Sparse and compositionally robust inference of microbial ecological networks.
16S ribosomal RNA (rRNA) gene and other environmental sequencing techniques provide snapshots of microbial communities, revealing phylogeny and the abundances of microbial populations across diverse ecosystems. While changes in microbial community structure are demonstrably associated with certain e...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-05-01
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| Series: | PLoS Computational Biology |
| Online Access: | http://europepmc.org/articles/PMC4423992?pdf=render |
| _version_ | 1818042576398188544 |
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| author | Zachary D Kurtz Christian L Müller Emily R Miraldi Dan R Littman Martin J Blaser Richard A Bonneau |
| author_facet | Zachary D Kurtz Christian L Müller Emily R Miraldi Dan R Littman Martin J Blaser Richard A Bonneau |
| author_sort | Zachary D Kurtz |
| collection | DOAJ |
| description | 16S ribosomal RNA (rRNA) gene and other environmental sequencing techniques provide snapshots of microbial communities, revealing phylogeny and the abundances of microbial populations across diverse ecosystems. While changes in microbial community structure are demonstrably associated with certain environmental conditions (from metabolic and immunological health in mammals to ecological stability in soils and oceans), identification of underlying mechanisms requires new statistical tools, as these datasets present several technical challenges. First, the abundances of microbial operational taxonomic units (OTUs) from amplicon-based datasets are compositional. Counts are normalized to the total number of counts in the sample. Thus, microbial abundances are not independent, and traditional statistical metrics (e.g., correlation) for the detection of OTU-OTU relationships can lead to spurious results. Secondly, microbial sequencing-based studies typically measure hundreds of OTUs on only tens to hundreds of samples; thus, inference of OTU-OTU association networks is severely under-powered, and additional information (or assumptions) are required for accurate inference. Here, we present SPIEC-EASI (SParse InversE Covariance Estimation for Ecological Association Inference), a statistical method for the inference of microbial ecological networks from amplicon sequencing datasets that addresses both of these issues. SPIEC-EASI combines data transformations developed for compositional data analysis with a graphical model inference framework that assumes the underlying ecological association network is sparse. To reconstruct the network, SPIEC-EASI relies on algorithms for sparse neighborhood and inverse covariance selection. To provide a synthetic benchmark in the absence of an experimentally validated gold-standard network, SPIEC-EASI is accompanied by a set of computational tools to generate OTU count data from a set of diverse underlying network topologies. SPIEC-EASI outperforms state-of-the-art methods to recover edges and network properties on synthetic data under a variety of scenarios. SPIEC-EASI also reproducibly predicts previously unknown microbial associations using data from the American Gut project. |
| first_indexed | 2024-12-10T08:48:31Z |
| format | Article |
| id | doaj.art-752f5ef6eeac4cfc919259032b5c8211 |
| institution | Directory Open Access Journal |
| issn | 1553-734X 1553-7358 |
| language | English |
| last_indexed | 2024-12-10T08:48:31Z |
| publishDate | 2015-05-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Computational Biology |
| spelling | doaj.art-752f5ef6eeac4cfc919259032b5c82112022-12-22T01:55:40ZengPublic Library of Science (PLoS)PLoS Computational Biology1553-734X1553-73582015-05-01115e100422610.1371/journal.pcbi.1004226Sparse and compositionally robust inference of microbial ecological networks.Zachary D KurtzChristian L MüllerEmily R MiraldiDan R LittmanMartin J BlaserRichard A Bonneau16S ribosomal RNA (rRNA) gene and other environmental sequencing techniques provide snapshots of microbial communities, revealing phylogeny and the abundances of microbial populations across diverse ecosystems. While changes in microbial community structure are demonstrably associated with certain environmental conditions (from metabolic and immunological health in mammals to ecological stability in soils and oceans), identification of underlying mechanisms requires new statistical tools, as these datasets present several technical challenges. First, the abundances of microbial operational taxonomic units (OTUs) from amplicon-based datasets are compositional. Counts are normalized to the total number of counts in the sample. Thus, microbial abundances are not independent, and traditional statistical metrics (e.g., correlation) for the detection of OTU-OTU relationships can lead to spurious results. Secondly, microbial sequencing-based studies typically measure hundreds of OTUs on only tens to hundreds of samples; thus, inference of OTU-OTU association networks is severely under-powered, and additional information (or assumptions) are required for accurate inference. Here, we present SPIEC-EASI (SParse InversE Covariance Estimation for Ecological Association Inference), a statistical method for the inference of microbial ecological networks from amplicon sequencing datasets that addresses both of these issues. SPIEC-EASI combines data transformations developed for compositional data analysis with a graphical model inference framework that assumes the underlying ecological association network is sparse. To reconstruct the network, SPIEC-EASI relies on algorithms for sparse neighborhood and inverse covariance selection. To provide a synthetic benchmark in the absence of an experimentally validated gold-standard network, SPIEC-EASI is accompanied by a set of computational tools to generate OTU count data from a set of diverse underlying network topologies. SPIEC-EASI outperforms state-of-the-art methods to recover edges and network properties on synthetic data under a variety of scenarios. SPIEC-EASI also reproducibly predicts previously unknown microbial associations using data from the American Gut project.http://europepmc.org/articles/PMC4423992?pdf=render |
| spellingShingle | Zachary D Kurtz Christian L Müller Emily R Miraldi Dan R Littman Martin J Blaser Richard A Bonneau Sparse and compositionally robust inference of microbial ecological networks. PLoS Computational Biology |
| title | Sparse and compositionally robust inference of microbial ecological networks. |
| title_full | Sparse and compositionally robust inference of microbial ecological networks. |
| title_fullStr | Sparse and compositionally robust inference of microbial ecological networks. |
| title_full_unstemmed | Sparse and compositionally robust inference of microbial ecological networks. |
| title_short | Sparse and compositionally robust inference of microbial ecological networks. |
| title_sort | sparse and compositionally robust inference of microbial ecological networks |
| url | http://europepmc.org/articles/PMC4423992?pdf=render |
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