Long-term in vitro 2D-culture of SDHB and SDHD-related human paragangliomas and pheochromocytomas
The neuroendocrine tumours paraganglioma and pheochromocytoma (PPGLs) are commonly associated with succinate dehydrogenase (SDH) gene variants, but no human SDH-related PPGL-derived cell line has been developed to date. The aim of this study was to systematically explore practical issues related to...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9524698/?tool=EBI |
| _version_ | 1811181740155207680 |
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| author | Jean-Pierre Bayley Heggert G. Rebel Kimberly Scheurwater Dominique Duesman Juan Zhang Francesca Schiavi Esther Korpershoek Jeroen C. Jansen Abbey Schepers Peter Devilee |
| author_facet | Jean-Pierre Bayley Heggert G. Rebel Kimberly Scheurwater Dominique Duesman Juan Zhang Francesca Schiavi Esther Korpershoek Jeroen C. Jansen Abbey Schepers Peter Devilee |
| author_sort | Jean-Pierre Bayley |
| collection | DOAJ |
| description | The neuroendocrine tumours paraganglioma and pheochromocytoma (PPGLs) are commonly associated with succinate dehydrogenase (SDH) gene variants, but no human SDH-related PPGL-derived cell line has been developed to date. The aim of this study was to systematically explore practical issues related to the classical 2D-culture of SDH-related human paragangliomas and pheochromocytomas, with the ultimate goal of identifying a viable tumour-derived cell line. PPGL tumour tissue/cells (chromaffin cells) were cultured in a variety of media formulations and supplements. Tumour explants and dissociated primary tumour cells were cultured and stained with a range of antibodies to identify markers suitable for use in human PPGL culture. We cultured 62 PPGLs, including tumours with confirmed SDHB, SDHC and SDHD variants, as well as several metastatic tumours. Testing a wide range of basic cell culture media and supplements, we noted a marked decline in chromaffin cell numbers over a 4–8 week period but the persistence of small numbers of synaptophysin/tyrosine hydroxylase-positive chromaffin cells for up to 99 weeks. In cell culture, immunohistochemical staining for chromogranin A and neuron-specific enolase was generally negative in chromaffin cells, while staining for synaptophysin and tyrosine hydroxylase was generally positive. GFAP showed the most consistent staining of type II sustentacular cells. Of the media tested, low serum or serum-free media best sustained relative chromaffin cell numbers, while lactate enhanced the survival of synaptophysin-positive cells. Synaptophysin-positive PPGL tumour cells persist in culture for long periods but show little evidence of proliferation. Synaptophysin was the most consistent cell marker for chromaffin cells and GFAP the best marker for sustentacular cells in human PPGL cultures. |
| first_indexed | 2024-04-11T09:22:37Z |
| format | Article |
| id | doaj.art-754bbd15a887414e97912e1990d8679d |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T09:22:37Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-754bbd15a887414e97912e1990d8679d2022-12-22T04:32:09ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01179Long-term in vitro 2D-culture of SDHB and SDHD-related human paragangliomas and pheochromocytomasJean-Pierre BayleyHeggert G. RebelKimberly ScheurwaterDominique DuesmanJuan ZhangFrancesca SchiaviEsther KorpershoekJeroen C. JansenAbbey SchepersPeter DevileeThe neuroendocrine tumours paraganglioma and pheochromocytoma (PPGLs) are commonly associated with succinate dehydrogenase (SDH) gene variants, but no human SDH-related PPGL-derived cell line has been developed to date. The aim of this study was to systematically explore practical issues related to the classical 2D-culture of SDH-related human paragangliomas and pheochromocytomas, with the ultimate goal of identifying a viable tumour-derived cell line. PPGL tumour tissue/cells (chromaffin cells) were cultured in a variety of media formulations and supplements. Tumour explants and dissociated primary tumour cells were cultured and stained with a range of antibodies to identify markers suitable for use in human PPGL culture. We cultured 62 PPGLs, including tumours with confirmed SDHB, SDHC and SDHD variants, as well as several metastatic tumours. Testing a wide range of basic cell culture media and supplements, we noted a marked decline in chromaffin cell numbers over a 4–8 week period but the persistence of small numbers of synaptophysin/tyrosine hydroxylase-positive chromaffin cells for up to 99 weeks. In cell culture, immunohistochemical staining for chromogranin A and neuron-specific enolase was generally negative in chromaffin cells, while staining for synaptophysin and tyrosine hydroxylase was generally positive. GFAP showed the most consistent staining of type II sustentacular cells. Of the media tested, low serum or serum-free media best sustained relative chromaffin cell numbers, while lactate enhanced the survival of synaptophysin-positive cells. Synaptophysin-positive PPGL tumour cells persist in culture for long periods but show little evidence of proliferation. Synaptophysin was the most consistent cell marker for chromaffin cells and GFAP the best marker for sustentacular cells in human PPGL cultures.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9524698/?tool=EBI |
| spellingShingle | Jean-Pierre Bayley Heggert G. Rebel Kimberly Scheurwater Dominique Duesman Juan Zhang Francesca Schiavi Esther Korpershoek Jeroen C. Jansen Abbey Schepers Peter Devilee Long-term in vitro 2D-culture of SDHB and SDHD-related human paragangliomas and pheochromocytomas PLoS ONE |
| title | Long-term in vitro 2D-culture of SDHB and SDHD-related human paragangliomas and pheochromocytomas |
| title_full | Long-term in vitro 2D-culture of SDHB and SDHD-related human paragangliomas and pheochromocytomas |
| title_fullStr | Long-term in vitro 2D-culture of SDHB and SDHD-related human paragangliomas and pheochromocytomas |
| title_full_unstemmed | Long-term in vitro 2D-culture of SDHB and SDHD-related human paragangliomas and pheochromocytomas |
| title_short | Long-term in vitro 2D-culture of SDHB and SDHD-related human paragangliomas and pheochromocytomas |
| title_sort | long term in vitro 2d culture of sdhb and sdhd related human paragangliomas and pheochromocytomas |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9524698/?tool=EBI |
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