The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B
Staphylococcal enterotoxin B (SEB) is a protein produced by <i>Staphylococcus aureus</i>, which is toxic to humans. It is well known for its ability to stimulate the exacerbated activation of proinflammatory CD4+ T cells (Th1 profile), and in vitro studies have been conducted to understa...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/8/3480 |
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| author | Leslie Chavez-Galan Andy Ruiz Lucero A. Ramón-Luing Alejandro Escamilla-Gutiérrez Anahí Sánchez-Monciváis Brenda Tecuatzi-Cadena Karen Medina-Quero María Guadalupe Córdova-Espinoza |
| author_facet | Leslie Chavez-Galan Andy Ruiz Lucero A. Ramón-Luing Alejandro Escamilla-Gutiérrez Anahí Sánchez-Monciváis Brenda Tecuatzi-Cadena Karen Medina-Quero María Guadalupe Córdova-Espinoza |
| author_sort | Leslie Chavez-Galan |
| collection | DOAJ |
| description | Staphylococcal enterotoxin B (SEB) is a protein produced by <i>Staphylococcus aureus</i>, which is toxic to humans. It is well known for its ability to stimulate the exacerbated activation of proinflammatory CD4+ T cells (Th1 profile), and in vitro studies have been conducted to understand its mechanism of action and its potential use as an immune therapy. However, the efficiency of the SEB1741 aptamer in blocking SEB has not been experimentally demonstrated. Methods: Enrichment CD4+ T cells were stimulated with SEB, and as a blocker, we used the SEB1741 aptamer, which was previously synthesised by an “in silico” analysis, showing high affinity and specificity to SEB. The efficiency of the SEB1741 aptamer in blocking CD4+ T cell activation was compared with that of an anti-SEB monoclonal antibody. Flow cytometry and Bio-Plex were used to evaluate the T-cell function. Results: In vitro, SEB induced the activation of CD4+ T cells and favoured a Th1 profile; however, the SEB1741 aptamer was highly efficient in decreasing the frequency of CD4+ T cells positive to ki-67 and CD69 cells, this means that proliferation and activation of CD4+ T cells was decreased. Moreover, the production of interleukin 2 (IL-2) and interferon-gamma (IFN-γ) was affected, suggesting that the Th1 profile is not present when the SEB1441 aptamer is used. Thus, the SEB1741 function was similar to that of anti-SEB. Conclusions: The SEB1741 aptamer is a valuable tool for blocking CD4+ T cell activation and the subsequent release of proinflammatory cytokines by SEB stimulation. |
| first_indexed | 2024-03-11T04:41:33Z |
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| id | doaj.art-751ef28310f0487fad5cdc8862ea7d97 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T04:41:33Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-751ef28310f0487fad5cdc8862ea7d972023-11-17T20:39:33ZengMDPI AGMolecules1420-30492023-04-01288348010.3390/molecules28083480The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin BLeslie Chavez-Galan0Andy Ruiz1Lucero A. Ramón-Luing2Alejandro Escamilla-Gutiérrez3Anahí Sánchez-Monciváis4Brenda Tecuatzi-Cadena5Karen Medina-Quero6María Guadalupe Córdova-Espinoza7Laboratory of Integrative Immunology, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoLaboratory of Integrative Immunology, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoLaboratory of Integrative Immunology, Instituto Nacional de Enfermedades Respiratorias Ismael Cosío Villegas, Mexico City 14080, MexicoLaboratory of Medical Bacteriology, Department of Microbiology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11350, MexicoLaboratory of Immunology, Escuela Militar de Graduados de Sanidad, SEDENA, Mexico City 11200, MexicoLaboratory of Immunology, Escuela Militar de Graduados de Sanidad, SEDENA, Mexico City 11200, MexicoLaboratory of Immunology, Escuela Militar de Graduados de Sanidad, SEDENA, Mexico City 11200, MexicoLaboratory of Medical Bacteriology, Department of Microbiology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City 11350, MexicoStaphylococcal enterotoxin B (SEB) is a protein produced by <i>Staphylococcus aureus</i>, which is toxic to humans. It is well known for its ability to stimulate the exacerbated activation of proinflammatory CD4+ T cells (Th1 profile), and in vitro studies have been conducted to understand its mechanism of action and its potential use as an immune therapy. However, the efficiency of the SEB1741 aptamer in blocking SEB has not been experimentally demonstrated. Methods: Enrichment CD4+ T cells were stimulated with SEB, and as a blocker, we used the SEB1741 aptamer, which was previously synthesised by an “in silico” analysis, showing high affinity and specificity to SEB. The efficiency of the SEB1741 aptamer in blocking CD4+ T cell activation was compared with that of an anti-SEB monoclonal antibody. Flow cytometry and Bio-Plex were used to evaluate the T-cell function. Results: In vitro, SEB induced the activation of CD4+ T cells and favoured a Th1 profile; however, the SEB1741 aptamer was highly efficient in decreasing the frequency of CD4+ T cells positive to ki-67 and CD69 cells, this means that proliferation and activation of CD4+ T cells was decreased. Moreover, the production of interleukin 2 (IL-2) and interferon-gamma (IFN-γ) was affected, suggesting that the Th1 profile is not present when the SEB1441 aptamer is used. Thus, the SEB1741 function was similar to that of anti-SEB. Conclusions: The SEB1741 aptamer is a valuable tool for blocking CD4+ T cell activation and the subsequent release of proinflammatory cytokines by SEB stimulation.https://www.mdpi.com/1420-3049/28/8/3480SEBanti-SEBSEB1741 aptamerCD4+ T cellsinflammation |
| spellingShingle | Leslie Chavez-Galan Andy Ruiz Lucero A. Ramón-Luing Alejandro Escamilla-Gutiérrez Anahí Sánchez-Monciváis Brenda Tecuatzi-Cadena Karen Medina-Quero María Guadalupe Córdova-Espinoza The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B Molecules SEB anti-SEB SEB1741 aptamer CD4+ T cells inflammation |
| title | The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B |
| title_full | The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B |
| title_fullStr | The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B |
| title_full_unstemmed | The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B |
| title_short | The SEB1741 Aptamer Is an Efficient Tool for Blocking CD4+ T Cell Activation Induced by Staphylococcal Enterotoxin B |
| title_sort | seb1741 aptamer is an efficient tool for blocking cd4 t cell activation induced by staphylococcal enterotoxin b |
| topic | SEB anti-SEB SEB1741 aptamer CD4+ T cells inflammation |
| url | https://www.mdpi.com/1420-3049/28/8/3480 |
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