Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft
Objective: To evaluate the possible association of a polymorphism in the gene encoding methylenetetrahydrofolate dehydrogenase 1 (MTHFD1), 1958G>A, with the susceptibility to orofacial cleft in an Indonesian population. Material and Methods: A total of 200 stored secondary biological samples from...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Association of Support to Oral Health Research (APESB)
2021-11-01
|
| Series: | Pesquisa Brasileira em Odontopediatria e Clínica Integrada |
| Subjects: | |
| Online Access: | https://revista.uepb.edu.br/PBOCI/article/view/668 |
| _version_ | 1818988528258777088 |
|---|---|
| author | Elza Ibrahim Auerkari Yolanda Bilynov Mindya Yuniastuti Listyowati Listyowati Lilies Dwi Sulistyani |
| author_facet | Elza Ibrahim Auerkari Yolanda Bilynov Mindya Yuniastuti Listyowati Listyowati Lilies Dwi Sulistyani |
| author_sort | Elza Ibrahim Auerkari |
| collection | DOAJ |
| description | Objective: To evaluate the possible association of a polymorphism in the gene encoding methylenetetrahydrofolate dehydrogenase 1 (MTHFD1), 1958G>A, with the susceptibility to orofacial cleft in an Indonesian population. Material and Methods: A total of 200 stored secondary biological samples from 30 cases of orofacial cleft and 170 unaffected controls were analyzed to determine the polymorphism status at base 1958. The analysis was conducted using the PCR-restriction fragment length polymorphism technique after digestion with the Msp1 restriction enzyme. The samples were then subjected to agarose gel electrophoresis to investigate the presence or absence of the following fragments: genotype GG, 196, 86 and 40 base pairs (bp); genotype AA, 282 and 28 bp and genotype AG, 282, 196, 86, 40 and 28 bp. The test groups were compared using the Chi-square test. Results: The wild-type allele containing 1958G, as well as the genotype GG, were significantly more common in the control group than in the orofacial cleft group. Conclusion: The MTHFD1 1958G>A polymorphism was significantly associated with orofacial cleft susceptibility in the tested Indonesian population. |
| first_indexed | 2024-12-20T19:24:01Z |
| format | Article |
| id | doaj.art-751715e160db4c5abe2c02b9c3a67fe1 |
| institution | Directory Open Access Journal |
| issn | 1519-0501 1983-4632 |
| language | English |
| last_indexed | 2024-12-20T19:24:01Z |
| publishDate | 2021-11-01 |
| publisher | Association of Support to Oral Health Research (APESB) |
| record_format | Article |
| series | Pesquisa Brasileira em Odontopediatria e Clínica Integrada |
| spelling | doaj.art-751715e160db4c5abe2c02b9c3a67fe12022-12-21T19:28:55ZengAssociation of Support to Oral Health Research (APESB)Pesquisa Brasileira em Odontopediatria e Clínica Integrada1519-05011983-46322021-11-0121Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial CleftElza Ibrahim AuerkariYolanda BilynovMindya YuniastutiListyowati ListyowatiLilies Dwi SulistyaniObjective: To evaluate the possible association of a polymorphism in the gene encoding methylenetetrahydrofolate dehydrogenase 1 (MTHFD1), 1958G>A, with the susceptibility to orofacial cleft in an Indonesian population. Material and Methods: A total of 200 stored secondary biological samples from 30 cases of orofacial cleft and 170 unaffected controls were analyzed to determine the polymorphism status at base 1958. The analysis was conducted using the PCR-restriction fragment length polymorphism technique after digestion with the Msp1 restriction enzyme. The samples were then subjected to agarose gel electrophoresis to investigate the presence or absence of the following fragments: genotype GG, 196, 86 and 40 base pairs (bp); genotype AA, 282 and 28 bp and genotype AG, 282, 196, 86, 40 and 28 bp. The test groups were compared using the Chi-square test. Results: The wild-type allele containing 1958G, as well as the genotype GG, were significantly more common in the control group than in the orofacial cleft group. Conclusion: The MTHFD1 1958G>A polymorphism was significantly associated with orofacial cleft susceptibility in the tested Indonesian population.https://revista.uepb.edu.br/PBOCI/article/view/668Cleft PalateCleft LipGenetic VariationPolymorphism, Genetic |
| spellingShingle | Elza Ibrahim Auerkari Yolanda Bilynov Mindya Yuniastuti Listyowati Listyowati Lilies Dwi Sulistyani Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft Pesquisa Brasileira em Odontopediatria e Clínica Integrada Cleft Palate Cleft Lip Genetic Variation Polymorphism, Genetic |
| title | Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft |
| title_full | Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft |
| title_fullStr | Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft |
| title_full_unstemmed | Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft |
| title_short | Association of a Polymorphism in the Gene Encoding Methylenetetrahydrofolate Dehydrogenase 1 (MTHFD1) 1958G>A with Orofacial Cleft |
| title_sort | association of a polymorphism in the gene encoding methylenetetrahydrofolate dehydrogenase 1 mthfd1 1958g a with orofacial cleft |
| topic | Cleft Palate Cleft Lip Genetic Variation Polymorphism, Genetic |
| url | https://revista.uepb.edu.br/PBOCI/article/view/668 |
| work_keys_str_mv | AT elzaibrahimauerkari associationofapolymorphisminthegeneencodingmethylenetetrahydrofolatedehydrogenase1mthfd11958gawithorofacialcleft AT yolandabilynov associationofapolymorphisminthegeneencodingmethylenetetrahydrofolatedehydrogenase1mthfd11958gawithorofacialcleft AT mindyayuniastuti associationofapolymorphisminthegeneencodingmethylenetetrahydrofolatedehydrogenase1mthfd11958gawithorofacialcleft AT listyowatilistyowati associationofapolymorphisminthegeneencodingmethylenetetrahydrofolatedehydrogenase1mthfd11958gawithorofacialcleft AT liliesdwisulistyani associationofapolymorphisminthegeneencodingmethylenetetrahydrofolatedehydrogenase1mthfd11958gawithorofacialcleft |