Characterization of Specific IgE Antibody Related to Antigen 5 of Echinococcus granulosus
Background: Anaphylactic reactions, such as urticaria, edema, respiratory symptoms, and anaphylactic shock often complicate the course of Cystic Echinococcosis (CE). Methods: To investigate the role of the IgE immunoreactive antigen 5 (Ag 5) in the sero-positive patients with CE, we determined N-ter...
| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | English |
| Publicado: |
Tehran University of Medical Sciences
2007-03-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Acceso en liña: | https://ijph.tums.ac.ir/index.php/ijph/article/view/2129 |
| _version_ | 1831677018778894336 |
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| author | AR Khabiri F Bagheri MR Siavashi M Assmar |
| author_facet | AR Khabiri F Bagheri MR Siavashi M Assmar |
| author_sort | AR Khabiri |
| collection | DOAJ |
| description | Background: Anaphylactic reactions, such as urticaria, edema, respiratory symptoms, and anaphylactic shock often complicate the course of Cystic Echinococcosis (CE). Methods: To investigate the role of the IgE immunoreactive antigen 5 (Ag 5) in the sero-positive patients with CE, we determined N-terminal of 57 kDa subunit of Ag5 responsible for IgE and C-terminal of this active antigen related to induction of IgG specifically. Results: Immunoblotting analysis showed that specific IgE to 57-kDa subunit related to inter-chain disulphide band of two 22 kDa and 38-kDa component of Ag5 and conformational epitope on this subunits. In addition, since the 57 kDa component arise from the removal of the C-terminal portion of 22 kDa subunit of Ag5, thus IgE specifically recognized N-terminal of 22 kDa subunit which remain bounds to the other component, whereas IgG reacted with C-terminal of 38 kDa component of Ag5. Conclusion: Recognition of the specific binding site on the 57 kDa subunit of Ag5 could leads to understanding the mechanism regulating IgE/IgG production in some immune circumstances that IgE tends to some dominate, whereas in other IgG predominates. |
| first_indexed | 2024-12-20T04:34:29Z |
| format | Article |
| id | doaj.art-752e05f455d94c20baff8e6db5e294d5 |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-20T04:34:29Z |
| publishDate | 2007-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-752e05f455d94c20baff8e6db5e294d52022-12-21T19:53:18ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932007-03-01361Characterization of Specific IgE Antibody Related to Antigen 5 of Echinococcus granulosus AR Khabiri0 F Bagheri1 MR Siavashi2 M Assmar3 Background: Anaphylactic reactions, such as urticaria, edema, respiratory symptoms, and anaphylactic shock often complicate the course of Cystic Echinococcosis (CE). Methods: To investigate the role of the IgE immunoreactive antigen 5 (Ag 5) in the sero-positive patients with CE, we determined N-terminal of 57 kDa subunit of Ag5 responsible for IgE and C-terminal of this active antigen related to induction of IgG specifically. Results: Immunoblotting analysis showed that specific IgE to 57-kDa subunit related to inter-chain disulphide band of two 22 kDa and 38-kDa component of Ag5 and conformational epitope on this subunits. In addition, since the 57 kDa component arise from the removal of the C-terminal portion of 22 kDa subunit of Ag5, thus IgE specifically recognized N-terminal of 22 kDa subunit which remain bounds to the other component, whereas IgG reacted with C-terminal of 38 kDa component of Ag5. Conclusion: Recognition of the specific binding site on the 57 kDa subunit of Ag5 could leads to understanding the mechanism regulating IgE/IgG production in some immune circumstances that IgE tends to some dominate, whereas in other IgG predominates.https://ijph.tums.ac.ir/index.php/ijph/article/view/2129Cystic EchinococcosisAntigen 5 |
| spellingShingle | AR Khabiri F Bagheri MR Siavashi M Assmar Characterization of Specific IgE Antibody Related to Antigen 5 of Echinococcus granulosus Iranian Journal of Public Health Cystic Echinococcosis Antigen 5 |
| title | Characterization of Specific IgE Antibody Related to Antigen 5 of Echinococcus granulosus |
| title_full | Characterization of Specific IgE Antibody Related to Antigen 5 of Echinococcus granulosus |
| title_fullStr | Characterization of Specific IgE Antibody Related to Antigen 5 of Echinococcus granulosus |
| title_full_unstemmed | Characterization of Specific IgE Antibody Related to Antigen 5 of Echinococcus granulosus |
| title_short | Characterization of Specific IgE Antibody Related to Antigen 5 of Echinococcus granulosus |
| title_sort | characterization of specific ige antibody related to antigen 5 of echinococcus granulosus |
| topic | Cystic Echinococcosis Antigen 5 |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/2129 |
| work_keys_str_mv | AT arkhabiri characterizationofspecificigeantibodyrelatedtoantigen5ofechinococcusgranulosus AT fbagheri characterizationofspecificigeantibodyrelatedtoantigen5ofechinococcusgranulosus AT mrsiavashi characterizationofspecificigeantibodyrelatedtoantigen5ofechinococcusgranulosus AT massmar characterizationofspecificigeantibodyrelatedtoantigen5ofechinococcusgranulosus |