Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic Otomastoiditis
Abstract Introduction Chronic otomastoiditis causes pain, otorrhea, and hearing loss resulting from the growth of tissue within the normally hollow mastoid cavity. Objectives In this report, we used a lipidomics approach to profile major mastoid bone and tissue lipids from...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Thieme Revinter Publicações Ltda.
2015-04-01
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| Series: | International Archives of Otorhinolaryngology |
| Subjects: | |
| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0034-1396522 |
| _version_ | 1828484405718417408 |
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| author | Farbod Fazlollahi Kessiri Kongmanas Nongnuj Tanphaichitr Jeffrey Suh Kym Faull Quinton Gopen |
| author_facet | Farbod Fazlollahi Kessiri Kongmanas Nongnuj Tanphaichitr Jeffrey Suh Kym Faull Quinton Gopen |
| author_sort | Farbod Fazlollahi |
| collection | DOAJ |
| description | Abstract
Introduction Chronic otomastoiditis causes pain, otorrhea, and hearing loss resulting from the growth of tissue within the normally hollow mastoid cavity.
Objectives In this report, we used a lipidomics approach to profile major mastoid bone and tissue lipids from patients with and without otomastoiditis.
Methods The bone dust created during mastoidectomy, as well as the mastoid tissue, was analyzed from seven patients. Bone dust was also collected and analyzed in an additional four otologic cases (parotidectomy requiring mastoidectomy). Samples were subjected to a modified Bligh/Dyer lipid extraction, then high-performance thin-layer chromatography (HPTLC), combined gas chromatography/electron impact-mass spectrometry (GC/EI-MS), and flow-injection/electrospray ionization-tandem mass spectrometry (FI/ESI-MSMS). Data were analyzed for identification and profiling of major lipid components.
Results HPTLC revealed the presence of various lipid classes, including phosphatidylcholines, cholesterol, and triacylglycerols. GC/EI-MS analysis revealed the presence of cholesterol and several fatty acids. FI/ESI-MSMS analysis revealed a host of phosphatidylcholines, phosphatidylethanolamines, and cholesteryl esters.
Conclusion We used a lipidomics approach to develop an efficient (both in time and tissue amount) methodology for analysis of these tissues, identify the most abundant and common lipid species, and create a base of knowledge from which more focused endeavors in biomarker discovery can emerge. In an effort toward improved patient categorization and individualized intervention, the ultimate goal of this work is to correlate these lipid molecules to disease state and progression. This is the first reported study of its kind on these tissues. |
| first_indexed | 2024-12-11T08:54:43Z |
| format | Article |
| id | doaj.art-752cc6ac2d1143b68a9adf3b84f04914 |
| institution | Directory Open Access Journal |
| issn | 1809-9777 1809-4864 |
| language | English |
| last_indexed | 2024-12-11T08:54:43Z |
| publishDate | 2015-04-01 |
| publisher | Thieme Revinter Publicações Ltda. |
| record_format | Article |
| series | International Archives of Otorhinolaryngology |
| spelling | doaj.art-752cc6ac2d1143b68a9adf3b84f049142022-12-22T01:13:55ZengThieme Revinter Publicações Ltda.International Archives of Otorhinolaryngology1809-97771809-48642015-04-01190214115010.1055/s-0034-1396522Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic OtomastoiditisFarbod Fazlollahi0Kessiri Kongmanas1Nongnuj Tanphaichitr2Jeffrey Suh3Kym Faull4Quinton Gopen5Department of Psychiatry and Biobehavioral Sciences, UCLA, Los Angeles, United StatesDepartment of Biochemistry/Microbiology/Immunology, University of Ottawa, Ottawa, Ontario, CanadaDepartment of Biochemistry/Microbiology/Immunology, University of Ottawa, Ottawa, Ontario, CanadaDepartment of Otorhinolaryngology–Head and Neck Surgery, UCLA Health System, Los Angeles, California, United StatesDepartment of Psychiatry and Biobehavioral Sciences, UCLA, Los Angeles, United StatesDepartment of Otorhinolaryngology–Head and Neck Surgery, UCLA Health System, Los Angeles, California, United StatesAbstract Introduction Chronic otomastoiditis causes pain, otorrhea, and hearing loss resulting from the growth of tissue within the normally hollow mastoid cavity. Objectives In this report, we used a lipidomics approach to profile major mastoid bone and tissue lipids from patients with and without otomastoiditis. Methods The bone dust created during mastoidectomy, as well as the mastoid tissue, was analyzed from seven patients. Bone dust was also collected and analyzed in an additional four otologic cases (parotidectomy requiring mastoidectomy). Samples were subjected to a modified Bligh/Dyer lipid extraction, then high-performance thin-layer chromatography (HPTLC), combined gas chromatography/electron impact-mass spectrometry (GC/EI-MS), and flow-injection/electrospray ionization-tandem mass spectrometry (FI/ESI-MSMS). Data were analyzed for identification and profiling of major lipid components. Results HPTLC revealed the presence of various lipid classes, including phosphatidylcholines, cholesterol, and triacylglycerols. GC/EI-MS analysis revealed the presence of cholesterol and several fatty acids. FI/ESI-MSMS analysis revealed a host of phosphatidylcholines, phosphatidylethanolamines, and cholesteryl esters. Conclusion We used a lipidomics approach to develop an efficient (both in time and tissue amount) methodology for analysis of these tissues, identify the most abundant and common lipid species, and create a base of knowledge from which more focused endeavors in biomarker discovery can emerge. In an effort toward improved patient categorization and individualized intervention, the ultimate goal of this work is to correlate these lipid molecules to disease state and progression. This is the first reported study of its kind on these tissues.http://www.thieme-connect.de/DOI/DOI?10.1055/s-0034-1396522mastoiditislipid metabolismmass spectrometryflow injection analysis |
| spellingShingle | Farbod Fazlollahi Kessiri Kongmanas Nongnuj Tanphaichitr Jeffrey Suh Kym Faull Quinton Gopen Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic Otomastoiditis International Archives of Otorhinolaryngology mastoiditis lipid metabolism mass spectrometry flow injection analysis |
| title | Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic Otomastoiditis |
| title_full | Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic Otomastoiditis |
| title_fullStr | Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic Otomastoiditis |
| title_full_unstemmed | Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic Otomastoiditis |
| title_short | Lipidomic Profiling of Mastoid Bone and Tissue from Patients with Chronic Otomastoiditis |
| title_sort | lipidomic profiling of mastoid bone and tissue from patients with chronic otomastoiditis |
| topic | mastoiditis lipid metabolism mass spectrometry flow injection analysis |
| url | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0034-1396522 |
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