Effects of Pesticide Application on the Growth of Soil Nitrifying Bacteria

This research studied the effects of pesticide application on the growth of soil nitrifying bacteria. Nitrosomonas sp. was isolated from the Soil samples gotten from five different locations within the University of Benin, Benin City and cultured using Winogradsky medium. The bacterial isolate was...

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Main Authors: U Udochukwu, AO Dave-Omoregie, HC Akaluzia
Format: Article
Language:English
Published: Joint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP) 2018-04-01
Series:Journal of Applied Sciences and Environmental Management
Subjects:
Online Access:https://www.ajol.info/index.php/jasem/article/view/169348
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author U Udochukwu
AO Dave-Omoregie
HC Akaluzia
author_facet U Udochukwu
AO Dave-Omoregie
HC Akaluzia
author_sort U Udochukwu
collection DOAJ
description This research studied the effects of pesticide application on the growth of soil nitrifying bacteria. Nitrosomonas sp. was isolated from the Soil samples gotten from five different locations within the University of Benin, Benin City and cultured using Winogradsky medium. The bacterial isolate was characterized and identified based on its cultural, morphological and biochemical characteristics. After 4 to 5 days of growth, Nitrosomonas Counts obtained from Winogradsky medium ranged from 4.8×103 to 9.2×103cfu/g. There was an increase in bacteria growth at lower pesticide concentration which began to decrease as the pesticide concentration increased with time. The percentage nitrite accumulation gradually decreased with time, the LC50 values were higher than EC50 values which shows that the bacteria could survive and grow at lower pesticide concentrations but were completely inhibited at higher pesticide concentrations. It is therefore very important to examine pesticides so as to determine its toxicity to soil bacteria before application.
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spelling doaj.art-754ef818a6c248318bb4fa191695e7602024-04-02T19:55:51ZengJoint Coordination Centre of the World Bank assisted National Agricultural Research Programme (NARP)Journal of Applied Sciences and Environmental Management2659-15022659-14992018-04-0122310.4314/jasem.v22i3.2Effects of Pesticide Application on the Growth of Soil Nitrifying BacteriaU UdochukwuAO Dave-OmoregieHC Akaluzia This research studied the effects of pesticide application on the growth of soil nitrifying bacteria. Nitrosomonas sp. was isolated from the Soil samples gotten from five different locations within the University of Benin, Benin City and cultured using Winogradsky medium. The bacterial isolate was characterized and identified based on its cultural, morphological and biochemical characteristics. After 4 to 5 days of growth, Nitrosomonas Counts obtained from Winogradsky medium ranged from 4.8×103 to 9.2×103cfu/g. There was an increase in bacteria growth at lower pesticide concentration which began to decrease as the pesticide concentration increased with time. The percentage nitrite accumulation gradually decreased with time, the LC50 values were higher than EC50 values which shows that the bacteria could survive and grow at lower pesticide concentrations but were completely inhibited at higher pesticide concentrations. It is therefore very important to examine pesticides so as to determine its toxicity to soil bacteria before application. https://www.ajol.info/index.php/jasem/article/view/169348Pesticidenitrosomonas sp.winogradsky mediumnitrite accumulation.
spellingShingle U Udochukwu
AO Dave-Omoregie
HC Akaluzia
Effects of Pesticide Application on the Growth of Soil Nitrifying Bacteria
Journal of Applied Sciences and Environmental Management
Pesticide
nitrosomonas sp.
winogradsky medium
nitrite accumulation.
title Effects of Pesticide Application on the Growth of Soil Nitrifying Bacteria
title_full Effects of Pesticide Application on the Growth of Soil Nitrifying Bacteria
title_fullStr Effects of Pesticide Application on the Growth of Soil Nitrifying Bacteria
title_full_unstemmed Effects of Pesticide Application on the Growth of Soil Nitrifying Bacteria
title_short Effects of Pesticide Application on the Growth of Soil Nitrifying Bacteria
title_sort effects of pesticide application on the growth of soil nitrifying bacteria
topic Pesticide
nitrosomonas sp.
winogradsky medium
nitrite accumulation.
url https://www.ajol.info/index.php/jasem/article/view/169348
work_keys_str_mv AT uudochukwu effectsofpesticideapplicationonthegrowthofsoilnitrifyingbacteria
AT aodaveomoregie effectsofpesticideapplicationonthegrowthofsoilnitrifyingbacteria
AT hcakaluzia effectsofpesticideapplicationonthegrowthofsoilnitrifyingbacteria