Method comparison for Japanese encephalitis virus detection in samples collected from the Indo-Pacific region
IntroductionJapanese encephalitis virus (JEV) is a mosquito-borne viral pathogen, which is becoming a growing public health concern throughout the Indo-Pacific. Five genotypes of JEV have been identified. Current vaccines are based on genotype III and provide a high degree of protection for four of...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Public Health |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpubh.2022.1051754/full |
| _version_ | 1811179985072816128 |
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| author | Gary Crispell Kelly Williams Eric Zielinski Akira Iwami Zachary Homas Karen Thomas |
| author_facet | Gary Crispell Kelly Williams Eric Zielinski Akira Iwami Zachary Homas Karen Thomas |
| author_sort | Gary Crispell |
| collection | DOAJ |
| description | IntroductionJapanese encephalitis virus (JEV) is a mosquito-borne viral pathogen, which is becoming a growing public health concern throughout the Indo-Pacific. Five genotypes of JEV have been identified. Current vaccines are based on genotype III and provide a high degree of protection for four of the five known genotypes.MethodsRT-PCR, Magpix, Twist Biosciences Comprehensive Viral Research Panel (CVRP), and SISPA methods were used to detect JEV from mosquito samples collected in South Korea during 2021. These methods were compared to determine which method would be most effective for biosurveillance in the Indo-Pacific region.ResultsOur data showed that RT-PCR, Twist CVRP, and SISPA methods were all able to detect JEV genotype I, however, the proprietary Magpix panel was only able to detect JEV genotype III. Use of minION sequencing for pathogen detection in arthropod samples will require further method development.ConclusionBiosurveillance of vectorborne pathogens remains an area of concern throughout the Indo-Pacific. RT-PCR was the most cost effective method used in the study, but TWIST CVRP allows for the identification of over 3,100 viral genomes. Further research and comparisons will be conducted to ensure optimal methods are used for large scale biosurveillance. |
| first_indexed | 2024-04-11T06:42:33Z |
| format | Article |
| id | doaj.art-753a9b9d97374cfdb71433c6b347b337 |
| institution | Directory Open Access Journal |
| issn | 2296-2565 |
| language | English |
| last_indexed | 2024-04-11T06:42:33Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Public Health |
| spelling | doaj.art-753a9b9d97374cfdb71433c6b347b3372022-12-22T04:39:28ZengFrontiers Media S.A.Frontiers in Public Health2296-25652022-11-011010.3389/fpubh.2022.10517541051754Method comparison for Japanese encephalitis virus detection in samples collected from the Indo-Pacific regionGary CrispellKelly WilliamsEric ZielinskiAkira IwamiZachary HomasKaren ThomasIntroductionJapanese encephalitis virus (JEV) is a mosquito-borne viral pathogen, which is becoming a growing public health concern throughout the Indo-Pacific. Five genotypes of JEV have been identified. Current vaccines are based on genotype III and provide a high degree of protection for four of the five known genotypes.MethodsRT-PCR, Magpix, Twist Biosciences Comprehensive Viral Research Panel (CVRP), and SISPA methods were used to detect JEV from mosquito samples collected in South Korea during 2021. These methods were compared to determine which method would be most effective for biosurveillance in the Indo-Pacific region.ResultsOur data showed that RT-PCR, Twist CVRP, and SISPA methods were all able to detect JEV genotype I, however, the proprietary Magpix panel was only able to detect JEV genotype III. Use of minION sequencing for pathogen detection in arthropod samples will require further method development.ConclusionBiosurveillance of vectorborne pathogens remains an area of concern throughout the Indo-Pacific. RT-PCR was the most cost effective method used in the study, but TWIST CVRP allows for the identification of over 3,100 viral genomes. Further research and comparisons will be conducted to ensure optimal methods are used for large scale biosurveillance.https://www.frontiersin.org/articles/10.3389/fpubh.2022.1051754/fullJapanese encephalitis virus (JEV)MagpixSISPAminION deviceMiSeqTwist Comprehensive Viral Research Panel (CVRP) |
| spellingShingle | Gary Crispell Kelly Williams Eric Zielinski Akira Iwami Zachary Homas Karen Thomas Method comparison for Japanese encephalitis virus detection in samples collected from the Indo-Pacific region Frontiers in Public Health Japanese encephalitis virus (JEV) Magpix SISPA minION device MiSeq Twist Comprehensive Viral Research Panel (CVRP) |
| title | Method comparison for Japanese encephalitis virus detection in samples collected from the Indo-Pacific region |
| title_full | Method comparison for Japanese encephalitis virus detection in samples collected from the Indo-Pacific region |
| title_fullStr | Method comparison for Japanese encephalitis virus detection in samples collected from the Indo-Pacific region |
| title_full_unstemmed | Method comparison for Japanese encephalitis virus detection in samples collected from the Indo-Pacific region |
| title_short | Method comparison for Japanese encephalitis virus detection in samples collected from the Indo-Pacific region |
| title_sort | method comparison for japanese encephalitis virus detection in samples collected from the indo pacific region |
| topic | Japanese encephalitis virus (JEV) Magpix SISPA minION device MiSeq Twist Comprehensive Viral Research Panel (CVRP) |
| url | https://www.frontiersin.org/articles/10.3389/fpubh.2022.1051754/full |
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