Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet
BACKGROUND: Metabolic syndrome is a growing worldwide health problem. We evaluated the effects of wine grape powder (WGP), rich in antioxidants and fiber, in a rat model of metabolic syndrome induced by a high fructose diet. We tested whether WGP supplementation may prevent glucose intolerance and d...
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| Format: | Article |
| Language: | English |
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BMC
2015-01-01
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| Series: | Biological Research |
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| Online Access: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100053&lng=en&tlng=en |
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| author | Romina Hernández-Salinas Valerie Decap Alberto Leguina Patricio Cáceres Druso Perez Ines Urquiaga Rodrigo Iturriaga Victoria Velarde |
| author_facet | Romina Hernández-Salinas Valerie Decap Alberto Leguina Patricio Cáceres Druso Perez Ines Urquiaga Rodrigo Iturriaga Victoria Velarde |
| author_sort | Romina Hernández-Salinas |
| collection | DOAJ |
| description | BACKGROUND: Metabolic syndrome is a growing worldwide health problem. We evaluated the effects of wine grape powder (WGP), rich in antioxidants and fiber, in a rat model of metabolic syndrome induced by a high fructose diet. We tested whether WGP supplementation may prevent glucose intolerance and decrease oxidative stress in rats fed with a high fructose diet. METHODS: Male Sprague-Dawley rats weighing 180 g were divided into four groups according to their feeding protocols. Rats were fed with control diet (C), control plus 20 % WGP (C + WGP), 50 % high fructose (HF) or 50 % fructose plus 20 % WGP (HF + WGP) for 16 weeks. Blood glucose, insulin and triglycerides, weight, and arterial blood pressure were measured. Homeostasis model assessment (HOMA) index was calculated using insulin and glucose values. A glucose tolerance test was performed 2 days before the end of the experiment. As an index of oxidative stress, thio-barbituric acid reactive substances (TBARS) level was measured in plasma and kidney, and superoxide dismutase was measured in the kidney. RESULTS: Thiobarbituric acid reactive substances in plasma and renal tissue were significantly higher when compared to the control group. In addition, the area under the curve of the glucose tolerance test was higher in HF fed animals. Furthermore, fasting blood glucose, plasma insulin levels, and the HOMA index, were also increased. WGP supplementation prevented these alterations in rats fed with the HF diet. We did not find any significant difference in body weight or systolic blood pressure in any of the groups. CONCLUSIONS: Our results show that WGP supplementation prevented hyperglycemia, insulin resistance and reduced oxidative stress in rats fed with HF diet. We propose that WGP may be used as a supplement in human food as well. |
| first_indexed | 2024-12-13T19:04:57Z |
| format | Article |
| id | doaj.art-753aeb0761774753a968409586d069a0 |
| institution | Directory Open Access Journal |
| issn | 0716-9760 |
| language | English |
| last_indexed | 2024-12-13T19:04:57Z |
| publishDate | 2015-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Research |
| spelling | doaj.art-753aeb0761774753a968409586d069a02022-12-21T23:34:35ZengBMCBiological Research0716-97602015-01-014801910.1186/s40659-015-0045-4S0716-97602015000100053Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose dietRomina Hernández-Salinas0Valerie Decap1Alberto Leguina2Patricio Cáceres3Druso Perez4Ines Urquiaga5Rodrigo Iturriaga6Victoria Velarde7Pontifícia Universidad Católica de ChilePontifícia Universidad Católica de ChilePontifícia Universidad Católica de ChilePontifícia Universidad Católica de ChilePontifícia Universidad Católica de ChilePontifícia Universidad Católica de ChilePontifícia Universidad Católica de ChilePontifícia Universidad Católica de ChileBACKGROUND: Metabolic syndrome is a growing worldwide health problem. We evaluated the effects of wine grape powder (WGP), rich in antioxidants and fiber, in a rat model of metabolic syndrome induced by a high fructose diet. We tested whether WGP supplementation may prevent glucose intolerance and decrease oxidative stress in rats fed with a high fructose diet. METHODS: Male Sprague-Dawley rats weighing 180 g were divided into four groups according to their feeding protocols. Rats were fed with control diet (C), control plus 20 % WGP (C + WGP), 50 % high fructose (HF) or 50 % fructose plus 20 % WGP (HF + WGP) for 16 weeks. Blood glucose, insulin and triglycerides, weight, and arterial blood pressure were measured. Homeostasis model assessment (HOMA) index was calculated using insulin and glucose values. A glucose tolerance test was performed 2 days before the end of the experiment. As an index of oxidative stress, thio-barbituric acid reactive substances (TBARS) level was measured in plasma and kidney, and superoxide dismutase was measured in the kidney. RESULTS: Thiobarbituric acid reactive substances in plasma and renal tissue were significantly higher when compared to the control group. In addition, the area under the curve of the glucose tolerance test was higher in HF fed animals. Furthermore, fasting blood glucose, plasma insulin levels, and the HOMA index, were also increased. WGP supplementation prevented these alterations in rats fed with the HF diet. We did not find any significant difference in body weight or systolic blood pressure in any of the groups. CONCLUSIONS: Our results show that WGP supplementation prevented hyperglycemia, insulin resistance and reduced oxidative stress in rats fed with HF diet. We propose that WGP may be used as a supplement in human food as well.http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100053&lng=en&tlng=enInsulin resistanceOxidative stressMetabolic syndromeWine grape powder |
| spellingShingle | Romina Hernández-Salinas Valerie Decap Alberto Leguina Patricio Cáceres Druso Perez Ines Urquiaga Rodrigo Iturriaga Victoria Velarde Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet Biological Research Insulin resistance Oxidative stress Metabolic syndrome Wine grape powder |
| title | Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet |
| title_full | Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet |
| title_fullStr | Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet |
| title_full_unstemmed | Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet |
| title_short | Antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet |
| title_sort | antioxidant and anti hyperglycemic role of wine grape powder in rats fed with a high fructose diet |
| topic | Insulin resistance Oxidative stress Metabolic syndrome Wine grape powder |
| url | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602015000100053&lng=en&tlng=en |
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