The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe.
A strand-specific transcriptome sequencing strategy, directional ligation sequencing or DeLi-seq, was employed to profile antisense transcriptome of Schizosaccharomyces pombe. Under both normal and heat shock conditions, we found that polyadenylated antisense transcripts are broadly expressed while...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2010-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3004915?pdf=render |
| _version_ | 1818021111155130368 |
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| author | Ting Ni Kang Tu Zhong Wang Shen Song Han Wu Bin Xie Kristin C Scott Shiv I Grewal Yuan Gao Jun Zhu |
| author_facet | Ting Ni Kang Tu Zhong Wang Shen Song Han Wu Bin Xie Kristin C Scott Shiv I Grewal Yuan Gao Jun Zhu |
| author_sort | Ting Ni |
| collection | DOAJ |
| description | A strand-specific transcriptome sequencing strategy, directional ligation sequencing or DeLi-seq, was employed to profile antisense transcriptome of Schizosaccharomyces pombe. Under both normal and heat shock conditions, we found that polyadenylated antisense transcripts are broadly expressed while distinct expression patterns were observed for protein-coding and non-coding loci. Dominant antisense expression is enriched in protein-coding genes involved in meiosis or stress response pathways. Detailed analyses further suggest that antisense transcripts are independently regulated with respect to their sense transcripts, and diverse mechanisms might be potentially involved in the biogenesis and degradation of antisense RNAs. Taken together, antisense transcription may have profound impacts on global gene regulation in S. pombe. |
| first_indexed | 2024-04-14T08:14:24Z |
| format | Article |
| id | doaj.art-75492228d0874e5b8fb54cfc90571914 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-14T08:14:24Z |
| publishDate | 2010-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-75492228d0874e5b8fb54cfc905719142022-12-22T02:04:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032010-01-01512e1527110.1371/journal.pone.0015271The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe.Ting NiKang TuZhong WangShen SongHan WuBin XieKristin C ScottShiv I GrewalYuan GaoJun ZhuA strand-specific transcriptome sequencing strategy, directional ligation sequencing or DeLi-seq, was employed to profile antisense transcriptome of Schizosaccharomyces pombe. Under both normal and heat shock conditions, we found that polyadenylated antisense transcripts are broadly expressed while distinct expression patterns were observed for protein-coding and non-coding loci. Dominant antisense expression is enriched in protein-coding genes involved in meiosis or stress response pathways. Detailed analyses further suggest that antisense transcripts are independently regulated with respect to their sense transcripts, and diverse mechanisms might be potentially involved in the biogenesis and degradation of antisense RNAs. Taken together, antisense transcription may have profound impacts on global gene regulation in S. pombe.http://europepmc.org/articles/PMC3004915?pdf=render |
| spellingShingle | Ting Ni Kang Tu Zhong Wang Shen Song Han Wu Bin Xie Kristin C Scott Shiv I Grewal Yuan Gao Jun Zhu The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe. PLoS ONE |
| title | The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe. |
| title_full | The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe. |
| title_fullStr | The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe. |
| title_full_unstemmed | The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe. |
| title_short | The prevalence and regulation of antisense transcripts in Schizosaccharomyces pombe. |
| title_sort | prevalence and regulation of antisense transcripts in schizosaccharomyces pombe |
| url | http://europepmc.org/articles/PMC3004915?pdf=render |
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