Altered intragenic DNA methylation of HOOK2 gene in adipose tissue from individuals with obesity and type 2 diabetes.
Failure in glucose response to insulin is a common pathology associated with obesity. In this study, we analyzed the genome wide DNA methylation profile of visceral adipose tissue (VAT) samples in a population of individuals with obesity and assessed whether differential methylation profiles are ass...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2017-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5724849?pdf=render |
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| author | Sandra Rodríguez-Rodero Edelmiro Menéndez-Torre Gustavo Fernández-Bayón Paula Morales-Sánchez Lourdes Sanz Estrella Turienzo Juan José González Ceferino Martinez-Faedo Lorena Suarez-Gutiérrez Jessica Ares Lucia Díaz-Naya Alicia Martin-Nieto Juan L Fernández-Morera Mario F Fraga Elías Delgado-Álvarez |
| author_facet | Sandra Rodríguez-Rodero Edelmiro Menéndez-Torre Gustavo Fernández-Bayón Paula Morales-Sánchez Lourdes Sanz Estrella Turienzo Juan José González Ceferino Martinez-Faedo Lorena Suarez-Gutiérrez Jessica Ares Lucia Díaz-Naya Alicia Martin-Nieto Juan L Fernández-Morera Mario F Fraga Elías Delgado-Álvarez |
| author_sort | Sandra Rodríguez-Rodero |
| collection | DOAJ |
| description | Failure in glucose response to insulin is a common pathology associated with obesity. In this study, we analyzed the genome wide DNA methylation profile of visceral adipose tissue (VAT) samples in a population of individuals with obesity and assessed whether differential methylation profiles are associated with the presence of type 2 diabetes (T2D).More than 485,000 CpG genome sites from VAT samples from women with obesity undergoing gastric bypass (n = 18), and classified as suffering from type 2 diabetes (T2D) or not (no type 2 diabetes, NT2D), were analyzed using DNA methylation arrays.We found significant differential methylation between T2D and NT2D samples in 24 CpGs that map with sixteen genes, one of which, HOOK2, demonstrated a significant correlation between differentially hypermethylated regions on the gene body and the presence of type 2 diabetes. This was validated by pyrosequencing in a population of 91 samples from both males and females with obesity. Furthermore, when these results were analyzed by gender, female T2D samples were found hypermethylated at the cg04657146-region and the cg 11738485-region of HOOK2 gene, whilst, interestingly, male samples were found hypomethylated in this latter region.The differential methylation profile of the HOOK2 gene in individuals with T2D and obesity might be related to the attendant T2D, but further studies are required to identify the potential role of HOOK2 gene in T2D disease. The finding of gender differences in T2D methylation of HOOK2 also warrants further investigation. |
| first_indexed | 2024-04-12T19:11:15Z |
| format | Article |
| id | doaj.art-7526b02fb2534c30bb9d622711e058c0 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T19:11:15Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7526b02fb2534c30bb9d622711e058c02022-12-22T03:19:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-011212e018915310.1371/journal.pone.0189153Altered intragenic DNA methylation of HOOK2 gene in adipose tissue from individuals with obesity and type 2 diabetes.Sandra Rodríguez-RoderoEdelmiro Menéndez-TorreGustavo Fernández-BayónPaula Morales-SánchezLourdes SanzEstrella TurienzoJuan José GonzálezCeferino Martinez-FaedoLorena Suarez-GutiérrezJessica AresLucia Díaz-NayaAlicia Martin-NietoJuan L Fernández-MoreraMario F FragaElías Delgado-ÁlvarezFailure in glucose response to insulin is a common pathology associated with obesity. In this study, we analyzed the genome wide DNA methylation profile of visceral adipose tissue (VAT) samples in a population of individuals with obesity and assessed whether differential methylation profiles are associated with the presence of type 2 diabetes (T2D).More than 485,000 CpG genome sites from VAT samples from women with obesity undergoing gastric bypass (n = 18), and classified as suffering from type 2 diabetes (T2D) or not (no type 2 diabetes, NT2D), were analyzed using DNA methylation arrays.We found significant differential methylation between T2D and NT2D samples in 24 CpGs that map with sixteen genes, one of which, HOOK2, demonstrated a significant correlation between differentially hypermethylated regions on the gene body and the presence of type 2 diabetes. This was validated by pyrosequencing in a population of 91 samples from both males and females with obesity. Furthermore, when these results were analyzed by gender, female T2D samples were found hypermethylated at the cg04657146-region and the cg 11738485-region of HOOK2 gene, whilst, interestingly, male samples were found hypomethylated in this latter region.The differential methylation profile of the HOOK2 gene in individuals with T2D and obesity might be related to the attendant T2D, but further studies are required to identify the potential role of HOOK2 gene in T2D disease. The finding of gender differences in T2D methylation of HOOK2 also warrants further investigation.http://europepmc.org/articles/PMC5724849?pdf=render |
| spellingShingle | Sandra Rodríguez-Rodero Edelmiro Menéndez-Torre Gustavo Fernández-Bayón Paula Morales-Sánchez Lourdes Sanz Estrella Turienzo Juan José González Ceferino Martinez-Faedo Lorena Suarez-Gutiérrez Jessica Ares Lucia Díaz-Naya Alicia Martin-Nieto Juan L Fernández-Morera Mario F Fraga Elías Delgado-Álvarez Altered intragenic DNA methylation of HOOK2 gene in adipose tissue from individuals with obesity and type 2 diabetes. PLoS ONE |
| title | Altered intragenic DNA methylation of HOOK2 gene in adipose tissue from individuals with obesity and type 2 diabetes. |
| title_full | Altered intragenic DNA methylation of HOOK2 gene in adipose tissue from individuals with obesity and type 2 diabetes. |
| title_fullStr | Altered intragenic DNA methylation of HOOK2 gene in adipose tissue from individuals with obesity and type 2 diabetes. |
| title_full_unstemmed | Altered intragenic DNA methylation of HOOK2 gene in adipose tissue from individuals with obesity and type 2 diabetes. |
| title_short | Altered intragenic DNA methylation of HOOK2 gene in adipose tissue from individuals with obesity and type 2 diabetes. |
| title_sort | altered intragenic dna methylation of hook2 gene in adipose tissue from individuals with obesity and type 2 diabetes |
| url | http://europepmc.org/articles/PMC5724849?pdf=render |
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