Characterization of the Sphingolipidome of the Peri-Infarct Tissue during Hemorrhagic Transformation in a Mouse Model of Cerebral Ischemia
Background: Cardiovascular diseases like stroke cause changes to sphingolipid mediators like sphingosine 1-phosphate (S1P) or its ceramide analogs, which bear the potential to either alleviate or exacerbate the neurological damage. Therefore, the precise identification of alterations within the sphi...
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| Format: | Article |
| Language: | English |
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IMR Press
2022-09-01
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| Series: | Journal of Integrative Neuroscience |
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| Online Access: | https://www.imrpress.com/journal/JIN/21/6/10.31083/j.jin2106161 |
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| author | Alexandra Lucaciu Sandra Trautmann Dominique Thomas Karsten Lachner Robert Brunkhorst Julien Subburayalu |
| author_facet | Alexandra Lucaciu Sandra Trautmann Dominique Thomas Karsten Lachner Robert Brunkhorst Julien Subburayalu |
| author_sort | Alexandra Lucaciu |
| collection | DOAJ |
| description | Background: Cardiovascular diseases like stroke cause changes to sphingolipid mediators like sphingosine 1-phosphate (S1P) or its ceramide analogs, which bear the potential to either alleviate or exacerbate the neurological damage. Therefore, the precise identification of alterations within the sphingolipidome during ischemic stroke (IS) and hemorrhagic transformation (HT) harbors a putative therapeutic potential to orchestrate local and systemic immunomodulatory processes. Due to the scarcity of research in this field, we aimed to characterize the sphingolipidome in IS and HT. Methods: C57BL/6 mice underwent middle cerebral artery occlusion (MCAO) and specimens of the peri-infarct tissue were taken for sphingolipid profiling. Results: Ischemic stroke resulted in reduced S1P whilst ceramides were elevated six hours post ischemia onset. However, these differences were nearly revoked at 24 hours post ischemia onset. Moreover, the topmost S1P and ceramide levels were linked to the presence of HT after MCAO. In this study we show the characterization of the sphingolipidomic landscape of the peri-infarct tissue after ischemic stroke and HT. Especially, highest values of S1P, C 18 lactosylceramide, C 18 glucosylceramide, and C 24:1 ceramide were nearly entirely expressed by mice with HT. Conclusions: Our results warrant further investigations into the immunomodulatory consequences of altered sphingolipid species for the development of HT after IS. |
| first_indexed | 2024-04-11T07:39:39Z |
| format | Article |
| id | doaj.art-753a03e7e4664216acbb3aa22f10497e |
| institution | Directory Open Access Journal |
| issn | 0219-6352 |
| language | English |
| last_indexed | 2024-04-11T07:39:39Z |
| publishDate | 2022-09-01 |
| publisher | IMR Press |
| record_format | Article |
| series | Journal of Integrative Neuroscience |
| spelling | doaj.art-753a03e7e4664216acbb3aa22f10497e2022-12-22T04:36:36ZengIMR PressJournal of Integrative Neuroscience0219-63522022-09-0121616110.31083/j.jin2106161S0219-6352(22)00397-7Characterization of the Sphingolipidome of the Peri-Infarct Tissue during Hemorrhagic Transformation in a Mouse Model of Cerebral IschemiaAlexandra Lucaciu0Sandra Trautmann1Dominique Thomas2Karsten Lachner3Robert Brunkhorst4Julien Subburayalu5Department of Neurology, Goethe University Frankfurt, 60590 Frankfurt am Main, GermanyInstitute of Clinical Pharmacology, Pharmazentrum Frankfurt, Goethe University Frankfurt, 60590 Frankfurt am Main, GermanyInstitute of Clinical Pharmacology, Pharmazentrum Frankfurt, Goethe University Frankfurt, 60590 Frankfurt am Main, GermanyDepartment of Neuroradiology, Goethe University Frankfurt, 60590 Frankfurt am Main, GermanyDepartment of Neurology, RWTH Aachen University, 52074 Aachen, GermanyDepartment of Medicine, University of Cambridge, CB2 0QQ Cambridge, UKBackground: Cardiovascular diseases like stroke cause changes to sphingolipid mediators like sphingosine 1-phosphate (S1P) or its ceramide analogs, which bear the potential to either alleviate or exacerbate the neurological damage. Therefore, the precise identification of alterations within the sphingolipidome during ischemic stroke (IS) and hemorrhagic transformation (HT) harbors a putative therapeutic potential to orchestrate local and systemic immunomodulatory processes. Due to the scarcity of research in this field, we aimed to characterize the sphingolipidome in IS and HT. Methods: C57BL/6 mice underwent middle cerebral artery occlusion (MCAO) and specimens of the peri-infarct tissue were taken for sphingolipid profiling. Results: Ischemic stroke resulted in reduced S1P whilst ceramides were elevated six hours post ischemia onset. However, these differences were nearly revoked at 24 hours post ischemia onset. Moreover, the topmost S1P and ceramide levels were linked to the presence of HT after MCAO. In this study we show the characterization of the sphingolipidomic landscape of the peri-infarct tissue after ischemic stroke and HT. Especially, highest values of S1P, C 18 lactosylceramide, C 18 glucosylceramide, and C 24:1 ceramide were nearly entirely expressed by mice with HT. Conclusions: Our results warrant further investigations into the immunomodulatory consequences of altered sphingolipid species for the development of HT after IS.https://www.imrpress.com/journal/JIN/21/6/10.31083/j.jin2106161sphingosine 1-phosphateceramides1p 1ischemic strokehemorrhagic transformationsphingolipid profilingmcaoc57bl/6 |
| spellingShingle | Alexandra Lucaciu Sandra Trautmann Dominique Thomas Karsten Lachner Robert Brunkhorst Julien Subburayalu Characterization of the Sphingolipidome of the Peri-Infarct Tissue during Hemorrhagic Transformation in a Mouse Model of Cerebral Ischemia Journal of Integrative Neuroscience sphingosine 1-phosphate ceramide s1p 1 ischemic stroke hemorrhagic transformation sphingolipid profiling mcao c57bl/6 |
| title | Characterization of the Sphingolipidome of the Peri-Infarct Tissue during Hemorrhagic Transformation in a Mouse Model of Cerebral Ischemia |
| title_full | Characterization of the Sphingolipidome of the Peri-Infarct Tissue during Hemorrhagic Transformation in a Mouse Model of Cerebral Ischemia |
| title_fullStr | Characterization of the Sphingolipidome of the Peri-Infarct Tissue during Hemorrhagic Transformation in a Mouse Model of Cerebral Ischemia |
| title_full_unstemmed | Characterization of the Sphingolipidome of the Peri-Infarct Tissue during Hemorrhagic Transformation in a Mouse Model of Cerebral Ischemia |
| title_short | Characterization of the Sphingolipidome of the Peri-Infarct Tissue during Hemorrhagic Transformation in a Mouse Model of Cerebral Ischemia |
| title_sort | characterization of the sphingolipidome of the peri infarct tissue during hemorrhagic transformation in a mouse model of cerebral ischemia |
| topic | sphingosine 1-phosphate ceramide s1p 1 ischemic stroke hemorrhagic transformation sphingolipid profiling mcao c57bl/6 |
| url | https://www.imrpress.com/journal/JIN/21/6/10.31083/j.jin2106161 |
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