Enhancing In Vitro Production of the Tree Fern <i>Cyathea delgadii</i> and Modifying Secondary Metabolite Profiles by LED Lighting
The tree ferns are an important component of tropical forests. In view of this, the enhancement of in vitro production of these plants is needed. Thus, the effect of different light-emitting diodes (LEDs) as well as control fluorescent lamps (Fl) and a 3-week-long period of darkness at the beginning...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Cells |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4409/11/3/486 |
| _version_ | 1797488514609184768 |
|---|---|
| author | Wojciech Tomaszewicz Monika Cioć Katarzyna Dos Santos Szewczyk Małgorzata Grzyb Wioleta Pietrzak Bożena Pawłowska Anna Mikuła |
| author_facet | Wojciech Tomaszewicz Monika Cioć Katarzyna Dos Santos Szewczyk Małgorzata Grzyb Wioleta Pietrzak Bożena Pawłowska Anna Mikuła |
| author_sort | Wojciech Tomaszewicz |
| collection | DOAJ |
| description | The tree ferns are an important component of tropical forests. In view of this, the enhancement of in vitro production of these plants is needed. Thus, the effect of different light-emitting diodes (LEDs) as well as control fluorescent lamps (Fl) and a 3-week-long period of darkness at the beginning of in vitro culture on micropropagation of the tree fern <i>Cyathea delgadii</i> Sternb. was analysed. Moreover, the photosynthetic pigment content and secondary metabolite profiles were estimated. The period of darkness contributed to a high production of somatic embryo-derived sporophytes and a low production of gametophytes. The formation of new sporophytes was stimulated by RBY (35% red, 15% blue, and 50% yellow) and B (100% blue) lights when the stipe explants or whole young sporophytes were used in the culture, respectively. The elongation of the roots and leaves was stimulated by RBfR light (35% red, 15% blue, and 50% far red), while root production increased under RBY light. The RB (70% red and 30% blue) and B lights stimulated the accumulation of chlorophyll better than Fl light. The most abundant metabolite found in the plant extracts was <i>trans</i>-5-<i>O</i>-caffeoylquinic acid (1.013 µg/mg of dry weight). The extract obtained from plants growing in a greenhouse had the best antioxidant activity. |
| first_indexed | 2024-03-10T00:03:18Z |
| format | Article |
| id | doaj.art-752daeec4bcc49c298819ea3c0a749a0 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T00:03:18Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-752daeec4bcc49c298819ea3c0a749a02023-11-23T16:12:45ZengMDPI AGCells2073-44092022-01-0111348610.3390/cells11030486Enhancing In Vitro Production of the Tree Fern <i>Cyathea delgadii</i> and Modifying Secondary Metabolite Profiles by LED LightingWojciech Tomaszewicz0Monika Cioć1Katarzyna Dos Santos Szewczyk2Małgorzata Grzyb3Wioleta Pietrzak4Bożena Pawłowska5Anna Mikuła6Center for Biological Diversity Conservation in Powsin—Polish Academy of Sciences Botanical Garden, Prawdziwka 2, 02-973 Warsaw, PolandDepartment of Ornamental Plants and Garden Art, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Kraków, PolandDepartment of Pharmaceutical Botany, Medical University of Lublin, Chodźki 1, 20-093 Lublin, PolandCenter for Biological Diversity Conservation in Powsin—Polish Academy of Sciences Botanical Garden, Prawdziwka 2, 02-973 Warsaw, PolandDepartment of Pharmaceutical Botany, Medical University of Lublin, Chodźki 1, 20-093 Lublin, PolandDepartment of Ornamental Plants and Garden Art, University of Agriculture in Krakow, 29 Listopada 54, 31-425 Kraków, PolandCenter for Biological Diversity Conservation in Powsin—Polish Academy of Sciences Botanical Garden, Prawdziwka 2, 02-973 Warsaw, PolandThe tree ferns are an important component of tropical forests. In view of this, the enhancement of in vitro production of these plants is needed. Thus, the effect of different light-emitting diodes (LEDs) as well as control fluorescent lamps (Fl) and a 3-week-long period of darkness at the beginning of in vitro culture on micropropagation of the tree fern <i>Cyathea delgadii</i> Sternb. was analysed. Moreover, the photosynthetic pigment content and secondary metabolite profiles were estimated. The period of darkness contributed to a high production of somatic embryo-derived sporophytes and a low production of gametophytes. The formation of new sporophytes was stimulated by RBY (35% red, 15% blue, and 50% yellow) and B (100% blue) lights when the stipe explants or whole young sporophytes were used in the culture, respectively. The elongation of the roots and leaves was stimulated by RBfR light (35% red, 15% blue, and 50% far red), while root production increased under RBY light. The RB (70% red and 30% blue) and B lights stimulated the accumulation of chlorophyll better than Fl light. The most abundant metabolite found in the plant extracts was <i>trans</i>-5-<i>O</i>-caffeoylquinic acid (1.013 µg/mg of dry weight). The extract obtained from plants growing in a greenhouse had the best antioxidant activity.https://www.mdpi.com/2073-4409/11/3/486light-emitting diodesomatic embryogenesisphotosynthetic pigmentsphenolic acidsantioxidant activitytree fern |
| spellingShingle | Wojciech Tomaszewicz Monika Cioć Katarzyna Dos Santos Szewczyk Małgorzata Grzyb Wioleta Pietrzak Bożena Pawłowska Anna Mikuła Enhancing In Vitro Production of the Tree Fern <i>Cyathea delgadii</i> and Modifying Secondary Metabolite Profiles by LED Lighting Cells light-emitting diode somatic embryogenesis photosynthetic pigments phenolic acids antioxidant activity tree fern |
| title | Enhancing In Vitro Production of the Tree Fern <i>Cyathea delgadii</i> and Modifying Secondary Metabolite Profiles by LED Lighting |
| title_full | Enhancing In Vitro Production of the Tree Fern <i>Cyathea delgadii</i> and Modifying Secondary Metabolite Profiles by LED Lighting |
| title_fullStr | Enhancing In Vitro Production of the Tree Fern <i>Cyathea delgadii</i> and Modifying Secondary Metabolite Profiles by LED Lighting |
| title_full_unstemmed | Enhancing In Vitro Production of the Tree Fern <i>Cyathea delgadii</i> and Modifying Secondary Metabolite Profiles by LED Lighting |
| title_short | Enhancing In Vitro Production of the Tree Fern <i>Cyathea delgadii</i> and Modifying Secondary Metabolite Profiles by LED Lighting |
| title_sort | enhancing in vitro production of the tree fern i cyathea delgadii i and modifying secondary metabolite profiles by led lighting |
| topic | light-emitting diode somatic embryogenesis photosynthetic pigments phenolic acids antioxidant activity tree fern |
| url | https://www.mdpi.com/2073-4409/11/3/486 |
| work_keys_str_mv | AT wojciechtomaszewicz enhancinginvitroproductionofthetreefernicyatheadelgadiiiandmodifyingsecondarymetaboliteprofilesbyledlighting AT monikacioc enhancinginvitroproductionofthetreefernicyatheadelgadiiiandmodifyingsecondarymetaboliteprofilesbyledlighting AT katarzynadossantosszewczyk enhancinginvitroproductionofthetreefernicyatheadelgadiiiandmodifyingsecondarymetaboliteprofilesbyledlighting AT małgorzatagrzyb enhancinginvitroproductionofthetreefernicyatheadelgadiiiandmodifyingsecondarymetaboliteprofilesbyledlighting AT wioletapietrzak enhancinginvitroproductionofthetreefernicyatheadelgadiiiandmodifyingsecondarymetaboliteprofilesbyledlighting AT bozenapawłowska enhancinginvitroproductionofthetreefernicyatheadelgadiiiandmodifyingsecondarymetaboliteprofilesbyledlighting AT annamikuła enhancinginvitroproductionofthetreefernicyatheadelgadiiiandmodifyingsecondarymetaboliteprofilesbyledlighting |