Lycosin-II Exhibits Antifungal Activity and Inhibits Dual-Species Biofilm by <i>Candida albicans</i> and <i>Staphylococcus aureus</i>
The increase and dissemination of antimicrobial resistance is a global public health issue. To address this, new antimicrobial agents have been developed. Antimicrobial peptides (AMPs) exhibit a wide range of antimicrobial activities against pathogens, including bacteria and fungi. Lycosin-II, isola...
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MDPI AG
2022-08-01
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| Series: | Journal of Fungi |
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| Online Access: | https://www.mdpi.com/2309-608X/8/9/901 |
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| author | Jonggwan Park Hyeongsun Kim Hee-Kyoung Kang Moon-Chang Choi Yoonkyung Park |
| author_facet | Jonggwan Park Hyeongsun Kim Hee-Kyoung Kang Moon-Chang Choi Yoonkyung Park |
| author_sort | Jonggwan Park |
| collection | DOAJ |
| description | The increase and dissemination of antimicrobial resistance is a global public health issue. To address this, new antimicrobial agents have been developed. Antimicrobial peptides (AMPs) exhibit a wide range of antimicrobial activities against pathogens, including bacteria and fungi. Lycosin-II, isolated from the venom of the spider <i>Lycosa singoriensis</i>, has shown antibacterial activity by disrupting membranes. However, the mode of action of Lycosin-II and its antifungal activity have not been clearly described. Therefore, we confirmed that Lycosin-II showed antifungal activity against <i>Candida albicans</i> (<i>C. albicans</i>). To investigate the mode of action, membrane-related assays were performed, including an evaluation of <i>C. albicans</i> membrane depolarization and membrane integrity after exposure to Lycosin-II. Our results indicated that Lycosin-II damaged the <i>C. albicans</i> membrane. Additionally, Lycosin-II induced oxidative stress through the generation of reactive oxygen species (ROS) in <i>C. albicans</i>. Moreover, Lycosin-II exhibited an inhibitory effect on dual-species biofilm formation by <i>C. albicans</i> and <i>Staphylococcus aureus</i> (<i>S. aureus</i>)<i>,</i> which are the most co-isolated fungi and bacteria. These results revealed that Lycosin-II can be utilized against <i>C. albicans</i> and dual-species strain infections. |
| first_indexed | 2024-03-09T23:31:14Z |
| format | Article |
| id | doaj.art-75275bb008f7491c9a24605aa7e68e48 |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-09T23:31:14Z |
| publishDate | 2022-08-01 |
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| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-75275bb008f7491c9a24605aa7e68e482023-11-23T17:08:57ZengMDPI AGJournal of Fungi2309-608X2022-08-018990110.3390/jof8090901Lycosin-II Exhibits Antifungal Activity and Inhibits Dual-Species Biofilm by <i>Candida albicans</i> and <i>Staphylococcus aureus</i>Jonggwan Park0Hyeongsun Kim1Hee-Kyoung Kang2Moon-Chang Choi3Yoonkyung Park4Department of Bioinformatics, Kongju National University, Kongju 32588, KoreaDepartment of Biomedical Science, Chosun University, Gwangju 61452, KoreaDepartment of Biomedical Science, Chosun University, Gwangju 61452, KoreaDepartment of Biomedical Science, Chosun University, Gwangju 61452, KoreaDepartment of Biomedical Science, Chosun University, Gwangju 61452, KoreaThe increase and dissemination of antimicrobial resistance is a global public health issue. To address this, new antimicrobial agents have been developed. Antimicrobial peptides (AMPs) exhibit a wide range of antimicrobial activities against pathogens, including bacteria and fungi. Lycosin-II, isolated from the venom of the spider <i>Lycosa singoriensis</i>, has shown antibacterial activity by disrupting membranes. However, the mode of action of Lycosin-II and its antifungal activity have not been clearly described. Therefore, we confirmed that Lycosin-II showed antifungal activity against <i>Candida albicans</i> (<i>C. albicans</i>). To investigate the mode of action, membrane-related assays were performed, including an evaluation of <i>C. albicans</i> membrane depolarization and membrane integrity after exposure to Lycosin-II. Our results indicated that Lycosin-II damaged the <i>C. albicans</i> membrane. Additionally, Lycosin-II induced oxidative stress through the generation of reactive oxygen species (ROS) in <i>C. albicans</i>. Moreover, Lycosin-II exhibited an inhibitory effect on dual-species biofilm formation by <i>C. albicans</i> and <i>Staphylococcus aureus</i> (<i>S. aureus</i>)<i>,</i> which are the most co-isolated fungi and bacteria. These results revealed that Lycosin-II can be utilized against <i>C. albicans</i> and dual-species strain infections.https://www.mdpi.com/2309-608X/8/9/901antimicrobial peptide<i>Candida albicans</i><i>Staphylococcus aureus</i> |
| spellingShingle | Jonggwan Park Hyeongsun Kim Hee-Kyoung Kang Moon-Chang Choi Yoonkyung Park Lycosin-II Exhibits Antifungal Activity and Inhibits Dual-Species Biofilm by <i>Candida albicans</i> and <i>Staphylococcus aureus</i> Journal of Fungi antimicrobial peptide <i>Candida albicans</i> <i>Staphylococcus aureus</i> |
| title | Lycosin-II Exhibits Antifungal Activity and Inhibits Dual-Species Biofilm by <i>Candida albicans</i> and <i>Staphylococcus aureus</i> |
| title_full | Lycosin-II Exhibits Antifungal Activity and Inhibits Dual-Species Biofilm by <i>Candida albicans</i> and <i>Staphylococcus aureus</i> |
| title_fullStr | Lycosin-II Exhibits Antifungal Activity and Inhibits Dual-Species Biofilm by <i>Candida albicans</i> and <i>Staphylococcus aureus</i> |
| title_full_unstemmed | Lycosin-II Exhibits Antifungal Activity and Inhibits Dual-Species Biofilm by <i>Candida albicans</i> and <i>Staphylococcus aureus</i> |
| title_short | Lycosin-II Exhibits Antifungal Activity and Inhibits Dual-Species Biofilm by <i>Candida albicans</i> and <i>Staphylococcus aureus</i> |
| title_sort | lycosin ii exhibits antifungal activity and inhibits dual species biofilm by i candida albicans i and i staphylococcus aureus i |
| topic | antimicrobial peptide <i>Candida albicans</i> <i>Staphylococcus aureus</i> |
| url | https://www.mdpi.com/2309-608X/8/9/901 |
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