercial and novel photosensitizers (PSs) based on BODIPY chromophores (haloBODIPYs and orthogonal dimers strategically designed with intense bands in the blue, green or red region of the visible spectra and high singlet oxygen production) were covalently linked to mesoporous silica nanoparticles (MSNs) further functionalized with PEG and folic acid (FA). MSNs approximately 50 nm in size with different functional groups were synthesized to allow multiple alternatives of PS-PEG-FA decoration of their external surface. Different combinations varying the type of PS (commercial Rose Bengal, Thionine and Chlorine e6 or custom-made BODIPY-based), the linkage design, and the length of PEG are detailed. All the nanosystems were physicochemically characterized (morphology, diameter, size distribution and PS loaded amount) and photophysically studied (absorption capacity, fluorescence efficiency, and singlet oxygen production) in suspension. For the most promising PS-PEG-FA silica nanoplatforms, the biocompatibility in dark conditions and the phototoxicity under suitable irradiation wavelengths (blue, green, or red) at regulated light doses (10–15 J/cm<sup>2</sup>) were compared with PSs free in solution in HeLa cells in vitro. |
| first_indexed | 2024-03-10T10:13:44Z |
| format | Article |
| id | doaj.art-75106e3ae80547cc84a423c8c79dd53f |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:13:44Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-75106e3ae80547cc84a423c8c79dd53f2023-11-22T01:00:30ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012212661810.3390/ijms22126618Functionalization of Photosensitized Silica Nanoparticles for Advanced Photodynamic Therapy of CancerRuth Prieto-Montero0Alejandro Prieto-Castañeda1Alberto Katsumiti2Miren P. Cajaraville3Antonia R. Agarrabeitia4María J. Ortiz5Virginia Martínez-Martínez6Departamento de Química Física, Universidad del País Vasco/Euskal Herriko Unibertsitatea (UPV/EHU), 48080 Bilbao, SpainDepartamento de Química Orgánica, Facultad de CC. Químicas, Universidad Complutense de Madrid, 28040 Madrid, SpainCBET Research Group, Department Zoology and Animal Cell Biology, Faculty of Science and Technology and Research Centre for Experimental Marine Biology and Biotechnology PiE, University of the Basque Country UPV/EHU, 48620 Basque Country, SpainCBET Research Group, Department Zoology and Animal Cell Biology, Faculty of Science and Technology and Research Centre for Experimental Marine Biology and Biotechnology PiE, University of the Basque Country UPV/EHU, 48620 Basque Country, SpainDepartamento de Química Orgánica, Facultad de CC. Químicas, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartamento de Química Orgánica, Facultad de CC. Químicas, Universidad Complutense de Madrid, 28040 Madrid, SpainDepartamento de Química Física, Universidad del País Vasco/Euskal Herriko Unibertsitatea (UPV/EHU), 48080 Bilbao, SpainBODIPY dyes have recently attracted attention as potential photosensitizers. In this work, commercial and novel photosensitizers (PSs) based on BODIPY chromophores (haloBODIPYs and orthogonal dimers strategically designed with intense bands in the blue, green or red region of the visible spectra and high singlet oxygen production) were covalently linked to mesoporous silica nanoparticles (MSNs) further functionalized with PEG and folic acid (FA). MSNs approximately 50 nm in size with different functional groups were synthesized to allow multiple alternatives of PS-PEG-FA decoration of their external surface. Different combinations varying the type of PS (commercial Rose Bengal, Thionine and Chlorine e6 or custom-made BODIPY-based), the linkage design, and the length of PEG are detailed. All the nanosystems were physicochemically characterized (morphology, diameter, size distribution and PS loaded amount) and photophysically studied (absorption capacity, fluorescence efficiency, and singlet oxygen production) in suspension. For the most promising PS-PEG-FA silica nanoplatforms, the biocompatibility in dark conditions and the phototoxicity under suitable irradiation wavelengths (blue, green, or red) at regulated light doses (10–15 J/cm<sup>2</sup>) were compared with PSs free in solution in HeLa cells in vitro.https://www.mdpi.com/1422-0067/22/12/6618BODIPY-based photosensitizersfunctionalized silica nanoparticlesfolic acidPEGphotodynamic therapyHeLa cells |
| spellingShingle | Ruth Prieto-Montero Alejandro Prieto-Castañeda Alberto Katsumiti Miren P. Cajaraville Antonia R. Agarrabeitia María J. Ortiz Virginia Martínez-Martínez Functionalization of Photosensitized Silica Nanoparticles for Advanced Photodynamic Therapy of Cancer International Journal of Molecular Sciences BODIPY-based photosensitizers functionalized silica nanoparticles folic acid PEG photodynamic therapy HeLa cells |
| title | Functionalization of Photosensitized Silica Nanoparticles for Advanced Photodynamic Therapy of Cancer |
| title_full | Functionalization of Photosensitized Silica Nanoparticles for Advanced Photodynamic Therapy of Cancer |
| title_fullStr | Functionalization of Photosensitized Silica Nanoparticles for Advanced Photodynamic Therapy of Cancer |
| title_full_unstemmed | Functionalization of Photosensitized Silica Nanoparticles for Advanced Photodynamic Therapy of Cancer |
| title_short | Functionalization of Photosensitized Silica Nanoparticles for Advanced Photodynamic Therapy of Cancer |
| title_sort | functionalization of photosensitized silica nanoparticles for advanced photodynamic therapy of cancer |
| topic | BODIPY-based photosensitizers functionalized silica nanoparticles folic acid PEG photodynamic therapy HeLa cells |
| url | https://www.mdpi.com/1422-0067/22/12/6618 |
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