Evaluation of the adhesion of human dental pulp stem cells to differentendodontic biomaterials before and after setting
Background. Stem cell-based treatment modalities have been potential strategies for tissue regenerationin many conditions. Several studies have evaluated the biologic properties of DPSCs and their efficacyin the treatment of a variety of diseases. The present study was undertaken to evaluate the adh...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Tabriz University of Medical Sciences
2020-06-01
|
| Series: | Journal of Dental Research, Dental Clinics, Dental Prospects |
| Subjects: | |
| Online Access: | https://joddd.tbzmed.ac.ir/PDF/joddd-27274 |
| _version_ | 1828808557068288000 |
|---|---|
| author | Marzie Aghazade Mohammad Samiei Marjan Imani Zahra Aghazadeh Effat Alizadeh Fereshte Rezaie |
| author_facet | Marzie Aghazade Mohammad Samiei Marjan Imani Zahra Aghazadeh Effat Alizadeh Fereshte Rezaie |
| author_sort | Marzie Aghazade |
| collection | DOAJ |
| description | Background. Stem cell-based treatment modalities have been potential strategies for tissue regenerationin many conditions. Several studies have evaluated the biologic properties of DPSCs and their efficacyin the treatment of a variety of diseases. The present study was undertaken to evaluate the adhesionbehavior of DPSCs on different endodontic materials before and after setting. Methods. The crowns of the selected teeth were removed, and the root canals were prepared andobturated with gutta-percha and AH26 sealer. A retrograde cavity was prepared at root ends. Differentmaterials were placed in the cavities. Then the samples were attached to the wells with the use of achemical glue. Dental pulp stem cells were allowed to proliferate to reach a count of 2 million andtransferred to -12well plates in association with a culture medium. Finally, the samples attached to thewells were exposed to the stem cells immersed in the culture medium before and after setting. Thenadhesion of the stem cells was evaluated using SEM. Results. The SEM results showed cellular adhesion in the samples containing CEM cement both beforeand after setting. The samples containing MTA Angelus and ProRoot MTA exhibited cellular adhesionbefore setting, with no cellular adhesion after setting. The samples containing AH26 and MTA Fillapexsealers exhibited cellular adhesion after setting, with no adhesion before setting. The samples containingsimvastatin exhibited no cellular adhesion before setting; this material had dissolved in the culturemedium after setting evaluation. Conclusion. The results of the present study showed that of all the materials tested, CEM cement hadthe highest capacity for dental pulp stem cell adhesion. |
| first_indexed | 2024-12-12T08:42:28Z |
| format | Article |
| id | doaj.art-752baba5a26041e595b651155bc980b1 |
| institution | Directory Open Access Journal |
| issn | 2008-210X 2008-2118 |
| language | English |
| last_indexed | 2024-12-12T08:42:28Z |
| publishDate | 2020-06-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Journal of Dental Research, Dental Clinics, Dental Prospects |
| spelling | doaj.art-752baba5a26041e595b651155bc980b12022-12-22T00:30:43ZengTabriz University of Medical SciencesJournal of Dental Research, Dental Clinics, Dental Prospects2008-210X2008-21182020-06-011429710310.34172/joddd.2020.022joddd-27274Evaluation of the adhesion of human dental pulp stem cells to differentendodontic biomaterials before and after settingMarzie Aghazade0Mohammad Samiei1Marjan Imani2Zahra Aghazadeh3Effat Alizadeh4Fereshte Rezaie5Department of Oral Medicine, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Endodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, IranGeneral Dental Practitioner, Khorram Abad, IranDepartment of Oral Medicine, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, IranDepartment of Medical Biotechnology, Faculty of Advanced Medical Sciences, University of Medical Sciences, Tabriz, IranGeneral Dental Practitioner, Tabriz, IranBackground. Stem cell-based treatment modalities have been potential strategies for tissue regenerationin many conditions. Several studies have evaluated the biologic properties of DPSCs and their efficacyin the treatment of a variety of diseases. The present study was undertaken to evaluate the adhesionbehavior of DPSCs on different endodontic materials before and after setting. Methods. The crowns of the selected teeth were removed, and the root canals were prepared andobturated with gutta-percha and AH26 sealer. A retrograde cavity was prepared at root ends. Differentmaterials were placed in the cavities. Then the samples were attached to the wells with the use of achemical glue. Dental pulp stem cells were allowed to proliferate to reach a count of 2 million andtransferred to -12well plates in association with a culture medium. Finally, the samples attached to thewells were exposed to the stem cells immersed in the culture medium before and after setting. Thenadhesion of the stem cells was evaluated using SEM. Results. The SEM results showed cellular adhesion in the samples containing CEM cement both beforeand after setting. The samples containing MTA Angelus and ProRoot MTA exhibited cellular adhesionbefore setting, with no cellular adhesion after setting. The samples containing AH26 and MTA Fillapexsealers exhibited cellular adhesion after setting, with no adhesion before setting. The samples containingsimvastatin exhibited no cellular adhesion before setting; this material had dissolved in the culturemedium after setting evaluation. Conclusion. The results of the present study showed that of all the materials tested, CEM cement hadthe highest capacity for dental pulp stem cell adhesion.https://joddd.tbzmed.ac.ir/PDF/joddd-27274adult stem cellsbiocompatible materialsmtaregenerative endodontics |
| spellingShingle | Marzie Aghazade Mohammad Samiei Marjan Imani Zahra Aghazadeh Effat Alizadeh Fereshte Rezaie Evaluation of the adhesion of human dental pulp stem cells to differentendodontic biomaterials before and after setting Journal of Dental Research, Dental Clinics, Dental Prospects adult stem cells biocompatible materials mta regenerative endodontics |
| title | Evaluation of the adhesion of human dental pulp stem cells to differentendodontic biomaterials before and after setting |
| title_full | Evaluation of the adhesion of human dental pulp stem cells to differentendodontic biomaterials before and after setting |
| title_fullStr | Evaluation of the adhesion of human dental pulp stem cells to differentendodontic biomaterials before and after setting |
| title_full_unstemmed | Evaluation of the adhesion of human dental pulp stem cells to differentendodontic biomaterials before and after setting |
| title_short | Evaluation of the adhesion of human dental pulp stem cells to differentendodontic biomaterials before and after setting |
| title_sort | evaluation of the adhesion of human dental pulp stem cells to differentendodontic biomaterials before and after setting |
| topic | adult stem cells biocompatible materials mta regenerative endodontics |
| url | https://joddd.tbzmed.ac.ir/PDF/joddd-27274 |
| work_keys_str_mv | AT marzieaghazade evaluationoftheadhesionofhumandentalpulpstemcellstodifferentendodonticbiomaterialsbeforeandaftersetting AT mohammadsamiei evaluationoftheadhesionofhumandentalpulpstemcellstodifferentendodonticbiomaterialsbeforeandaftersetting AT marjanimani evaluationoftheadhesionofhumandentalpulpstemcellstodifferentendodonticbiomaterialsbeforeandaftersetting AT zahraaghazadeh evaluationoftheadhesionofhumandentalpulpstemcellstodifferentendodonticbiomaterialsbeforeandaftersetting AT effatalizadeh evaluationoftheadhesionofhumandentalpulpstemcellstodifferentendodonticbiomaterialsbeforeandaftersetting AT fereshterezaie evaluationoftheadhesionofhumandentalpulpstemcellstodifferentendodonticbiomaterialsbeforeandaftersetting |