Pseudomonas aeruginosa Y12 play positive roles regulating larval gut communities when housefly encountered copper stress
Heavy metal contamination has become a global concern that threatens the lives of animals and insects throughout the food chain. Pseudomonas is a commonly found genus of bacteria that colonizes the intestines of insects and constitutes a necessary part of the insect gut microbiota. This research ana...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-06-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323004827 |
| _version_ | 1827943939524526080 |
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| author | Shumin Wang Kexin Zhang Qian Zhang Ying Li Yansong Yin Wenjuan Liu Sha An Ruiling Zhang Zhong Zhang |
| author_facet | Shumin Wang Kexin Zhang Qian Zhang Ying Li Yansong Yin Wenjuan Liu Sha An Ruiling Zhang Zhong Zhang |
| author_sort | Shumin Wang |
| collection | DOAJ |
| description | Heavy metal contamination has become a global concern that threatens the lives of animals and insects throughout the food chain. Pseudomonas is a commonly found genus of bacteria that colonizes the intestines of insects and constitutes a necessary part of the insect gut microbiota. This research analyzed the influence of different concentrations of Cu2+ on housefly larval development, gut microbial structure and antioxidant defense system, and investigated the regulatory mechanism of P. aeruginosa Y12 on the gut microbiota when houseflies were exposed to Cu2+. We found that adding Cu2+ to the larval diet inhibited larval growth, while the mixed addition of P. aeruginosa Y12 and Cu2+ to the diet reduced the inhibitory effects of Cu2+ on larval growth. Oral administration of Cu2+ significantly changed the gut community structure and increased larval gut bacterial diversity. In vitro analysis revealed that P. aeruginosa Y12 showed Cu2+ adsorption effects and increased Cu2+ aggregation. The mixed addition of low concentrations of P. aeruginosa Y12 and Cu2+ to the larval diet caused a dynamic shift in the gut microbiota and resulted in a novel gut community structure with an increase in beneficial bacteria and a decrease in pathogenic bacteria. Furthermore, P. aeruginosa Y12 treatment influenced the activity of antioxidant enzymes in housefly larvae, indicating that the addition of P. aeruginosa Y12 to the larval diet could further influence the antioxidant system through P. aeruginosa Y12-Cu2+ interactions. In conclusion, our research revealed that intestinal flora dysbiosis was the essential reason why copper inhibits housefly larval growth. However, proper supplementation with P. aeruginosa Y12 played positive roles in regulating larval gut communities and protecting insects from toxic heavy metals. |
| first_indexed | 2024-03-13T10:22:54Z |
| format | Article |
| id | doaj.art-754e230c913345eda39c413461000fcb |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-03-13T10:22:54Z |
| publishDate | 2023-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-754e230c913345eda39c413461000fcb2023-05-20T04:29:05ZengElsevierEcotoxicology and Environmental Safety0147-65132023-06-01258114978Pseudomonas aeruginosa Y12 play positive roles regulating larval gut communities when housefly encountered copper stressShumin Wang0Kexin Zhang1Qian Zhang2Ying Li3Yansong Yin4Wenjuan Liu5Sha An6Ruiling Zhang7Zhong Zhang8School of Life Science, Shandong First Medical University (Shandong Academy of Medical Sciences), Taian 271016, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University (Shandong Academy of Medical Sciences), Taian 271016, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University (Shandong Academy of Medical Sciences), Taian 271016, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University (Shandong Academy of Medical Sciences), Taian 271016, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University (Shandong Academy of Medical Sciences), Taian 271016, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University (Shandong Academy of Medical Sciences), Taian 271016, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University (Shandong Academy of Medical Sciences), Taian 271016, Shandong, ChinaSchool of Basic Medical Science, Shandong First Medical University (Shandong Academy of Medical Sciences), Taian 271016, Shandong, China; Corresponding author.School of Life Science, Shandong First Medical University (Shandong Academy of Medical Sciences), Taian 271016, Shandong, China; Weifang Medical University, Weifang 261021, Shandong, China; Corresponding author at: School of Life Science, Shandong First Medical University (Shandong Academy of Medical Sciences), Taian 271016, Shandong, China.Heavy metal contamination has become a global concern that threatens the lives of animals and insects throughout the food chain. Pseudomonas is a commonly found genus of bacteria that colonizes the intestines of insects and constitutes a necessary part of the insect gut microbiota. This research analyzed the influence of different concentrations of Cu2+ on housefly larval development, gut microbial structure and antioxidant defense system, and investigated the regulatory mechanism of P. aeruginosa Y12 on the gut microbiota when houseflies were exposed to Cu2+. We found that adding Cu2+ to the larval diet inhibited larval growth, while the mixed addition of P. aeruginosa Y12 and Cu2+ to the diet reduced the inhibitory effects of Cu2+ on larval growth. Oral administration of Cu2+ significantly changed the gut community structure and increased larval gut bacterial diversity. In vitro analysis revealed that P. aeruginosa Y12 showed Cu2+ adsorption effects and increased Cu2+ aggregation. The mixed addition of low concentrations of P. aeruginosa Y12 and Cu2+ to the larval diet caused a dynamic shift in the gut microbiota and resulted in a novel gut community structure with an increase in beneficial bacteria and a decrease in pathogenic bacteria. Furthermore, P. aeruginosa Y12 treatment influenced the activity of antioxidant enzymes in housefly larvae, indicating that the addition of P. aeruginosa Y12 to the larval diet could further influence the antioxidant system through P. aeruginosa Y12-Cu2+ interactions. In conclusion, our research revealed that intestinal flora dysbiosis was the essential reason why copper inhibits housefly larval growth. However, proper supplementation with P. aeruginosa Y12 played positive roles in regulating larval gut communities and protecting insects from toxic heavy metals.http://www.sciencedirect.com/science/article/pii/S0147651323004827Heavy metal accumulationNatural systemToxicityHouseflyIntestinal floraPseudomonas aeruginosa |
| spellingShingle | Shumin Wang Kexin Zhang Qian Zhang Ying Li Yansong Yin Wenjuan Liu Sha An Ruiling Zhang Zhong Zhang Pseudomonas aeruginosa Y12 play positive roles regulating larval gut communities when housefly encountered copper stress Ecotoxicology and Environmental Safety Heavy metal accumulation Natural system Toxicity Housefly Intestinal flora Pseudomonas aeruginosa |
| title | Pseudomonas aeruginosa Y12 play positive roles regulating larval gut communities when housefly encountered copper stress |
| title_full | Pseudomonas aeruginosa Y12 play positive roles regulating larval gut communities when housefly encountered copper stress |
| title_fullStr | Pseudomonas aeruginosa Y12 play positive roles regulating larval gut communities when housefly encountered copper stress |
| title_full_unstemmed | Pseudomonas aeruginosa Y12 play positive roles regulating larval gut communities when housefly encountered copper stress |
| title_short | Pseudomonas aeruginosa Y12 play positive roles regulating larval gut communities when housefly encountered copper stress |
| title_sort | pseudomonas aeruginosa y12 play positive roles regulating larval gut communities when housefly encountered copper stress |
| topic | Heavy metal accumulation Natural system Toxicity Housefly Intestinal flora Pseudomonas aeruginosa |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323004827 |
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