Phenotypic and functional profile of IFN-α-differentiated dendritic cells (IFN-DCs) from HIV-infected individuals
Dendritic cell (DC)-based immunotherapy is a promising strategy for the treatment of HIV-infected individuals. Different from the conventional protocol for DC differentiation based on the cytokine IL-4 (IL4-DCs), several studies have suggested obtaining DCs by culturing monocytes with type I IFN (IF...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-09-01
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| Series: | Human Vaccines & Immunotherapeutics |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21645515.2018.1547603 |
| _version_ | 1797677414863601664 |
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| author | Bruna Tereso Santillo Denise da Silva Reis Laís Teodoro da Silva Nathalia Teixeira Romani Alberto José da Silva Duarte Telma Miyuki Oshiro |
| author_facet | Bruna Tereso Santillo Denise da Silva Reis Laís Teodoro da Silva Nathalia Teixeira Romani Alberto José da Silva Duarte Telma Miyuki Oshiro |
| author_sort | Bruna Tereso Santillo |
| collection | DOAJ |
| description | Dendritic cell (DC)-based immunotherapy is a promising strategy for the treatment of HIV-infected individuals. Different from the conventional protocol for DC differentiation based on the cytokine IL-4 (IL4-DCs), several studies have suggested obtaining DCs by culturing monocytes with type I IFN (IFN-α) to yield IFN-DCs, as performed in cancer therapy. To evaluate the phenotypic and functional characteristics, monocytes from HIV-infected subjects were differentiated into IFN-DCs or IL4-DCs, pulsed with chemically inactivated HIV and stimulated with pro-inflammatory cytokines. A comparative analysis between both types of monocyte-derived DCs (MoDCs) showed that immature IFN-DCs were phenotypically distinct from immature IL4-DCs at the baseline of differentiation, presenting a pre-activated profile. From the functional profile, we determined that IFN-DCs were capable of producing the cytokine IL-12 p70 and of inducing the production of IFN-γ by CD4 + T lymphocytes but not by TCD8+ lymphocytes. Our results suggest that IFN-DCs derived from HIV-infected individuals are able to recognize and present viral antigens to induce TCD4+ cellular immunity to HIV. |
| first_indexed | 2024-03-11T22:44:54Z |
| format | Article |
| id | doaj.art-753d9c0389334cd2a66de110b3f910bd |
| institution | Directory Open Access Journal |
| issn | 2164-5515 2164-554X |
| language | English |
| last_indexed | 2024-03-11T22:44:54Z |
| publishDate | 2019-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Human Vaccines & Immunotherapeutics |
| spelling | doaj.art-753d9c0389334cd2a66de110b3f910bd2023-09-22T08:38:24ZengTaylor & Francis GroupHuman Vaccines & Immunotherapeutics2164-55152164-554X2019-09-011592140214910.1080/21645515.2018.15476031547603Phenotypic and functional profile of IFN-α-differentiated dendritic cells (IFN-DCs) from HIV-infected individualsBruna Tereso Santillo0Denise da Silva Reis1Laís Teodoro da Silva2Nathalia Teixeira Romani3Alberto José da Silva Duarte4Telma Miyuki Oshiro5Universidade de Sao PauloUniversidade de Sao PauloUniversidade de Sao PauloUniversidade de Sao PauloUniversidade de Sao PauloUniversidade de Sao PauloDendritic cell (DC)-based immunotherapy is a promising strategy for the treatment of HIV-infected individuals. Different from the conventional protocol for DC differentiation based on the cytokine IL-4 (IL4-DCs), several studies have suggested obtaining DCs by culturing monocytes with type I IFN (IFN-α) to yield IFN-DCs, as performed in cancer therapy. To evaluate the phenotypic and functional characteristics, monocytes from HIV-infected subjects were differentiated into IFN-DCs or IL4-DCs, pulsed with chemically inactivated HIV and stimulated with pro-inflammatory cytokines. A comparative analysis between both types of monocyte-derived DCs (MoDCs) showed that immature IFN-DCs were phenotypically distinct from immature IL4-DCs at the baseline of differentiation, presenting a pre-activated profile. From the functional profile, we determined that IFN-DCs were capable of producing the cytokine IL-12 p70 and of inducing the production of IFN-γ by CD4 + T lymphocytes but not by TCD8+ lymphocytes. Our results suggest that IFN-DCs derived from HIV-infected individuals are able to recognize and present viral antigens to induce TCD4+ cellular immunity to HIV.http://dx.doi.org/10.1080/21645515.2018.1547603dendritic cellstype i interferonhivimmunotherapy |
| spellingShingle | Bruna Tereso Santillo Denise da Silva Reis Laís Teodoro da Silva Nathalia Teixeira Romani Alberto José da Silva Duarte Telma Miyuki Oshiro Phenotypic and functional profile of IFN-α-differentiated dendritic cells (IFN-DCs) from HIV-infected individuals Human Vaccines & Immunotherapeutics dendritic cells type i interferon hiv immunotherapy |
| title | Phenotypic and functional profile of IFN-α-differentiated dendritic cells (IFN-DCs) from HIV-infected individuals |
| title_full | Phenotypic and functional profile of IFN-α-differentiated dendritic cells (IFN-DCs) from HIV-infected individuals |
| title_fullStr | Phenotypic and functional profile of IFN-α-differentiated dendritic cells (IFN-DCs) from HIV-infected individuals |
| title_full_unstemmed | Phenotypic and functional profile of IFN-α-differentiated dendritic cells (IFN-DCs) from HIV-infected individuals |
| title_short | Phenotypic and functional profile of IFN-α-differentiated dendritic cells (IFN-DCs) from HIV-infected individuals |
| title_sort | phenotypic and functional profile of ifn α differentiated dendritic cells ifn dcs from hiv infected individuals |
| topic | dendritic cells type i interferon hiv immunotherapy |
| url | http://dx.doi.org/10.1080/21645515.2018.1547603 |
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