A gradient of Wnt activity positions the neurosensory domains of the inner ear
The auditory and vestibular organs of the inner ear and the neurons that innervate them originate from Sox2-positive and Notch-active neurosensory domains specified at early stages of otic development. Sox2 is initially present throughout the otic placode and otocyst, and then it becomes progressive...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2021-03-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/59540 |
| _version_ | 1797997562844676096 |
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| author | Magdalena Żak Nicolas Daudet |
| author_facet | Magdalena Żak Nicolas Daudet |
| author_sort | Magdalena Żak |
| collection | DOAJ |
| description | The auditory and vestibular organs of the inner ear and the neurons that innervate them originate from Sox2-positive and Notch-active neurosensory domains specified at early stages of otic development. Sox2 is initially present throughout the otic placode and otocyst, and then it becomes progressively restricted to a ventro-medial domain. Using gain- and loss-of-function approaches in the chicken otocyst, we show that these early changes in Sox2 expression are regulated in a dose-dependent manner by Wnt/beta-catenin signalling. Both high and very low levels of Wnt activity repress Sox2 and neurosensory competence. However, intermediate levels allow the maintenance of Sox2 expression and sensory organ formation. We propose that a dorso-ventral (high-to-low) gradient and wave of Wnt activity initiated at the dorsal rim of the otic placode progressively restricts Sox2 and Notch activity to the ventral half of the otocyst, thereby positioning the neurosensory competent domains in the inner ear. |
| first_indexed | 2024-04-11T10:34:38Z |
| format | Article |
| id | doaj.art-7544900e1d3744d6971373039f7b16cf |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T10:34:38Z |
| publishDate | 2021-03-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-7544900e1d3744d6971373039f7b16cf2022-12-22T04:29:20ZengeLife Sciences Publications LtdeLife2050-084X2021-03-011010.7554/eLife.59540A gradient of Wnt activity positions the neurosensory domains of the inner earMagdalena Żak0Nicolas Daudet1https://orcid.org/0000-0002-4039-4716UCL Ear Institute, University College London, London, United KingdomUCL Ear Institute, University College London, London, United KingdomThe auditory and vestibular organs of the inner ear and the neurons that innervate them originate from Sox2-positive and Notch-active neurosensory domains specified at early stages of otic development. Sox2 is initially present throughout the otic placode and otocyst, and then it becomes progressively restricted to a ventro-medial domain. Using gain- and loss-of-function approaches in the chicken otocyst, we show that these early changes in Sox2 expression are regulated in a dose-dependent manner by Wnt/beta-catenin signalling. Both high and very low levels of Wnt activity repress Sox2 and neurosensory competence. However, intermediate levels allow the maintenance of Sox2 expression and sensory organ formation. We propose that a dorso-ventral (high-to-low) gradient and wave of Wnt activity initiated at the dorsal rim of the otic placode progressively restricts Sox2 and Notch activity to the ventral half of the otocyst, thereby positioning the neurosensory competent domains in the inner ear.https://elifesciences.org/articles/59540inner earWnt signallingsensory organtissue patterning |
| spellingShingle | Magdalena Żak Nicolas Daudet A gradient of Wnt activity positions the neurosensory domains of the inner ear eLife inner ear Wnt signalling sensory organ tissue patterning |
| title | A gradient of Wnt activity positions the neurosensory domains of the inner ear |
| title_full | A gradient of Wnt activity positions the neurosensory domains of the inner ear |
| title_fullStr | A gradient of Wnt activity positions the neurosensory domains of the inner ear |
| title_full_unstemmed | A gradient of Wnt activity positions the neurosensory domains of the inner ear |
| title_short | A gradient of Wnt activity positions the neurosensory domains of the inner ear |
| title_sort | gradient of wnt activity positions the neurosensory domains of the inner ear |
| topic | inner ear Wnt signalling sensory organ tissue patterning |
| url | https://elifesciences.org/articles/59540 |
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