Erythromycin Treatment of <i>Brassica campestris</i> Seedlings Impacts the Photosynthetic and Protein Synthesis Pathways
Erythromycin (Ery) is a commonly used veterinary drug that prevents infections and promotes the growth of farm animals. Ery is often detected in agricultural fields due to the effects of manure application in the ecosystem. However, there is a lack of information on Ery toxicity in crops. In this st...
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MDPI AG
2020-11-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/10/12/311 |
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| author | Young-Eun Yoon Hyun Min Cho Dong-won Bae Sung Joong Lee Hyeonji Choe Min Chul Kim Mi Sun Cheong Yong Bok Lee |
| author_facet | Young-Eun Yoon Hyun Min Cho Dong-won Bae Sung Joong Lee Hyeonji Choe Min Chul Kim Mi Sun Cheong Yong Bok Lee |
| author_sort | Young-Eun Yoon |
| collection | DOAJ |
| description | Erythromycin (Ery) is a commonly used veterinary drug that prevents infections and promotes the growth of farm animals. Ery is often detected in agricultural fields due to the effects of manure application in the ecosystem. However, there is a lack of information on Ery toxicity in crops. In this study, we performed a comparative proteomic analysis to identify the molecular mechanisms of Ery toxicity during seedling growth based on our observation of a decrease in chlorophyll (Chl) contents using <i>Brassica campestris</i>. A total of 452 differentially abundant proteins (DAPs) were identified including a ribulose-1,5-bisphosphate carboxylase (RuBisCO). The proteomic analysis according to gene ontology (GO) classification revealed that many of these DAPs responding to Ery treatment functioned in a cellular process and a metabolic process. The molecular function analysis showed that DAPs classified within catalytic activity were predominantly changed by Ery, including metabolite interconversion enzyme and protein modifying enzyme. An analysis of functional pathways using MapMan revealed that many photosynthesis components were downregulated, whereas many protein biosynthesis components were upregulated. A good relationship was observed between protein and transcript abundance in a photosynthetic pathway, as determined by qPCR analysis. These combined results suggest that Ery affects plant physiological activity by downregulating protein abundance in the photosynthetic pathway. |
| first_indexed | 2024-03-10T14:34:02Z |
| format | Article |
| id | doaj.art-753244646c5d43cb87af5e963c2ebe98 |
| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-10T14:34:02Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-753244646c5d43cb87af5e963c2ebe982023-11-20T22:22:43ZengMDPI AGLife2075-17292020-11-01101231110.3390/life10120311Erythromycin Treatment of <i>Brassica campestris</i> Seedlings Impacts the Photosynthetic and Protein Synthesis PathwaysYoung-Eun Yoon0Hyun Min Cho1Dong-won Bae2Sung Joong Lee3Hyeonji Choe4Min Chul Kim5Mi Sun Cheong6Yong Bok Lee7Division of Applied Life Science (BK21four), Gyeongsang National University, Jinju-daero 501, Jinju 52665, KoreaDivision of Applied Life Science (BK21four), Gyeongsang National University, Jinju-daero 501, Jinju 52665, KoreaCenter for Research Facilities, Gyeongsang National University, Jinju-daero 501, Jinju 52665, KoreaInstitute of Agriculture & Life Science, Gyeongsang National University, Jinju-daero 501, Jinju 52665, KoreaDivision of Applied Life Science (BK21four), Gyeongsang National University, Jinju-daero 501, Jinju 52665, KoreaDivision of Applied Life Science (BK21four), Gyeongsang National University, Jinju-daero 501, Jinju 52665, KoreaDivision of Applied Life Science (BK21four), Gyeongsang National University, Jinju-daero 501, Jinju 52665, KoreaDivision of Applied Life Science (BK21four), Gyeongsang National University, Jinju-daero 501, Jinju 52665, KoreaErythromycin (Ery) is a commonly used veterinary drug that prevents infections and promotes the growth of farm animals. Ery is often detected in agricultural fields due to the effects of manure application in the ecosystem. However, there is a lack of information on Ery toxicity in crops. In this study, we performed a comparative proteomic analysis to identify the molecular mechanisms of Ery toxicity during seedling growth based on our observation of a decrease in chlorophyll (Chl) contents using <i>Brassica campestris</i>. A total of 452 differentially abundant proteins (DAPs) were identified including a ribulose-1,5-bisphosphate carboxylase (RuBisCO). The proteomic analysis according to gene ontology (GO) classification revealed that many of these DAPs responding to Ery treatment functioned in a cellular process and a metabolic process. The molecular function analysis showed that DAPs classified within catalytic activity were predominantly changed by Ery, including metabolite interconversion enzyme and protein modifying enzyme. An analysis of functional pathways using MapMan revealed that many photosynthesis components were downregulated, whereas many protein biosynthesis components were upregulated. A good relationship was observed between protein and transcript abundance in a photosynthetic pathway, as determined by qPCR analysis. These combined results suggest that Ery affects plant physiological activity by downregulating protein abundance in the photosynthetic pathway.https://www.mdpi.com/2075-1729/10/12/311antibioticserythromycin (Ery)<i>Brassica campestris</i>proteomicsphotosynthesisprotein synthesis |
| spellingShingle | Young-Eun Yoon Hyun Min Cho Dong-won Bae Sung Joong Lee Hyeonji Choe Min Chul Kim Mi Sun Cheong Yong Bok Lee Erythromycin Treatment of <i>Brassica campestris</i> Seedlings Impacts the Photosynthetic and Protein Synthesis Pathways Life antibiotics erythromycin (Ery) <i>Brassica campestris</i> proteomics photosynthesis protein synthesis |
| title | Erythromycin Treatment of <i>Brassica campestris</i> Seedlings Impacts the Photosynthetic and Protein Synthesis Pathways |
| title_full | Erythromycin Treatment of <i>Brassica campestris</i> Seedlings Impacts the Photosynthetic and Protein Synthesis Pathways |
| title_fullStr | Erythromycin Treatment of <i>Brassica campestris</i> Seedlings Impacts the Photosynthetic and Protein Synthesis Pathways |
| title_full_unstemmed | Erythromycin Treatment of <i>Brassica campestris</i> Seedlings Impacts the Photosynthetic and Protein Synthesis Pathways |
| title_short | Erythromycin Treatment of <i>Brassica campestris</i> Seedlings Impacts the Photosynthetic and Protein Synthesis Pathways |
| title_sort | erythromycin treatment of i brassica campestris i seedlings impacts the photosynthetic and protein synthesis pathways |
| topic | antibiotics erythromycin (Ery) <i>Brassica campestris</i> proteomics photosynthesis protein synthesis |
| url | https://www.mdpi.com/2075-1729/10/12/311 |
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