Chromatin associations in Arabidopsis interphase nuclei
The arrangement of chromatin within interphase nuclei seems to be caused by topological constraints and related to gene expression depending on tissue and developmental stage. In yeast and animals it was found that homologous and heterologous chromatin association are required to realize faithful ex...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-11-01
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| Series: | Frontiers in Genetics |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fgene.2014.00389/full |
| _version_ | 1818179469581484032 |
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| author | Veit eSchubert Radek eRudnik Ingo eSchubert |
| author_facet | Veit eSchubert Radek eRudnik Ingo eSchubert |
| author_sort | Veit eSchubert |
| collection | DOAJ |
| description | The arrangement of chromatin within interphase nuclei seems to be caused by topological constraints and related to gene expression depending on tissue and developmental stage. In yeast and animals it was found that homologous and heterologous chromatin association are required to realize faithful expression and DNA repair. To test whether such associations are present in plants we analysed Arabidopsis thaliana interphase nuclei by FISH using probes from different chromosomes. We found that chromatin fibre movement and variable associations, although in general relatively seldom, may occur between euchromatin segments along chromosomes, sometimes even over large distances. The combination of euchromatin segments bearing high or low co-expressing genes did not reveal different association frequencies probably due to adjacent genes of deviating expression patterns.Based on previous data and on FISH analyses presented here, we conclude that the global interphase chromatin organization in A. thaliana is relatively stable, due to the location of its ten centromeres at the nuclear periphery and of the telomeres mainly at the centrally localized nucleolus. Nevertheless, chromatin movement enables a flexible spatial genome arrangement in plant nuclei. |
| first_indexed | 2024-12-11T21:04:22Z |
| format | Article |
| id | doaj.art-752a0d6c333f4b0ab813316fe80ef8d8 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-11T21:04:22Z |
| publishDate | 2014-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-752a0d6c333f4b0ab813316fe80ef8d82022-12-22T00:50:55ZengFrontiers Media S.A.Frontiers in Genetics1664-80212014-11-01510.3389/fgene.2014.00389107489Chromatin associations in Arabidopsis interphase nucleiVeit eSchubert0Radek eRudnik1Ingo eSchubert2IPKIPKIPKThe arrangement of chromatin within interphase nuclei seems to be caused by topological constraints and related to gene expression depending on tissue and developmental stage. In yeast and animals it was found that homologous and heterologous chromatin association are required to realize faithful expression and DNA repair. To test whether such associations are present in plants we analysed Arabidopsis thaliana interphase nuclei by FISH using probes from different chromosomes. We found that chromatin fibre movement and variable associations, although in general relatively seldom, may occur between euchromatin segments along chromosomes, sometimes even over large distances. The combination of euchromatin segments bearing high or low co-expressing genes did not reveal different association frequencies probably due to adjacent genes of deviating expression patterns.Based on previous data and on FISH analyses presented here, we conclude that the global interphase chromatin organization in A. thaliana is relatively stable, due to the location of its ten centromeres at the nuclear periphery and of the telomeres mainly at the centrally localized nucleolus. Nevertheless, chromatin movement enables a flexible spatial genome arrangement in plant nuclei.http://journal.frontiersin.org/Journal/10.3389/fgene.2014.00389/fullArabidopsistranscriptionfishBACExpressionchromosome territory |
| spellingShingle | Veit eSchubert Radek eRudnik Ingo eSchubert Chromatin associations in Arabidopsis interphase nuclei Frontiers in Genetics Arabidopsis transcription fish BAC Expression chromosome territory |
| title | Chromatin associations in Arabidopsis interphase nuclei |
| title_full | Chromatin associations in Arabidopsis interphase nuclei |
| title_fullStr | Chromatin associations in Arabidopsis interphase nuclei |
| title_full_unstemmed | Chromatin associations in Arabidopsis interphase nuclei |
| title_short | Chromatin associations in Arabidopsis interphase nuclei |
| title_sort | chromatin associations in arabidopsis interphase nuclei |
| topic | Arabidopsis transcription fish BAC Expression chromosome territory |
| url | http://journal.frontiersin.org/Journal/10.3389/fgene.2014.00389/full |
| work_keys_str_mv | AT veiteschubert chromatinassociationsinarabidopsisinterphasenuclei AT radekerudnik chromatinassociationsinarabidopsisinterphasenuclei AT ingoeschubert chromatinassociationsinarabidopsisinterphasenuclei |