Development of Subject Specific Finite Element Models of the Mouse Knee Joint for Preclinical Applications
Osteoarthritis is the most common musculoskeletal disabling disease worldwide. Preclinical studies on mice are commonly performed to test new interventions. Finite element (FE) models can be used to study joint mechanics, but usually simplified geometries are used. The aim of this project was to cre...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-10-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.558815/full |
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| author | Sahand Zanjani-Pour Sahand Zanjani-Pour Mario Giorgi Mario Giorgi Enrico Dall'Ara Enrico Dall'Ara |
| author_facet | Sahand Zanjani-Pour Sahand Zanjani-Pour Mario Giorgi Mario Giorgi Enrico Dall'Ara Enrico Dall'Ara |
| author_sort | Sahand Zanjani-Pour |
| collection | DOAJ |
| description | Osteoarthritis is the most common musculoskeletal disabling disease worldwide. Preclinical studies on mice are commonly performed to test new interventions. Finite element (FE) models can be used to study joint mechanics, but usually simplified geometries are used. The aim of this project was to create a realistic subject specific FE model of the mouse knee joint for the assessment of joint mechanical properties. Four different FE models of a C57Bl/6 female mouse knee joint were created based on micro-computed tomography images of specimens stained with phosphotungstic acid in order to include different features: individual cartilage layers with meniscus, individual cartilage layers without meniscus, homogeneous cartilage layers with two different thickness values, and homogeneous cartilage with same thickness for both condyles. They were all analyzed under compressive displacement and the cartilage contact pressure was compared at 0.3 N reaction force. Peak contact pressure in the femur cartilage was 25% lower in the model with subject specific cartilage compared to the simpler model with homogeneous cartilage. A much more homogeneous pressure distribution across the joint was observed in the model with meniscus, with cartilage peak pressure 5–34% lower in the two condyles compared to that with individual cartilage layers. In conclusion, modeling the meniscus and individual cartilage was found to affect the pressure distribution in the mouse knee joint under compressive load and should be included in realistic models for assessing the effect of interventions preclinically. |
| first_indexed | 2024-12-23T10:51:23Z |
| format | Article |
| id | doaj.art-752a45e719764977bdab327ac7710cd7 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-23T10:51:23Z |
| publishDate | 2020-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-752a45e719764977bdab327ac7710cd72022-12-21T17:49:53ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-10-01810.3389/fbioe.2020.558815558815Development of Subject Specific Finite Element Models of the Mouse Knee Joint for Preclinical ApplicationsSahand Zanjani-Pour0Sahand Zanjani-Pour1Mario Giorgi2Mario Giorgi3Enrico Dall'Ara4Enrico Dall'Ara5Department of Oncology and Metabolism, Mellanby Center for Bone Research, University of Sheffield, Sheffield, United KingdomInsigneo Institute for in Silico Medicine, University of Sheffield, Sheffield, United KingdomInsigneo Institute for in Silico Medicine, University of Sheffield, Sheffield, United KingdomCertara Quantitative System Pharmacology, Certara UK Ltd., Simcyp Division, Sheffield, United KingdomDepartment of Oncology and Metabolism, Mellanby Center for Bone Research, University of Sheffield, Sheffield, United KingdomInsigneo Institute for in Silico Medicine, University of Sheffield, Sheffield, United KingdomOsteoarthritis is the most common musculoskeletal disabling disease worldwide. Preclinical studies on mice are commonly performed to test new interventions. Finite element (FE) models can be used to study joint mechanics, but usually simplified geometries are used. The aim of this project was to create a realistic subject specific FE model of the mouse knee joint for the assessment of joint mechanical properties. Four different FE models of a C57Bl/6 female mouse knee joint were created based on micro-computed tomography images of specimens stained with phosphotungstic acid in order to include different features: individual cartilage layers with meniscus, individual cartilage layers without meniscus, homogeneous cartilage layers with two different thickness values, and homogeneous cartilage with same thickness for both condyles. They were all analyzed under compressive displacement and the cartilage contact pressure was compared at 0.3 N reaction force. Peak contact pressure in the femur cartilage was 25% lower in the model with subject specific cartilage compared to the simpler model with homogeneous cartilage. A much more homogeneous pressure distribution across the joint was observed in the model with meniscus, with cartilage peak pressure 5–34% lower in the two condyles compared to that with individual cartilage layers. In conclusion, modeling the meniscus and individual cartilage was found to affect the pressure distribution in the mouse knee joint under compressive load and should be included in realistic models for assessing the effect of interventions preclinically.https://www.frontiersin.org/article/10.3389/fbioe.2020.558815/fullmouse kneefinite elementsubject specificPTA stainingcartilagebone |
| spellingShingle | Sahand Zanjani-Pour Sahand Zanjani-Pour Mario Giorgi Mario Giorgi Enrico Dall'Ara Enrico Dall'Ara Development of Subject Specific Finite Element Models of the Mouse Knee Joint for Preclinical Applications Frontiers in Bioengineering and Biotechnology mouse knee finite element subject specific PTA staining cartilage bone |
| title | Development of Subject Specific Finite Element Models of the Mouse Knee Joint for Preclinical Applications |
| title_full | Development of Subject Specific Finite Element Models of the Mouse Knee Joint for Preclinical Applications |
| title_fullStr | Development of Subject Specific Finite Element Models of the Mouse Knee Joint for Preclinical Applications |
| title_full_unstemmed | Development of Subject Specific Finite Element Models of the Mouse Knee Joint for Preclinical Applications |
| title_short | Development of Subject Specific Finite Element Models of the Mouse Knee Joint for Preclinical Applications |
| title_sort | development of subject specific finite element models of the mouse knee joint for preclinical applications |
| topic | mouse knee finite element subject specific PTA staining cartilage bone |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.558815/full |
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