| Summary: | <i>Eugenia uniflora linnaeus</i>, known as Brazilian cherry, is widely distributed in Brazil, Argentina, Uruguay, and Paraguay. <i>E. uniflora</i> L. extracts contain phenolic compounds, such as flavonoids, tannins, triterpenes, and sesquiterpenes. The antimicrobial action of essential oils has been attributed to their compositions of bioactive compounds, such as sesquiterpenes. In this paper, the fruit extract of <i>E. uniflora</i> was used to synthesize silver and gold nanoparticles. The nanoparticles were characterized by UV–Vis, transmission electron microscopy, elemental analysis, FTIR, and Zeta potential measurement. The silver and gold nanoparticles prepared with fruit extracts presented sizes of ~32 nm and 11 nm (diameter), respectively, and Zeta potentials of −22 mV and −14 mV. The antimicrobial tests were performed with Gram-negative and Gram-positive bacteria and <i>Candida albicans</i>. The growth inhibition of <i>Eu</i>AgNPs prepared with and without photoreduction showed the important functional groups in the antimicrobial activity.
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