Application of Two Molecular Sexing Methods for Indonesian Bird Species: Implication for Captive Breeding Programs in Indonesia
Visually identifying the sex of a bird can be difficult. It cannot be done in half the world’s species when they are adults, and virtually none can be sexed as chicks. Despite this, the sex of a bird is vital for captive breeding. An increased number of birds are being sexed using DNA amplification...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2012-12-01
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| Series: | Hayati Journal of Biosciences |
| Subjects: | |
| Online Access: | http://journal.ipb.ac.id/index.php/hayati/article/view/6535/6535 |
| _version_ | 1811257995312496640 |
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| author | SRI SULANDARI MOCH SYAMSUL ARIFIN ZEIN |
| author_facet | SRI SULANDARI MOCH SYAMSUL ARIFIN ZEIN |
| author_sort | SRI SULANDARI |
| collection | DOAJ |
| description | Visually identifying the sex of a bird can be difficult. It cannot be done in half the world’s species when they are adults, and virtually none can be sexed as chicks. Despite this, the sex of a bird is vital for captive breeding. An increased number of birds are being sexed using DNA amplification techniques. In this approach, the CHD-W and CHD-Z are distinguished by the amplification of an intron present in both genes. PCR products on the gel electrophoresis vary in size revealing one band in males at the CHD-Z, and two bands in females corresponding to both the CHD-W and CHD-Z. Two independent sets of primer (P8/P2 and 2550F/2718R) were used to amplify the CHD gene region from both the Z and W chromosome. One hundred and ten (110) birds were sexed using first pair of primers: (P8/P2). Sexing results indicated that 81.8% were successfully determined, 12.7% failed to be amplified and 5.5% were not perfectly determined because the PCR products showed thick band. The thick band caused misidentified female to male birds. An alternative primer (2550F/2718R) was applied to solve the problem. Two hundreds and twenty-nine birds were sexed and the results showed 100% successfully determined. From this study, it is suggested to use a pair of 2550F and 2718R primers for distinguishing a male from a female bird. |
| first_indexed | 2024-04-12T18:06:18Z |
| format | Article |
| id | doaj.art-751b1e3c00ae4a4f8a10a544be0c5043 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-04-12T18:06:18Z |
| publishDate | 2012-12-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-751b1e3c00ae4a4f8a10a544be0c50432022-12-22T03:21:58ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942012-12-01194183190Application of Two Molecular Sexing Methods for Indonesian Bird Species: Implication for Captive Breeding Programs in IndonesiaSRI SULANDARIMOCH SYAMSUL ARIFIN ZEINVisually identifying the sex of a bird can be difficult. It cannot be done in half the world’s species when they are adults, and virtually none can be sexed as chicks. Despite this, the sex of a bird is vital for captive breeding. An increased number of birds are being sexed using DNA amplification techniques. In this approach, the CHD-W and CHD-Z are distinguished by the amplification of an intron present in both genes. PCR products on the gel electrophoresis vary in size revealing one band in males at the CHD-Z, and two bands in females corresponding to both the CHD-W and CHD-Z. Two independent sets of primer (P8/P2 and 2550F/2718R) were used to amplify the CHD gene region from both the Z and W chromosome. One hundred and ten (110) birds were sexed using first pair of primers: (P8/P2). Sexing results indicated that 81.8% were successfully determined, 12.7% failed to be amplified and 5.5% were not perfectly determined because the PCR products showed thick band. The thick band caused misidentified female to male birds. An alternative primer (2550F/2718R) was applied to solve the problem. Two hundreds and twenty-nine birds were sexed and the results showed 100% successfully determined. From this study, it is suggested to use a pair of 2550F and 2718R primers for distinguishing a male from a female bird.http://journal.ipb.ac.id/index.php/hayati/article/view/6535/6535sex identificationIndonesian birdsprimer sexingPCRcaptive breeding |
| spellingShingle | SRI SULANDARI MOCH SYAMSUL ARIFIN ZEIN Application of Two Molecular Sexing Methods for Indonesian Bird Species: Implication for Captive Breeding Programs in Indonesia Hayati Journal of Biosciences sex identification Indonesian birds primer sexing PCR captive breeding |
| title | Application of Two Molecular Sexing Methods for Indonesian Bird Species: Implication for Captive Breeding Programs in Indonesia |
| title_full | Application of Two Molecular Sexing Methods for Indonesian Bird Species: Implication for Captive Breeding Programs in Indonesia |
| title_fullStr | Application of Two Molecular Sexing Methods for Indonesian Bird Species: Implication for Captive Breeding Programs in Indonesia |
| title_full_unstemmed | Application of Two Molecular Sexing Methods for Indonesian Bird Species: Implication for Captive Breeding Programs in Indonesia |
| title_short | Application of Two Molecular Sexing Methods for Indonesian Bird Species: Implication for Captive Breeding Programs in Indonesia |
| title_sort | application of two molecular sexing methods for indonesian bird species implication for captive breeding programs in indonesia |
| topic | sex identification Indonesian birds primer sexing PCR captive breeding |
| url | http://journal.ipb.ac.id/index.php/hayati/article/view/6535/6535 |
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