Stent-based electrode for radiofrequency ablation in the rat esophagus: a preliminary study
Abstract Endoluminal radiofrequency (RF) ablation has been widely used as a safe and effective treatment for Barrett’s esophagus. However, inadequate RF ablation may occur due to insufficient contact between the electrode and target tissues. Herein, a stent-based monopolar RF electrode (SE) was deve...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2022-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-23472-7 |
| _version_ | 1811180239801286656 |
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| author | Dong-Sung Won Yubeen Park Jinsu An Dae Sung Ryu Jeon Min Kang Ji Won Kim Song Hee Kim Chu Hui Zeng Hongbae Kim Hyung-Sik Kim Jung-Hoon Park Sang Soo Lee |
| author_facet | Dong-Sung Won Yubeen Park Jinsu An Dae Sung Ryu Jeon Min Kang Ji Won Kim Song Hee Kim Chu Hui Zeng Hongbae Kim Hyung-Sik Kim Jung-Hoon Park Sang Soo Lee |
| author_sort | Dong-Sung Won |
| collection | DOAJ |
| description | Abstract Endoluminal radiofrequency (RF) ablation has been widely used as a safe and effective treatment for Barrett’s esophagus. However, inadequate RF ablation may occur due to insufficient contact between the electrode and target tissues. Herein, a stent-based monopolar RF electrode (SE) was developed to evenly deliver RF energy to the inner wall of the rat esophagus. The optimal RF parameters were evaluated in the exposed rat esophagus. The temperature in the rat esophagus reached 70 ℃ in 89 s at 30 W, 59 s at 40 W, and 34 s at 50 W. The technical feasibility and efficacy of RF ablation using SE were evaluated based on changes in histological transformation and immunohistochemical parameters of tissues compared at immediately, 1 and 2 weeks after the procedure. The degrees of inflammatory cell infiltration, fibrotic changes, TUNEL, and HSP70 in the RF-ablated rat esophagus were significantly higher than compared with sham control (all p < 0.05). TUNEL-positive deposition gradually decreased, but HSP 70-positive deposition maintained a similar level for 2 weeks. The stent-based RF ablation was technically feasible and effective in evenly inducing thermal damages to the rat esophagus. The RF ablation system using the SE may represent a promising treatment for endoluminal malignancies. |
| first_indexed | 2024-04-11T06:47:55Z |
| format | Article |
| id | doaj.art-75295fc19ce44d2697e2b6e1b7732e0a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T06:47:55Z |
| publishDate | 2022-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-75295fc19ce44d2697e2b6e1b7732e0a2022-12-22T04:39:18ZengNature PortfolioScientific Reports2045-23222022-11-0112111010.1038/s41598-022-23472-7Stent-based electrode for radiofrequency ablation in the rat esophagus: a preliminary studyDong-Sung Won0Yubeen Park1Jinsu An2Dae Sung Ryu3Jeon Min Kang4Ji Won Kim5Song Hee Kim6Chu Hui Zeng7Hongbae Kim8Hyung-Sik Kim9Jung-Hoon Park10Sang Soo Lee11Biomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical CenterBiomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical CenterDepartment of Biomedical Engineering, School of ICT Convergence Engineering, College of Science & Technology, Konkuk UniversityBiomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical CenterBiomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical CenterBiomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical CenterBiomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical CenterBiomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical CenterDepartment of Biosystems & Biomaterials Science and Engineering, Seoul National UniversityDepartment of Mechatronics Engineering, School of ICT Convergence Engineering, College of Science & Technology, Konkuk UniversityBiomedical Engineering Research Center, Asan Institute for Life Sciences, Asan Medical CenterDepartment of Gastroenterology, Asan Medical Center, University of Ulsan College of MedicineAbstract Endoluminal radiofrequency (RF) ablation has been widely used as a safe and effective treatment for Barrett’s esophagus. However, inadequate RF ablation may occur due to insufficient contact between the electrode and target tissues. Herein, a stent-based monopolar RF electrode (SE) was developed to evenly deliver RF energy to the inner wall of the rat esophagus. The optimal RF parameters were evaluated in the exposed rat esophagus. The temperature in the rat esophagus reached 70 ℃ in 89 s at 30 W, 59 s at 40 W, and 34 s at 50 W. The technical feasibility and efficacy of RF ablation using SE were evaluated based on changes in histological transformation and immunohistochemical parameters of tissues compared at immediately, 1 and 2 weeks after the procedure. The degrees of inflammatory cell infiltration, fibrotic changes, TUNEL, and HSP70 in the RF-ablated rat esophagus were significantly higher than compared with sham control (all p < 0.05). TUNEL-positive deposition gradually decreased, but HSP 70-positive deposition maintained a similar level for 2 weeks. The stent-based RF ablation was technically feasible and effective in evenly inducing thermal damages to the rat esophagus. The RF ablation system using the SE may represent a promising treatment for endoluminal malignancies.https://doi.org/10.1038/s41598-022-23472-7 |
| spellingShingle | Dong-Sung Won Yubeen Park Jinsu An Dae Sung Ryu Jeon Min Kang Ji Won Kim Song Hee Kim Chu Hui Zeng Hongbae Kim Hyung-Sik Kim Jung-Hoon Park Sang Soo Lee Stent-based electrode for radiofrequency ablation in the rat esophagus: a preliminary study Scientific Reports |
| title | Stent-based electrode for radiofrequency ablation in the rat esophagus: a preliminary study |
| title_full | Stent-based electrode for radiofrequency ablation in the rat esophagus: a preliminary study |
| title_fullStr | Stent-based electrode for radiofrequency ablation in the rat esophagus: a preliminary study |
| title_full_unstemmed | Stent-based electrode for radiofrequency ablation in the rat esophagus: a preliminary study |
| title_short | Stent-based electrode for radiofrequency ablation in the rat esophagus: a preliminary study |
| title_sort | stent based electrode for radiofrequency ablation in the rat esophagus a preliminary study |
| url | https://doi.org/10.1038/s41598-022-23472-7 |
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