Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1).
Lactate has long been regarded as an end product of anaerobic energy production and its fate in cerebral metabolism has not been precisely delineated. In this report, we demonstrate, for the first time, the ability of a human astrocytic cell line (CCF-STTG1) to consume lactate and to generate ATP vi...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2008-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC2212712?pdf=render |
| _version_ | 1818202083715710976 |
|---|---|
| author | Joseph Lemire Ryan J Mailloux Vasu D Appanna |
| author_facet | Joseph Lemire Ryan J Mailloux Vasu D Appanna |
| author_sort | Joseph Lemire |
| collection | DOAJ |
| description | Lactate has long been regarded as an end product of anaerobic energy production and its fate in cerebral metabolism has not been precisely delineated. In this report, we demonstrate, for the first time, the ability of a human astrocytic cell line (CCF-STTG1) to consume lactate and to generate ATP via oxidative phosphorylation. (13)C-NMR and HPLC analyses aided in the identification of tricarboxylic acid (TCA) cyle metabolites and ATP in the astrocytic mitochondria incubated with lactate. Oxamate, an inhibitor of lactate dehydrogenase (LDH), abolished mitochondrial lactate consumption. Electrophoretic and fluorescence microscopic analyses helped localize LDH in the mitochondria. Taken together, this study implicates lactate as an important contributor to ATP metabolism in the brain, a finding that may significantly change our notion of how this important organ manipulates its energy budget. |
| first_indexed | 2024-12-12T03:03:49Z |
| format | Article |
| id | doaj.art-7527ad9ffca148b1aca4a8ea09db6411 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T03:03:49Z |
| publishDate | 2008-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-7527ad9ffca148b1aca4a8ea09db64112022-12-22T00:40:34ZengPublic Library of Science (PLoS)PLoS ONE1932-62032008-01-0132e155010.1371/journal.pone.0001550Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1).Joseph LemireRyan J MaillouxVasu D AppannaLactate has long been regarded as an end product of anaerobic energy production and its fate in cerebral metabolism has not been precisely delineated. In this report, we demonstrate, for the first time, the ability of a human astrocytic cell line (CCF-STTG1) to consume lactate and to generate ATP via oxidative phosphorylation. (13)C-NMR and HPLC analyses aided in the identification of tricarboxylic acid (TCA) cyle metabolites and ATP in the astrocytic mitochondria incubated with lactate. Oxamate, an inhibitor of lactate dehydrogenase (LDH), abolished mitochondrial lactate consumption. Electrophoretic and fluorescence microscopic analyses helped localize LDH in the mitochondria. Taken together, this study implicates lactate as an important contributor to ATP metabolism in the brain, a finding that may significantly change our notion of how this important organ manipulates its energy budget.http://europepmc.org/articles/PMC2212712?pdf=render |
| spellingShingle | Joseph Lemire Ryan J Mailloux Vasu D Appanna Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1). PLoS ONE |
| title | Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1). |
| title_full | Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1). |
| title_fullStr | Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1). |
| title_full_unstemmed | Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1). |
| title_short | Mitochondrial lactate dehydrogenase is involved in oxidative-energy metabolism in human astrocytoma cells (CCF-STTG1). |
| title_sort | mitochondrial lactate dehydrogenase is involved in oxidative energy metabolism in human astrocytoma cells ccf sttg1 |
| url | http://europepmc.org/articles/PMC2212712?pdf=render |
| work_keys_str_mv | AT josephlemire mitochondriallactatedehydrogenaseisinvolvedinoxidativeenergymetabolisminhumanastrocytomacellsccfsttg1 AT ryanjmailloux mitochondriallactatedehydrogenaseisinvolvedinoxidativeenergymetabolisminhumanastrocytomacellsccfsttg1 AT vasudappanna mitochondriallactatedehydrogenaseisinvolvedinoxidativeenergymetabolisminhumanastrocytomacellsccfsttg1 |