Influence of Perinatal Factors on Gene Expression of IAPs Family and Main Factors of Pluripotency: <i>OCT4</i> and <i>SOX2</i> in Human Breast Milk Stem Cells—A Preliminary Report
Due to their therapeutic potential, mesenchymal stem cells are the subject of intensive research on the use of their potential in the treatment of, among others, neurodegenerative diseases or immunological diseases. They are among the newest in the field of medicine. The presented study aimed to eva...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/3/2476 |
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| author | Paulina Gil-Kulik Michał Leśniewski Karolina Bieńko Monika Wójcik Marta Więckowska Dominika Przywara Alicja Petniak Adrianna Kondracka Małgorzata Świstowska Rafał Szymanowski Agnieszka Wilińska Mateusz Wiliński Bartosz J. Płachno Marzena Kostuch Mansur Rahnama-Hezavach Mariusz Szuta Anna Kwaśniewska Anna Bogucka-Kocka Janusz Kocki |
| author_facet | Paulina Gil-Kulik Michał Leśniewski Karolina Bieńko Monika Wójcik Marta Więckowska Dominika Przywara Alicja Petniak Adrianna Kondracka Małgorzata Świstowska Rafał Szymanowski Agnieszka Wilińska Mateusz Wiliński Bartosz J. Płachno Marzena Kostuch Mansur Rahnama-Hezavach Mariusz Szuta Anna Kwaśniewska Anna Bogucka-Kocka Janusz Kocki |
| author_sort | Paulina Gil-Kulik |
| collection | DOAJ |
| description | Due to their therapeutic potential, mesenchymal stem cells are the subject of intensive research on the use of their potential in the treatment of, among others, neurodegenerative diseases or immunological diseases. They are among the newest in the field of medicine. The presented study aimed to evaluate the expression of eight genes from the IAP family and the gene regulating IAP—<i>XAF1</i>—in stem cells derived from human milk, using the qPCR method. The relationships between the expression of genes under study and clinical data, such as maternal age, maternal BMI, week of pregnancy in which the delivery took place, bodyweight of the newborn, the number of pregnancies and deliveries, and the time elapsed since delivery, were also analyzed. The research was carried out on samples of human milk collected from 42 patients hospitalized in The Clinic of Obstetrics and Perinatology of the Independent Public Clinical Hospital No. 4, in Lublin. The conducted research confirmed the expression of the following genes in the tested material: <i>NAIP, BIRC2, BIRC3, BIRC5, BIRC6, BIRC8, XIAP, XAF1, OCT4</i> and <i>SOX2</i>. Moreover, several dependencies of the expression of individual genes on the maternal BMI (<i>BIRC5, XAF1</i> and <i>NAIP</i>), the time since childbirth (<i>BIRC5, BIRC6, XAF1</i> and <i>NAIP</i>), the number of pregnancies and deliveries (<i>BIRC2, BIRC5, BIRC6</i> and <i>XAF1</i>), the manner of delivery (<i>XAF1</i> and <i>OCT4</i>), preterm labor (<i>BIRC6</i> and <i>NAIP</i>) were demonstrated. Additionally, we found positive relationships between gene expression of BIRC7, BIRC8 and XAF1 and the main factors of pluripotency: <i>SOX2</i> and <i>OCT4</i>. This work is the first to investigate the expression of genes from the IAPs family in mother’s milk stem cells. |
| first_indexed | 2024-03-11T09:40:54Z |
| format | Article |
| id | doaj.art-7527083b3bc14af088fe27f56e8747b3 |
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| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T09:40:54Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-7527083b3bc14af088fe27f56e8747b32023-11-16T16:57:52ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-01-01243247610.3390/ijms24032476Influence of Perinatal Factors on Gene Expression of IAPs Family and Main Factors of Pluripotency: <i>OCT4</i> and <i>SOX2</i> in Human Breast Milk Stem Cells—A Preliminary ReportPaulina Gil-Kulik0Michał Leśniewski1Karolina Bieńko2Monika Wójcik3Marta Więckowska4Dominika Przywara5Alicja Petniak6Adrianna Kondracka7Małgorzata Świstowska8Rafał Szymanowski9Agnieszka Wilińska10Mateusz Wiliński11Bartosz J. Płachno12Marzena Kostuch13Mansur Rahnama-Hezavach14Mariusz Szuta15Anna Kwaśniewska16Anna Bogucka-Kocka17Janusz Kocki18Department of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska Str., 20-080 Lublin, PolandStudent Scientific Society of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska Str., 20-080 Lublin, PolandStudent Scientific Society of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska Str., 20-080 Lublin, PolandStudent Scientific Society of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska Str., 20-080 Lublin, PolandStudent Scientific Society of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska Str., 20-080 Lublin, PolandDepartment of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska Str., 20-080 Lublin, PolandDepartment of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska Str., 20-080 Lublin, PolandDepartment of Obstetrics and Pathology of Pregnancy, Medical University of Lublin, 11 Staszica Str., 20-081 Lublin, PolandDepartment of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska Str., 20-080 Lublin, PolandDepartment of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska Str., 20-080 Lublin, PolandDepartment of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska Str., 20-080 Lublin, PolandDepartment of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska Str., 20-080 Lublin, PolandDepartment of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University in Kraków, 9 Gronostajowa St., 30-387 Cracow, PolandDepartment of Neonatology, Independent Public Clinical Hospital No. 4, 8 Jaczewskiego St., 20-954 Lublin, PolandChair and Department of Dental Surgery, Medical University of Lublin, 6 Chodzki St., 20-093 Lublin, PolandChair of Oral Surgery, Jagiellonian University Medical College, 4 Montelupich St., 31-155 Kraków, PolandDepartment of Obstetrics and Pathology of Pregnancy, Medical University of Lublin, 11 Staszica Str., 20-081 Lublin, PolandChair and Department of Biology and Genetics, Medical University of Lublin, 4a Chodźki St., 20–093 Lublin, PolandDepartment of Clinical Genetics, Medical University of Lublin, 11 Radziwillowska Str., 20-080 Lublin, PolandDue to their therapeutic potential, mesenchymal stem cells are the subject of intensive research on the use of their potential in the treatment of, among others, neurodegenerative diseases or immunological diseases. They are among the newest in the field of medicine. The presented study aimed to evaluate the expression of eight genes from the IAP family and the gene regulating IAP—<i>XAF1</i>—in stem cells derived from human milk, using the qPCR method. The relationships between the expression of genes under study and clinical data, such as maternal age, maternal BMI, week of pregnancy in which the delivery took place, bodyweight of the newborn, the number of pregnancies and deliveries, and the time elapsed since delivery, were also analyzed. The research was carried out on samples of human milk collected from 42 patients hospitalized in The Clinic of Obstetrics and Perinatology of the Independent Public Clinical Hospital No. 4, in Lublin. The conducted research confirmed the expression of the following genes in the tested material: <i>NAIP, BIRC2, BIRC3, BIRC5, BIRC6, BIRC8, XIAP, XAF1, OCT4</i> and <i>SOX2</i>. Moreover, several dependencies of the expression of individual genes on the maternal BMI (<i>BIRC5, XAF1</i> and <i>NAIP</i>), the time since childbirth (<i>BIRC5, BIRC6, XAF1</i> and <i>NAIP</i>), the number of pregnancies and deliveries (<i>BIRC2, BIRC5, BIRC6</i> and <i>XAF1</i>), the manner of delivery (<i>XAF1</i> and <i>OCT4</i>), preterm labor (<i>BIRC6</i> and <i>NAIP</i>) were demonstrated. Additionally, we found positive relationships between gene expression of BIRC7, BIRC8 and XAF1 and the main factors of pluripotency: <i>SOX2</i> and <i>OCT4</i>. This work is the first to investigate the expression of genes from the IAPs family in mother’s milk stem cells.https://www.mdpi.com/1422-0067/24/3/2476breast milk stem cellsIAPsBIRCOCT4SOX2gene expression |
| spellingShingle | Paulina Gil-Kulik Michał Leśniewski Karolina Bieńko Monika Wójcik Marta Więckowska Dominika Przywara Alicja Petniak Adrianna Kondracka Małgorzata Świstowska Rafał Szymanowski Agnieszka Wilińska Mateusz Wiliński Bartosz J. Płachno Marzena Kostuch Mansur Rahnama-Hezavach Mariusz Szuta Anna Kwaśniewska Anna Bogucka-Kocka Janusz Kocki Influence of Perinatal Factors on Gene Expression of IAPs Family and Main Factors of Pluripotency: <i>OCT4</i> and <i>SOX2</i> in Human Breast Milk Stem Cells—A Preliminary Report International Journal of Molecular Sciences breast milk stem cells IAPs BIRC OCT4 SOX2 gene expression |
| title | Influence of Perinatal Factors on Gene Expression of IAPs Family and Main Factors of Pluripotency: <i>OCT4</i> and <i>SOX2</i> in Human Breast Milk Stem Cells—A Preliminary Report |
| title_full | Influence of Perinatal Factors on Gene Expression of IAPs Family and Main Factors of Pluripotency: <i>OCT4</i> and <i>SOX2</i> in Human Breast Milk Stem Cells—A Preliminary Report |
| title_fullStr | Influence of Perinatal Factors on Gene Expression of IAPs Family and Main Factors of Pluripotency: <i>OCT4</i> and <i>SOX2</i> in Human Breast Milk Stem Cells—A Preliminary Report |
| title_full_unstemmed | Influence of Perinatal Factors on Gene Expression of IAPs Family and Main Factors of Pluripotency: <i>OCT4</i> and <i>SOX2</i> in Human Breast Milk Stem Cells—A Preliminary Report |
| title_short | Influence of Perinatal Factors on Gene Expression of IAPs Family and Main Factors of Pluripotency: <i>OCT4</i> and <i>SOX2</i> in Human Breast Milk Stem Cells—A Preliminary Report |
| title_sort | influence of perinatal factors on gene expression of iaps family and main factors of pluripotency i oct4 i and i sox2 i in human breast milk stem cells a preliminary report |
| topic | breast milk stem cells IAPs BIRC OCT4 SOX2 gene expression |
| url | https://www.mdpi.com/1422-0067/24/3/2476 |
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