Improved spatiotemporal resolution of anti-scattering super-resolution label-free microscopy via synthetic wave 3D metalens imaging
Super-resolution (SR) microscopy has dramatically enhanced our understanding of biological processes. However, scattering media in thick specimens severely limits the spatial resolution, often rendering the images unclear or indistinguishable. Additionally, live-cell imaging faces challenges in achi...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Editorial Office of Opto-Electronic Journals, Institute of Optics and Electronics, CAS, China
2023-12-01
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| Series: | Opto-Electronic Science |
| Subjects: | |
| Online Access: | https://www.oejournal.org/article/doi/10.29026/oes.2023.230037 |
| _version_ | 1827347408685629440 |
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| author | Yuting Xiao Lianwei Chen Mingbo Pu Mingfeng Xu Qi Zhang Yinghui Guo Tianqu Chen Xiangang Luo |
| author_facet | Yuting Xiao Lianwei Chen Mingbo Pu Mingfeng Xu Qi Zhang Yinghui Guo Tianqu Chen Xiangang Luo |
| author_sort | Yuting Xiao |
| collection | DOAJ |
| description | Super-resolution (SR) microscopy has dramatically enhanced our understanding of biological processes. However, scattering media in thick specimens severely limits the spatial resolution, often rendering the images unclear or indistinguishable. Additionally, live-cell imaging faces challenges in achieving high temporal resolution for fast-moving subcellular structures. Here, we present the principles of a synthetic wave microscopy (SWM) to extract three-dimensional information from thick unlabeled specimens, where photobleaching and phototoxicity are avoided. SWM exploits multiple-wave interferometry to reveal the specimen’s phase information in the area of interest, which is not affected by the scattering media in the optical path. SWM achieves ~0.42 λ/NA resolution at an imaging speed of up to 106 pixels/s. SWM proves better temporal resolution and sensitivity than the most conventional microscopes currently available while maintaining exceptional SR and anti-scattering capabilities. Penetrating through the scattering media is challenging for conventional imaging techniques. Remarkably, SWM retains its efficacy even in conditions of low signal-to-noise ratios. It facilitates the visualization of dynamic subcellular structures in live cells, encompassing tubular endoplasmic reticulum (ER), lipid droplets, mitochondria, and lysosomes. |
| first_indexed | 2024-03-07T23:49:10Z |
| format | Article |
| id | doaj.art-75506ddfb213435a91c3b94afa05e744 |
| institution | Directory Open Access Journal |
| issn | 2097-0382 |
| language | English |
| last_indexed | 2024-03-07T23:49:10Z |
| publishDate | 2023-12-01 |
| publisher | Editorial Office of Opto-Electronic Journals, Institute of Optics and Electronics, CAS, China |
| record_format | Article |
| series | Opto-Electronic Science |
| spelling | doaj.art-75506ddfb213435a91c3b94afa05e7442024-02-19T09:02:48ZengEditorial Office of Opto-Electronic Journals, Institute of Optics and Electronics, CAS, ChinaOpto-Electronic Science2097-03822023-12-0121111010.29026/oes.2023.230037oes-2023-0037Improved spatiotemporal resolution of anti-scattering super-resolution label-free microscopy via synthetic wave 3D metalens imagingYuting Xiao0Lianwei Chen1Mingbo Pu2Mingfeng Xu3Qi Zhang4Yinghui Guo5Tianqu Chen6Xiangang Luo7National Key Laboratory of Optical Field Manipulation Science and Technology, Chinese Academy of Sciences, Chengdu 610209, ChinaNational Key Laboratory of Optical Field Manipulation Science and Technology, Chinese Academy of Sciences, Chengdu 610209, ChinaNational Key Laboratory of Optical Field Manipulation Science and Technology, Chinese Academy of Sciences, Chengdu 610209, ChinaNational Key Laboratory of Optical Field Manipulation Science and Technology, Chinese Academy of Sciences, Chengdu 610209, ChinaNational Key Laboratory of Optical Field Manipulation Science and Technology, Chinese Academy of Sciences, Chengdu 610209, ChinaNational Key Laboratory of Optical Field Manipulation Science and Technology, Chinese Academy of Sciences, Chengdu 610209, ChinaNational Key Laboratory of Optical Field Manipulation Science and Technology, Chinese Academy of Sciences, Chengdu 610209, ChinaNational Key Laboratory of Optical Field Manipulation Science and Technology, Chinese Academy of Sciences, Chengdu 610209, ChinaSuper-resolution (SR) microscopy has dramatically enhanced our understanding of biological processes. However, scattering media in thick specimens severely limits the spatial resolution, often rendering the images unclear or indistinguishable. Additionally, live-cell imaging faces challenges in achieving high temporal resolution for fast-moving subcellular structures. Here, we present the principles of a synthetic wave microscopy (SWM) to extract three-dimensional information from thick unlabeled specimens, where photobleaching and phototoxicity are avoided. SWM exploits multiple-wave interferometry to reveal the specimen’s phase information in the area of interest, which is not affected by the scattering media in the optical path. SWM achieves ~0.42 λ/NA resolution at an imaging speed of up to 106 pixels/s. SWM proves better temporal resolution and sensitivity than the most conventional microscopes currently available while maintaining exceptional SR and anti-scattering capabilities. Penetrating through the scattering media is challenging for conventional imaging techniques. Remarkably, SWM retains its efficacy even in conditions of low signal-to-noise ratios. It facilitates the visualization of dynamic subcellular structures in live cells, encompassing tubular endoplasmic reticulum (ER), lipid droplets, mitochondria, and lysosomes.https://www.oejournal.org/article/doi/10.29026/oes.2023.230037super-resolutionanti-scatteringunlabeledhigh temporal resolution |
| spellingShingle | Yuting Xiao Lianwei Chen Mingbo Pu Mingfeng Xu Qi Zhang Yinghui Guo Tianqu Chen Xiangang Luo Improved spatiotemporal resolution of anti-scattering super-resolution label-free microscopy via synthetic wave 3D metalens imaging Opto-Electronic Science super-resolution anti-scattering unlabeled high temporal resolution |
| title | Improved spatiotemporal resolution of anti-scattering super-resolution label-free microscopy via synthetic wave 3D metalens imaging |
| title_full | Improved spatiotemporal resolution of anti-scattering super-resolution label-free microscopy via synthetic wave 3D metalens imaging |
| title_fullStr | Improved spatiotemporal resolution of anti-scattering super-resolution label-free microscopy via synthetic wave 3D metalens imaging |
| title_full_unstemmed | Improved spatiotemporal resolution of anti-scattering super-resolution label-free microscopy via synthetic wave 3D metalens imaging |
| title_short | Improved spatiotemporal resolution of anti-scattering super-resolution label-free microscopy via synthetic wave 3D metalens imaging |
| title_sort | improved spatiotemporal resolution of anti scattering super resolution label free microscopy via synthetic wave 3d metalens imaging |
| topic | super-resolution anti-scattering unlabeled high temporal resolution |
| url | https://www.oejournal.org/article/doi/10.29026/oes.2023.230037 |
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