Bicarbonate Increases Ischemia-Reperfusion Damage by Inhibiting Mitophagy.
During an ischemic event, bicarbonate and CO2 concentration increase as a consequence of O2 consumption and lack of blood flow. This event is important as bicarbonate/CO2 is determinant for several redox and enzymatic reactions, in addition to pH regulation. Until now, most work done on the role of...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5156406?pdf=render |
| _version_ | 1818947837926309888 |
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| author | Bruno B Queliconi Alicia J Kowaltowski Roberta A Gottlieb |
| author_facet | Bruno B Queliconi Alicia J Kowaltowski Roberta A Gottlieb |
| author_sort | Bruno B Queliconi |
| collection | DOAJ |
| description | During an ischemic event, bicarbonate and CO2 concentration increase as a consequence of O2 consumption and lack of blood flow. This event is important as bicarbonate/CO2 is determinant for several redox and enzymatic reactions, in addition to pH regulation. Until now, most work done on the role of bicarbonate in ischemia-reperfusion injury focused on pH changes; although reperfusion solutions have a fixed pH, cardiac resuscitation protocols commonly employ bicarbonate to correct the profound acidosis associated with respiratory arrest. However, we previously showed that bicarbonate can increase tissue damage and protein oxidative damage independent of pH. Here we show the molecular basis of bicarbonate-induced reperfusion damage: the presence of bicarbonate selectively impairs mitophagy, with no detectable effect on autophagy, proteasome activity, reactive oxygen species production or protein oxidation. We also show that inhibition of autophagy reproduces the effects of bicarbonate in reperfusion injury, providing additional evidence in support of this mechanism. This phenomenon is especially important because bicarbonate is widely used in resuscitation protocols after cardiac arrest, and while effective as a buffer, may also contribute to myocardial injury. |
| first_indexed | 2024-12-20T08:37:15Z |
| format | Article |
| id | doaj.art-75408c5993914bed9343f44c948c0303 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T08:37:15Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-75408c5993914bed9343f44c948c03032022-12-21T19:46:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-011112e016767810.1371/journal.pone.0167678Bicarbonate Increases Ischemia-Reperfusion Damage by Inhibiting Mitophagy.Bruno B QueliconiAlicia J KowaltowskiRoberta A GottliebDuring an ischemic event, bicarbonate and CO2 concentration increase as a consequence of O2 consumption and lack of blood flow. This event is important as bicarbonate/CO2 is determinant for several redox and enzymatic reactions, in addition to pH regulation. Until now, most work done on the role of bicarbonate in ischemia-reperfusion injury focused on pH changes; although reperfusion solutions have a fixed pH, cardiac resuscitation protocols commonly employ bicarbonate to correct the profound acidosis associated with respiratory arrest. However, we previously showed that bicarbonate can increase tissue damage and protein oxidative damage independent of pH. Here we show the molecular basis of bicarbonate-induced reperfusion damage: the presence of bicarbonate selectively impairs mitophagy, with no detectable effect on autophagy, proteasome activity, reactive oxygen species production or protein oxidation. We also show that inhibition of autophagy reproduces the effects of bicarbonate in reperfusion injury, providing additional evidence in support of this mechanism. This phenomenon is especially important because bicarbonate is widely used in resuscitation protocols after cardiac arrest, and while effective as a buffer, may also contribute to myocardial injury.http://europepmc.org/articles/PMC5156406?pdf=render |
| spellingShingle | Bruno B Queliconi Alicia J Kowaltowski Roberta A Gottlieb Bicarbonate Increases Ischemia-Reperfusion Damage by Inhibiting Mitophagy. PLoS ONE |
| title | Bicarbonate Increases Ischemia-Reperfusion Damage by Inhibiting Mitophagy. |
| title_full | Bicarbonate Increases Ischemia-Reperfusion Damage by Inhibiting Mitophagy. |
| title_fullStr | Bicarbonate Increases Ischemia-Reperfusion Damage by Inhibiting Mitophagy. |
| title_full_unstemmed | Bicarbonate Increases Ischemia-Reperfusion Damage by Inhibiting Mitophagy. |
| title_short | Bicarbonate Increases Ischemia-Reperfusion Damage by Inhibiting Mitophagy. |
| title_sort | bicarbonate increases ischemia reperfusion damage by inhibiting mitophagy |
| url | http://europepmc.org/articles/PMC5156406?pdf=render |
| work_keys_str_mv | AT brunobqueliconi bicarbonateincreasesischemiareperfusiondamagebyinhibitingmitophagy AT aliciajkowaltowski bicarbonateincreasesischemiareperfusiondamagebyinhibitingmitophagy AT robertaagottlieb bicarbonateincreasesischemiareperfusiondamagebyinhibitingmitophagy |